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Addgene
Showing: 41 - 60 of 174 results

  14. pHJ32

    Plasmid
    #48361
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48361-plasmid-map-sequence-id-70052

    Enlarge

  15. pHJ33

    Plasmid
    #48362
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48362-plasmid-map-sequence-id-70053

    Enlarge

  17. pDS67

    Plasmid
    #48365
    Purpose
    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48365-plasmid-map-sequence-id-71676

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  20. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    68710-plasmid-map-sequence-id-123992

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Showing: 41 - 60 of 174 results