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Showing: 1 - 20 of 163 results
  1. pBA2675

    Plasmid
    #189997
    Purpose
    Inducible expression of FBP75 (1ā€“200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)
    Depositor
    Inserts
    FBP75
    VhhGFP4
    Use
    Expression vector for trypanosoma brucei
    Tags
    NLS
    Expression
    Mutation
    N-terminal 600 bp of FBP75
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pBA2705

    Plasmid
    #189998
    Purpose
    Inducible expression of FBP75(1ā€“200)-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for cytoplasmic proteins)
    Depositor
    Inserts
    FBP75
    VhhGFP4
    Use
    Expression vector for trypanosoma brucei
    Tags
    Expression
    Mutation
    N-terminal 600 bp of FBP75
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. PP-ASL

    Plasmid
    #40749
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    Trypanosoma Brucei Adenylosuccinate Lyase
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pUB39

    Plasmid
    #26300
    Depositor
    Insert
    VSG117 (Lister 427-4) cDNA
    Use
    Trypanosome inducible expression vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein iā€¦
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pBSVSG221KOblast

    Plasmid
    #59733
    Purpose
    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.
    Depositor
    Insert
    VSG221 knockout construct
    Use
    For homologous recombination into vsg221 expressiā€¦
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. TBSAHA

    Plasmid
    #42436
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    TBSAHA
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. 6NEY

    Plasmid
    #127295
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Insert
    PP-BRD3
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  9. pRPa-c6MYCn

    Plasmid
    #69242
    Purpose
    Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.
    Depositor
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Expression
    Mutation
    Promoter
    RRNA promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pRP-c6MYCn

    Plasmid
    #69243
    Purpose
    Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.
    Depositor
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Expression
    Mutation
    Promoter
    RRNA promoter
    Availability
    Academic Institutions and Nonprofits only
  11. PP-LeuAP

    Plasmid
    #40750
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    M17 aminopeptidase
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. PP-GNPNAT1

    Plasmid
    #25281
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    Tb11.01.2886:V5-L147
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. 6NIM

    Plasmid
    #127294
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Insert
    PP-BRD18
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 163 results