389 results

1. Mouse lipid metabolism knockout library

   Pooled Library

   #206248

   Purpose

   The lipid metabolism library contains guide RNAs against 295 genes targeting mouse genes encoding enzymes that directly impact lipid metabolism.

   Depositor

   Kivanc Birsoy

   Article

   Soula et al Nature. 2024 Aug 7. doi: 10.1038/s41586-

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

2. Mouse Brie kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

   Pooled Library

   #75317

   Purpose

   Mouse sgRNA library in backbone lentiCRISPRv2 targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controls

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

3. hCRISPRa-v2, genome-wide, 10 sgRNAs/gene

   Pooled Library

   #1000000091

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of human genes

   Depositor

   Jonathan Weissman

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Mouse Metabolism Library

   Pooled Library

   #163966

   Purpose

   Lentiviral CRISPR library targets 2,918 mouse metabolic genes with ~18,000 guide RNAs. The library contains ~6 gRNAs per gene.

   Depositor

   Alec Kimmelman

   Article

   Biancur et al Cell Metab. 2020 Oct 28. pii: S1550-4131

   Use

   CRISPR and Lentiviral

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

5. Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility

   Pooled Library

   #101931

   Purpose

   Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility. Coexpresses mCherry.

   Depositor

   Michael Bassik

   Article

   Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

   Expression

   Mammalian

   Use

   CRISPR

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

6. Mouse SLC KO Library

   Pooled Library

   #141436

   Purpose

   Expresses gRNAs for knockout of the mouse Solute Carrier family

   Depositor

   Giulio Superti-Furga

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   CRISPR and Lentiviral

   Available Since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

7. Human Kinase Domain-Focused CRISPR Knockout Library

   Pooled Library

   #117725

   Purpose

   gRNAs targeting the kinase domain of human protein kinases

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

8. Yeast Inducible CRISPRi Library

   Pooled Library

   #161829

   Purpose

   ATc inducbile yeast CRISPRi library

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Expression

   Yeast

   Species

   Saccharomyces cerevisiae

   Use

   CRISPR

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pre-associated oBC-minP-GFP-mBC backbone (p043)

   Pooled Library

   #194098

   Purpose

   pre-associated oBC-minP-GFP-mBC backbone (p043), can directly be used to deposit library of regulatory elements

   Depositor

   Jay Shendure

   Article

   Lalanne et al bioRxiv 2022.12.10.519236

   Expression

   Bacterial and Mammalian

   Use

   RNAi

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

10. BARBEKO sgRNA library

    Pooled Library

    #174163

    Purpose

    iBARed cytosine Base Editing-mediated gene KnockOut (BARBEKO) sgRNA library; This library leverages CRISPR cytosine base editors for genome-scale knockout screens.

    Depositor

    Wensheng Wei

    Article

    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

11. Mouse genome-wide lentiviral CRISPR gRNA library version 2

    Pooled Library

    #67988

    Purpose

    Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Species

    Homo sapiens, Mus musculus, and Synthetic

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

12. scQers promoter control #1 (low: minimal promoter, p027)

    Pooled Library

    #217420

    Purpose

    scQers promoter control #1 (low: minimal promoter, p027)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pSADB19-dTomato Barcode Library

    Pooled Library

    #227671

    Purpose

    RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of SADB19 strain G-deleted rabies virus.

    Depositor

    Cathryn Cadwell, Tomasz Nowakowski

    Article

    Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

    Expression

    Mammalian

    Species

    Synthetic

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

14. TRE-MPRA_minPromega Library

    Pooled Library

    #201013

    Purpose

    Massively parallel reporter assay (MPRA) library with minPromega promoter

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Expression

    Mammalian

    Use

    Luciferase

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

15. Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

    Pooled Library

    #136479

    Purpose

    Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

    Depositor

    Jonathan Weissman

    Article

    Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

16. FNLCR CRISPRa Cell Surface

    Pooled Library

    #207471

    Purpose

    CRISPR-based activation library with guide RNAs targeting all cell surface proteins.

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

17. scQers promoter control #5 (high: EEF1A1 promoter, p028)

    Pooled Library

    #217424

    Purpose

    scQers promoter control #5 (high: EEF1A1 promoter, p028)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

18. Human sgRNA library Gattinara in pRDA_118 backbone

    Pooled Library

    #136986

    Purpose

    Expresses sgRNAs targeting human genes

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

19. Human DNA Binding Domain-Focused CRISPR Knockout Library

    Pooled Library

    #123334

    Purpose

    Expresses gRNA targeting the DNA binding domain of human transcription factors.

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

20. Bassik lab Mouse CRISPR-Cas9 Deletion Library - Gene Expression

    Pooled Library

    #1000000123

    Purpose

    CRISPR gRNA mouse knockout pooled library - Gene expression

    Depositor

    Michael Bassik

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only