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We narrowed to 438 results for: ERG

Showing: 1 - 50 of 438 results

  1. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...viral service items we employ dozens of pieces of energy-intensive lab equipment on a daily basis. By the...allows a community like Watertown to bulk purchase energy from a higher percentage of renewable sources. ... drawing 100% of our electricity from renewable energy projects right here in New England- the majority...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical... or substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will... will allow us to maintain these particularly energy-intensive systems in a more environmentally responsible...

  2. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scientists I’ve met began doing research in their undergraduate years. We each have a unique “origin story,”... provide some perspective and advice to any undergraduate interested in getting a taste of the role of...Professor Sometimes teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc...choosing one as their official thesis lab Undergraduate student Roles and responsibilities can vary...would also highly recommend talking to other undergraduate researchers, as well as graduate students (your...’s something you want to dedicate your time and energy to. How can I make the most of my experience in...valuable for anyone in the lab though, including undergrads. I found a lot of career resources on Science...

  3. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with... what is now commonly termed a Course-based Undergraduate Research Experience (CURE). Having incorporated...Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze their ...original research experiences for thousands of undergraduates. The MCC develops and makes freely available...production.   CUREs are beginning to transform undergraduate education in the sciences by making a greater... State College in New Hampshire. He mentors undergraduates conducting NIH- and NSF-funded research on ...Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences can make scientific research...

  4. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    ...Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an open-access journal that enables...Addgene. Listen to the interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter ... Springel, Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter 2 - Getting high school...

  5. Pipetting Protocol

    Type
    Protocol
    ... lower the pipette tip into the liquid. Do not submerge the pipette itself into the liquid and avoid touching...contaminating the contents. Once the end of the tip is submerged, slowly release the plunger, allowing it to go...

  6. Pouring LB Agar Plates

    Type
    Protocol
    ... a water bath at 60 ℃ with sufficient water to submerge ~75% of the bottle containing your molten gel ...removing anything from the autoclave. Partially submerge your molten gel-mix in the 60 ℃ water bath. Pro-Tip...

  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab. For more information, visit Addgene’s Mammalian... pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For more information, visit Addgene’s Mammalian...

  11. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the cells twice a week during...

  12. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...

  13. Brain Initiative Collection

    Type
    Collection
    ...-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...

  14. Brain Armamentarium

    Type
    Collection
    ...CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862...plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214857...) Expression of ChR2(H134R)-EYFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 213947...GCaMP6f AAV construct expressing GCaMP6f driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_... mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer Gordon Fishell James... mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer Gordon Fishell Viviana...

  15. CRISPR Guide

    Type
    Collection
    ...cleavage. Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding...stability PEmax - optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced editing... ssisted T argeting), developed by Samuel H. Sternberg’s lab . Many of these systems are based on naturally...argeting E lements), developed by the Abudayyeh - Gootenberg lab , also builds off prime editing. PASTE uses...Welch, M. M., Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J.... S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., Oura, S., Holmes, B., Tanaka...Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O.,...

  16. Plasmids for Stem Cell Research

    Type
    Collection
    ...Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons...fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons...Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion...conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS...10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable...to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts...neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons Lentiviral Human Direct Conversion...

  17. Fluorescent Proteins: FRET

    Type
    Collection
    ... Fluorescence) Resonance Energy Transfer (FRET) is a process by which energy is non-radiatively transferred...resonance energy transfer mCerulean, mVenus, and Amber (mVenus Y67C) constructs for measuring energy transfer...microscopy (FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor lifetime...et al. (2006) Quantitative understanding of the energy transfer between fluorescent proteins connected...acceptors Koushik et al. (2009) Anomalous surplus energy transfer observed with multiple FRET acceptors ... protein acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Targeted sensors for glutamatergic neurotransmission Targeted sensors for glutamatergic neurotransmission...fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET) efficiency of a pair of fluorescent...Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer...Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics...Fluorescent sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor...and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of... acetylcholine sensor for monitoring in vivo cholinergic activity (GACh3.0) (Constitutive or Cre-dependent...

  19. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson 83441 pSOD1E133insTT-AcGFP1...TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV ALS Shawn Ferguson 133755 pLVX-Puro-...TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP CMV ALS Shawn Ferguson 133757 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP CMV ALS Shawn Ferguson 133759 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP CMV ALS Shawn Ferguson 133761 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43...U6 ALS Shawn Ferguson 221553 pPB-EF1A-Puro-hTBK1-EGFP TBK1 GFP EF1-alpha ALS Shawn Ferguson 221554 pPB-...

  20. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...watermelon mutant clones for your course-based undergraduate research experience (CURE) projects....Community (MCC) Collection Blog post: Course-Based Undergraduate Research Experiences (CUREs) (Link opens in ...window) develops protein-centric Course-based Undergraduate Research Experiences (CUREs) centered on a single...opens in a new window) Length of course-based undergraduate research experiences (CURE) impacts student ...Increasing access for biochemistry research in undergraduate education: The malate dehydrogenase CURE community...

