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We narrowed to 881 results for: NSI

Showing: 1 - 50 of 881 results

  1. Genetic Code Expansion

    Type
    Collection
    ...Code Expansion Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible...regulate the activity of a protein so that it is responsive to specific stimuli, such as light. On a broader... the genetic code, there are several things to consider. If you are working off of a previously established...phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein...

  2. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...cell-to-cell communications and environmental sensing. Sensing and Signaling Plasmids Search the table by...Biology plasmids for Sensing and Signaling. Plasmid... Plasmid Collections Synthetic Biology Sensing and Signaling Synthetic...Synthetic Biology: Sensing and Signaling SynBio Resources SynBio Home Browse All SynBio Assembly Standards...Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection...

  3. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...Kebabian, John W., and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible role in synaptic transmission...Gary L. Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain...

  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...equipment needed for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...done about that.  Second, expansion introduces distortions. The goal in expansion is for your sample to expand...maximizing label density. In some cases, nanobodies have been found to help improve label density (Mikhaylova... Bournonville L, et al (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria...Expansion Microscopy (ExM) promises an easier, more accessible way to image biological features previously... ExM images in a new publication.         Why Expansion Microscopy?  Traditional light microscopes are...samples can be expanded to ~4-10x linearly! This expansion physically separates molecules while maintaining...

  5. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...was aware that such roles existed, but did not consider them for myself. I started picturing myself in...technical support specialist for 4 years. Read on for insights from my time in technical support including tips...takes some time (several years) to build up comprehensive lab experience. With this valuable training ...any incoming requests or problems - there is considerable diversity in the types of questions customers...individual stories and get (sometimes refreshing) insights into how other scientists think and work. Most...drive to help others. These roles offer an easy transition from an academic lab to industry without being...

  8. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...s post, we’ll discuss some of the important considerations you should think about when developing optogenetics...place aversion also models reinforcement but is considerably simpler than ICSS. The test chamber is divided... of the type of behavior, certain technical considerations still apply. Testing stimulation First, In ...cue. The hippocampus has long been known to be responsible for mediating this behavioral effect but the ...mouse; however, all the steps and technical considerations are directly applicable to the rat [6]. Technical...PMC3150193. 8.Han, Xue, et al. "A high-light sensitivity optical neural silencer: development and application...

  10. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical amino...introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and techniques...number of experiments with. The catch The rapid expansion of GCE led to the creation of many new tools and...meaning that the final protocol may involve a sensitive balance of trade-offs. This balance can be extremely...Tan, Z., & Cornish, V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry...

  11. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...development of transient transformation systems for other species as well. Things to consider in developing...generating a transgenic plant can take even longer, a transient plant leaf transformation process could save the...transgenic plant takes even longer, is more labor intensive, inefficient, and often not reproducible. Prior...to creating transgenic cultures or plants, our transient expression method enables us to quickly screen...biosynthetic pathway. Why plant scientists do transient leaf transformations The development of transgenic...common problem in non-model organisms. Therefore, transient methods are usually the first choice for investigating...instead of months to years. In the plant sciences “transient transformation” usual means that neither a stable...

  12. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...Understanding the transition While a PhD equips you with exceptional technical skills, transitioning into management...   Strategies for a successful transition To successfully transition into a managerial role, it's essential...mentors who have made similar transitions can provide you with insights, lessons from their experiences... most pivotal decisions many PhDs face when transitioning from academia to industry is whether to stay...preparation and a willingness to embrace new responsibilities. After my second postdoc, I realized that...career trajectories within the industry, but the transition wasn’t easy - I had to reinvent myself and redefine...d like to share below. Common pitfalls in the transition (what I wish I knew before experimenting on myself...

