We narrowed to 201 results for: trypanosoma

1. Phleo/V5

   Plasmid

   #1809

   Depositor

   Christian Tschudi

   Article

   Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

   Insert

   Phleo/V5
   

   Tags

   V5

   Expression

   Bacterial

   Available Since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

2. neo/BB2

   Plasmid

   #1810

   Depositor

   Christian Tschudi

   Article

   Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

   Insert

   Neo/BB2
   

   Tags

   BB2

   Expression

   Bacterial

   Available Since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

3. BB2/neo

   Plasmid

   #1811

   Depositor

   Christian Tschudi

   Article

   Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

   Insert

   BB2/neo
   

   Tags

   BB2

   Expression

   Bacterial

   Available Since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

4. Cas9/pTREX-n

   Plasmid

   #68708

   Purpose

   Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
   

   Use

   CRISPR

   Tags

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

   Mutation

   C4239T mutation that eliminates a BamHI restricti…

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-Hyg

   Plasmid

   #48994

   Purpose

   Gateway adapted transfer of Hygromycin resistance gene

   Depositor

   Rick Tarleton

   Article

   Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

   Insert

   hygromycin B phosphotransferase
   

   Use

   Gateway attb1 & attb2

   Available Since

   April 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDONR221-Neo

   Plasmid

   #48993

   Purpose

   Gateway adapted transfer of Neomycin resistance gene

   Depositor

   Rick Tarleton

   Article

   Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

   Insert

   aminoglycoside 3' phosphotransferase
   

   Use

   Gateway attb1 & attb2

   Available Since

   April 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMOTag-glmS-4H

   Plasmid

   #106378

   Purpose

   Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

   Depositor

   Roberto Docampo

   Article

   Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

   Insert

   GlmS-3xHA
   

   Available Since

   March 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. p177bp-LacOx50-Hyg

   Plasmid

   #210213

   Purpose

   Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p177bp-LacOx162-Hyg

   Plasmid

   #210215

   Purpose

   Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   162xLacO repeats derived from: PMID: 12864855.
   

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBES1-LacOx50-Hyg

    Plasmid

    #210261

    Purpose

    Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLew100::NLS-ISceI-HA

    Plasmid

    #21299

    Depositor

    Nina Papavasiliou

    Article

    Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

    Insert

    I-SceI (SCEI )
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBES1-LacOx50-blast

    Plasmid

    #210221

    Purpose

    Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrex-Luc-Neo

    Plasmid

    #48336

    Purpose

    Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

    Depositor

    Rick Tarleton

    Article

    Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

    Insert

    firely luciferase
    

    Use

    Trypanasoma cruzi expression

    Promoter

    T. cruzi rRNA

    Available Since

    July 20, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMOTag-M9-4H

    Plasmid

    #106379

    Purpose

    Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation

    Depositor

    Roberto Docampo

    Article

    Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

    Insert

    GlmS with M9 mutation
    

    Available Since

    March 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLew100_myc_BirA*

    Plasmid

    #41716

    Depositor

    Graham Warren

    Article

    Morriswood et al Eukaryot Cell. 2012 Dec 21.

    Type

    Empty backbone

    Use

    T. brucei expression

    Promoter

    PARP

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pTcINDEX-GW

    Plasmid

    #234713

    Purpose

    Used to express any CDS in T. cruzi in a tetracycline regulatable manner. Modified from Taylor et al. (2006) for easy cloning using Gateway recombination cassette.

    Depositor

    Esteban Serra

    Article

    Alonso et al Mem Inst Oswaldo Cruz. 2014 Dec;109(8):1

    Type

    Empty backbone

    Use

    Trypanosomatid expression

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. TbSIR2RP1

    Plasmid

    #67242

    Purpose

    Determine its activity and specificity

    Depositor

    Cynthia Wolberger

    Article

    Fahie et al FEBS J. 2009 Dec;276(23):7159-76. doi: 1

    Insert

    TbSIR2RP1
    

    Tags

    MBP, TEV, Thrombin, HIS, and none

    Expression

    Bacterial

    Promoter

    p-lac

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pBES1-LacOx50-mCh-bla

    Plasmid

    #210260

    Purpose

    Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    mCherry
    

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC_sgRNA

    Plasmid

    #68710

    Purpose

    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Available Since

    Sept. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSY45

    Plasmid

    #48355

    Purpose

    Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    loxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies
    

    Use

    Cre/Lox; Knockout in t. brucei

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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