  21. Retrovirus Plasmids

    Type
    Collection
    ... immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV...Contains MoMLV gag and pol; use with envelope plasmid Weinberg 12371 pCL-Eco Packaging Contains MoMLV gag, pol...Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope...plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA...

  22. mTOR Pathway

    Type
    Collection
    ...synthesis, and increases energy production through glycolysis to fuel these energy-intensive processes. mTORC1...signals, including amino acids, stress, oxygen, energy levels, and growth factors. mTORC1 integrates these...including an abundant supply of macromolecules and energy, as well as the presence of both pro-proliferative...machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov...

  23. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...minBG-SYFP2-WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA...minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2...minBG-SYFP2-WPRE3-BGHpA AiP13038 AiE0743m_3xC2 SYFP2 Cholinergic interneurons Striatum 214848 pAAV-AiE0743m_3xC2...minBG-iCre(R297T)-BGHpA AiP13780 AiE0743m_3xC2 Cre Cholinergic interneurons Striatum 214858 pAAV-AiE0873m_3xC2...10aa-H2B-WPRE3-BGHpA AiP14496 AiE0873m_3xC2 SYFP2 Cholinergic interneurons Striatum 214868 pAAV-AiE0873m_3xC2...minBG-iCre(R297T)-BGHpA AiP15138 AiE0873m_3xC2 Cre Cholinergic interneurons Striatum 191706 pAAV-AiE0779m_3xC2...

  24. Lentivirus Plasmids

    Type
    Collection
    ...making Integrase deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and...N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag... with 2nd and 3rd generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for...resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven...

  25. Neurodegeneration Research Collection

    Type
    Collection
    ...-CRISPR/Cas9 strategy for gene editing across divergent rodent species. Boender et al. Sci Adv. 2023 Jun... Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Sun et al. Nat Methods. 2020...30. Enhance differentiation to mesencephalic dopaminergic (mesDA) neurons using gene knockout . Maimaitili...resource was created with support from the Chan Zuckerberg Initiative (Link opens in a new window) ....

  26. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. 2017. Enhanced...PMID: 27984730 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...Science . 343(6166):80-4. PMID: 24336569 Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost...

  27. Zhang Lab CRISPR Page

    Type
    Collection
    ...activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein... editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ...J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature . ...aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

  28. Antibody Production

    Type
    Collection
    ...production, all antibody preps are titered and undergo stringent quality control by Addgene or a trusted...Buffer After affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The...distributed. Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs...

  29. Promega Plasmid Collection

    Type
    Collection
    ... BRET energy donor and HaloTag protein, labeled with the NanoBRET 618 fluorophore, as the energy acceptor...Assays NanoBRET (Link opens in a new window) is an energy transfer technique designed to measure molecular...

  30. Deisseroth INTRSECT Collection

    Type
    Collection
    ...delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786. PubMed (...Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range..., Fuzik J, Fürth D, Fenno LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A...

  31. Luciferase Plasmid Collection

    Type
    Collection
    .... Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase... NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as...

  32. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 206555 Anti-Alpha-2C adrenergic receptor [N330A/80R] Alpha-2C adrenergic receptor Human Mouse IgG2a... Mouse IgG2a 220412 Beta-2 adrenergic receptor [N430/30R] Beta-2 adrenergic receptor Mouse Mouse IgG2a...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...scFv] N324/2 Rem2 Rat Mouse 219492 Anti-Alpha-2C adrenergic receptor scFv [N330A/80 scFv] N330A/80 Alpha-...

  33. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA...NgoMIV, AgeI, SpeI, PstI For more info, visit iGEM: Freiberg Standard (RFC 25) Back to Top...

  34. Immunology Research Plasmids and Resources

    Type
    Collection
    ...dJ579N16.4 ADRB1 adrenergic, beta-1-, receptor ADRB1R, B1AR, BETA1AR, RHR ADRB2 adrenergic, beta-2-, receptor...CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPR30, LERGU, LERGU2, LyGPR, MGC99678 GPHA2 glycoprotein hormone ...activates the T cell, whereas T cell tolerance or anergy are induced when the TCR is engaged without co-...LHRHR, LSH-R LIF leukemia inhibitory factor (cholinergic differentiation factor) CDF, DIA, HILDA LIFR ...

  36. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...antibodies. Detergents  Another common family of permeabilization reagents are detergents, such as Triton...these detergents avoid some of the negative effects caused by organic solvents. However, detergents can ...for what reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic...Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the... X-100 and Tween-20 are examples of non-ionic detergents that interact with lipids and proteins in membranes...protein. B) Permeabilizing with the selective detergent, saponin, keeps membrane-associated proteins in... epitopes. C) Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated...

  37. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA...with the discovery of CRISPR-transposons by the Sternberg and Zhang labs (Peters et al., 2017; Klompe et...Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated Transposase...the CRISPR-transposon systems developed by the Sternberg lab derives from Vibrio cholerae (V. cholerae ...CRISPR-transposons for bacterial genomes engineering In the Sternberg group’s initial report of VchINT, three separate...Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical Center. He ....     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA...