  13. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...should be an important consideration during any experiment. Sensitivity Cas9 sensitivity is often closely related... important factors to consider: site accessibility, specificity, and sensitivity. Site accessibility Site...Another important consideration when choosing a Cas9 is balancing specificity and sensitivity. Wild-type Cas9..., accessibility, specificity, and sensitivity are key considerations in choosing a Cas protein for your...appropriate for your specific research question. Considerations when choosing your Cas nuclease When choosing... related to specificity. Sensitivity is a measure of the on-target activity of the Cas9 nuclease. This... measure of off-target activity (left) while sensitivity is the measure of on-target activity (right)....

  14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... as channelrhodopsins (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing...tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain is a tricky organ...mm3). Current channelrhodopsins also need high-intensity light for activation which requires implantation...design new channelrhodopsins that are more light sensitive and have enhanced conductance compared to current...channelrhodopsin’s photocurrent strength, wavelength sensitivity, and off-kinetics.   This model was then used... measured photocurrent amplitude, wavelength sensitivity, and off-kinetics correlated with the model’s... produced stronger photocurrents at low light intensity than existing channelrhodopsins CoChR and/or ChR2...

  15. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...technique! What is a photosensitizer? First, for a few definitions. A photosensitizer is a dye or protein...system? Turns out that with light-inducible photosensitizers and a quick zap of the proper color light,...light, you can do just that.  Photosensitizing dyes and proteins have been around for awhile (check out this...Bruchez and Tsang labs recently developed a photosensitizer composed of the protein complex complex and... oxygen species (ROS). The ROS produced by photosensitizers inactivate target proteins or cells while ...having little effect on neighboring cells. The photosensitizer used in this work is composed of two parts:...fluorogen (the TAP - targeted and activated photosensitizer - MG-2I in this case). The fluorogen is not...

  16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...to individual researchers. Yet accurate and comprehensible organization of data is essential for efficient...store raw data in formats that require specific (expensive) software that evolves over time such that prior... the journals themselves should be primarily responsible for storage.  If datasets are too large to transfer...projection of it, could allow idea generation and extension, based on existing, published data. The importance...However, expectations must also be reasonable, sensitive to the data type and made as facile for both the...

  17. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...researchers, we wondered how we could make the insights gained during this time more visible and thereby... ReFigure saves time, knowledge and makes your insights discoverable. Watch this quick video to learn ... ground again and again as they don’t save the insights they glean from a reading session. To remember...notes on a “Pinterest for scientists”? ReFigure extension is a simple Chrome plugin that allows you to save...papers to find the right experiment! The ReFigure extension also tells readers if a paper they’re reading ...ReFigure making it easy for others to discover your insights on a topic. Beyond collating other’s data, you...instantly and are editable by the author at any time. Insights contained in your ReFigure could be beneficial...

  18. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...and quantify based on band intensities of your DNA relative to the intensity in the ladder. It’s best to...things to consider: cost, time, equipment, and expected DNA concentration. It’s important to consider the advantages...any special reagents. However, there’s limited sensitivity at low concentrations of DNA and it can’t distinguish...measure all nucleic acids). These methods are more sensitive than UV absorbance, especially when you expect... to compare the band intensity of the fragment in the ladder that is closest in size to your piece of ...nm. This method is time consuming and has low sensitivity so it’s not used much. However, the measurements...

  24. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring mechanical tension across vinculin...7303):263-6. Martin Schwartz Tension Talin tension sensors Talin tension sensor reveals novel features...F40-based tension sensor modules Grashoff Lab Tension Sensor Plasmids Carsten Grashoff Tension tCRMod tension... tension sensors Tunable molecular tension sensors reveal extension-based control of vinculin loading.... cAMPFIRE cAMP biosensors with improved sensitivity Sensitive genetically encoded sensors for population...highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like...transmission and mechanosensitivity. J Cell Biol. 2016 May 9;213(3):371-83. Martin Schwartz Tension HP35-, FL...

  25. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...amino acid linker ACA Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Amber...linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Venus VCA Heterotrimeric...Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Amber VCV Heterotrimeric construct...construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct consisting... linker-Venus ACVA Heterotetrameric construct consisting of Amber-5aa linker-Cerulean-5aa linker-Venus... linker-Amber VCAA Heterotetrameric construct consisting of Venus-5aa linker-Cerulean-5aa linker-Amber... linker-Amber VCVV Heterotetrameric construct consisting of Venus-5aa linker-Cerulean-5aa linker-Venus...