  38. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction between...fluorophore (the donor) is excited, it can transfer energy directly via resonance to the second fluorophore...fluorophore labeled and that these fluorophores can undergo FRET when they are near each other. How do you ...still develop a successful FRET-based assay since energy transfer only occurs when donor and acceptor are...acceptors >100 Å away from the donor doesn’t result in energy transfer and thus, your specific FRET signal is...donors never “see” FRET acceptors, and thus never undergo FRET. If this nonspecific labeling far exceeds ...experiments will define the maximum and minimum energy transfer values you can expect, thereby establishing...

  39. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...molecule is excited by a high-energy photon and re-emits a lower-energy photon; no chemical reaction takes...) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy from an excited donor fluorophore...Bioluminescence Resonance Energy Transfer (BRET) works the same way, except that energy is transferred from ...Fluorescence Source of light Chemical reaction High-energy photons Kinetics of photon generation Slower ...interaction brings the fluorescent protein close enough, energy is transferred by BRET and the fluorescent protein...

  40. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...have acquired divergent functions due to mutations accumulated over time? Undergraduates can help to answer...providing authentic research experiences to undergraduate students (1, 2), but educators face significant...Time project provides a framework for these undergraduate research questions. The idea is straightforward...cerevisiae. The two yeasts are thought to have diverged from a common ancestor ~350 million years ago.... have been largely conserved since the species diverged. Tips on using the Pathways Over Time plasmids... in strategies to incorporate research into undergraduate courses and in communicating science to the ...Advancement of Science. (2011). Vision and Change in Undergraduate Biology Education: A Call to Action, Washington...

  41. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described by Theodor... the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation and emission...fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted photons) from the excited donor...fluorophore excitation by absorption of a photon 2. Energy transfer from the excited donor directly to the...spectrum of the acceptor fluorophore. Otherwise the energy transferred from the donor will not be able to ...of each other and be oriented appropriately for energy transfer via dipole-dipole interaction. The efficiency...M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission in living...

  42. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...the photon's energy is lost, so the energy of an emitted photon is lower than the energy of the absorbed...wavelengths are higher in energy, while photons of higher wavelengths are lower in energy. As such, wavelengths...they promote electrons within atoms to a higher energy state. This process is called excitation. After...a short period, the electrons revert to a lower energy state, whereby a photon is emitted. This step is... due to a process called Fluorescence Resonance Energy Transfer (FRET).   Figure 1: Emission spectra... relationship between a photon's wavelength and energy, how photons can be absorbed and emitted by matter...

  43. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., 2017 and 2018). While the Doudna lab ...not been approved for diagnostics yet, but is undergoing validation studies.     Jennifer Tsang contributed...updating this article. References Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT...439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy...442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins...10.1126/science.aaq0179 Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner...Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann...

  44. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...other reagents in biology. Luk H. Vandenberghe, PhD Luk Vandenberghe’s first lab experience was in a virology... Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re excited to grow the team and bring on...worked on vaccines throughout that time. Now Vandenberghe is an Associate Professor at Mass Eye and Ear... to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions with...ultimately make innovation and science slow,” Vandenberghe says. “The continuous creativity that Addgene...Karen Guerin for the journal Molecular Therapy. Vandenberge is also the Grousbeck Family Chair in Gene Therapy...

  45. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...Plasmids: History & Biology The Joshua Lederberg Papers Joshua Lederberg's Personal Perspective: Plasmid (1952...Pubmed PMID: 13003535. Lederberg, Joshua, Luigi L. Cavalli, and Esther M. Lederberg. “Sex Compatibility ...did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these...the Fertility, or F-factor discovered by Ester Lederberg in 1952 was noted to integrate into the E. coli...PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis...

  46. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    Interesting...well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique which allows the detection ..."a distance-dependent physical process by which energy is transferred non-radiatively from an excited ... may be higher than the signal generated by the energy transfer. On the contrary, if you choose a pair... development of new fluorescent pairs and the emergence of faster measurement systems, this technique .... Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell...

  47. Bacterial Expression Systems

    Type
    Collection
    ...Return to top Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they are within a...

  48. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...CRISPR-transposon system before another exciting new advance emerges! The programmability and targeting power of CRISPR...the directed evolution expertise of the Samuel Sternberg and David Liu labs. Evolving CASTs for new contexts...plasmids here! The new horizons of evoCAST In the emerging world of gene therapy, highly tailored treatments... H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial genome engineering using..., S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded CRISPR-Cas systems...L. H., Chen, A. T., Tolar, J., Osborn, M. J., Sternberg, S. H., & Liu, D. R. (2025). Programmable gene...

  49. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...summer internship information sessions to help undergraduate students with their applications. The society...society currently has 1,283 members, who comprise undergraduates, graduate students, postdocs, faculty members...The society encourages interactions between undergraduates and PhD students so that they can help support...society currently has 352 members, ranging from undergrad all the way up to professors. Oxford Biological...at Imperial College London for students in this emerging field. It has gained over 170 members in its first...or biological societies were you a part of in undergrad or grad school? How did these societies help (...

  50. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...3rd 10 Varies Berger Lab Human Paralog Knockout Library (pgPEN) 171172 Knockout Human Berger 3rd 8-16 33,170...
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