  26. Tetracycline Inducible Expression

    Type
    Collection
    ...type of transduction (transient or stable) may depend on the desired sensitivity, level of induction, ...Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids Inducible and repressible promoters...has been modified to provide two classes of tet-responsive systems: Tet-Off and Tet-On. Read on to learn...components of these systems for better efficiency, sensitivity, and control of expression. These include: Tet-On...rtTA-Advanced, also known as rtTA2 S -M2) with greater sensitivity to doxycycline and three copies of a minimal ...variants with lower background activity and greater sensitivity to doxycycline (Zhou et al., 2006). Tet-On 3G...interactions with endogenous factors. Experimental Considerations Expression System If your gene of interest ...

  27. Antibody Guide

    Type
    Collection
    ...Figure 1. The two arms of the Y structure are responsible for binding the antigen, and are called the antigen...isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can change the constant...binding site. In B cells, the genetic region responsible for the variable region goes through a randomized...valency of 2 IgE Protects against parasites and is responsible for driving allergic reactions such as anaphylactic...requirements, monoclonal antibodies are typically more expensive than polyclonal antibodies. Environmental factors... Minibodies are engineered antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments... protein. Fluorophores Fluorophores are light-sensitive molecules which emit a specific wavelength of ...

  28. Plan Your Experiment

    Type
    Collection
    ...Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt...essentially function as a “knockout,” but be sure to consider potential consequences of having a non-functional...be no PAM site close to your desired edit site. Consider alternative Cas9 enzymes with different PAM sequences...completely avoid them. To increase specificity, consider using an engineered Cas enzyme with higher fidelity...to off-target activity, it is also important to consider factors that maximize cleavage of the desired ...with BioRender.com. When choosing your system, consider which Cas enzyme you are using (as species and...for your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...

  29. Immunology Research Plasmids and Resources

    Type
    Collection
    ... proteins consists of Type I, Type II, and Type III interferons. They trigger the defensive responses ..., WBCQ1 DEFA1 defensin, alpha 1 DEF1, DEFA2, HNP-1, HP-1, MGC138393, MRS DEFA3 defensin, alpha 3, neutrophil-specific..., HP-3 DEFA5 defensin, alpha 5, Paneth cell-specific DEF5, HD-5, MGC129728 DEFB1 defensin, beta 1 BD1,...MGC51822 DEFB103A defensin, beta 103A DEFB103, DEFB3, HBD-3, HBD3, HBP-3, HBP3 DEFB104A defensin, beta 104A ...

  30. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...erythroleukemia (MEL) cells, a suspension cell line. The culture medium in all steps consists of DMEM supplemented...eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient genome editing tool that...off-target effects). Ensure that the guide sequences consist of a 20-mer (“protospacer sequence”) upstream of..., resolve agarose gel to determine if size is consistent with the presence or absence of deletion. For...L-glutamine, which is used for MEL cells. However, transient transfection of CRISPR/Cas9 plasmids may be successfully...strategies for each cell type. While MEL cells are suspension cells, instructions for adherent cells have ...validation (see step 6 ). NOTE: This protocol is for suspension cells. Adherent cells can either grow as individual...

  31. COVID-19 Resources

    Type
    Collection
    ...receptor for SARS-CoV-2 and SARS-CoV is hACE2, an angiotensin receptor. Another key step is the priming of ...to identify antibody escape mutants and for comprehensive mapping. Some of the following plasmids are ...the links and references below: ACE2 - (Human Angiotensin Converting Enzyme 2) is the host cell receptor...SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably detect nucleic acids at...and optimize the method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2...available from Xiaokun Shu's lab. A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for... Library of Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor...

  32. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...MCP tagged plasmids for visualization of proteins inside cells....ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated ...visit the New England Biolabs website. For a comprehensive comparison to GFP, please refer to NEB's comparison...Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control...Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control...pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector...Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid...

  33. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...plasmid backbone to use, you have many elements to consider. Here is a guide to a selection of Addgene's empty...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian...gene with N-terminal GST tag and TEV cleavage Transient expression - Gradia Lab plasmids for bacterial...EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging...other common uses. Viral Vector Delivery Although transient expression is sufficient for some experiments,...integrate into the host genome Addgene has an extensive collection of plasmids for packaging and expression...Lentiviral backbones made by Addgene Addgene has an extensive collection of plasmids for packaging and expression...

  34. Bacterial Expression Systems

    Type
    Collection
    ...Genome Engineering You may also like… Genetic Code Expansion Collection Microbiology Plasmids Collection Synthetic... Consortium (SGC) collection page contains an extensive selection of plasmids and tags for protein purification...Visit our Fluorescent Protein Guide to find an extensive collection of FPs, including photoactivatable,...for non-toxic protein labeling and visualization inside living or fixed bacterial and mammalian cells. ...Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL (Quorum sensing molecule) Escherichia coli Cynthia Collins 84689...Loris 47663 pAC-EsaR-I70V Plac 3OC6HSL (Quorum sensing molecule) Escherichia coli Cynthia Collins 112197... (Tet) Inducible Expression Collection for an extensive selection of plasmids using tetracycline-regulated...

  35. AAV Packaged on Request

    Type
    Collection
    ...Viral vector production Density centrifugation purification Addgene’s comprehensive quality control process... amplify DNA, produce the prep, and perform comprehensive quality control processes to make sure it is...vector experience, from methods to troubleshooting insights, you make research more reproducible for scientists... that complement published data, capturing the insights that often go unshared. Help Center Check out ...

  36. CRISPR Guide

    Type
    Collection
    ...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...There are two main types of base edits: Base transitions - edit to same classification of nucleotide base...pyrimidine-to-pyrimidine), which catalyze base transitions. Cytosine base editors are created by fusing ...integrated along with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases... target gene function. It has also been used extensively to screen for novel genes that regulate known...simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single transcriptional...silencing across much of the human genome following transient expression of the dCas9 fusion, with the epigenetic...

  37. Viral Vector Guides and Plasmids

    Type
    Collection
    ...capacity, high transduction efficiency, and high but transient transgene expression.They can induce a strong ...RNA < 8 kb 80–130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼4.5 kb ...Dividing/Non-dividing No* Very Low Adenoviral Transient Double-stranded linear DNA 5–36 kb 105 nm Dividing...divided into multi-plasmid systems. These systems consist of a plasmid containing the genetic information...

  38. Promega Plasmid Collection

    Type
    Collection
    ...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...collection contains luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors... measure subtle expression changes with high sensitivity. These assays are ideal for real-time monitoring...bright and stable luciferase that enables highly sensitive detection at endogenous expression levels. NanoLuc...

  39. Luciferase Plasmid Collection

    Type
    Collection
    .... pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (superecliptic pHluorin), a pH-stable... Plasmid Collection Luciferase is the enzyme responsible for bioluminescence found in a variety of organisms... the emission of photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching...

  40. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Plasmids MORF Pooled Library The MORF library is a comprehensive human transcription factor collection that contains...Pooled Library The MORF pooled library is a comprehensive collection of human transcription factor open...

  41. Synthetic Biology - Overview

    Type
    Collection
    ...as logic gates and higher level gene networks. Sensing and Signaling Browse plasmids related to intercellular...intercellular signaling and environmental sensing. Strains Browse Addgene's collection of engineered bacterial...Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB...

  42. Antibody Production

    Type
    Collection
    ...and stained with Coomassie blue. Protein band intensities are measured using ImageJ software and a standard...standard curve. Using ImageJ software, the band intensities of both the antibody and impurities are measured...anti-GFP antibody), the target antigen is first transiently expressed in mammalian cells. To learn which ...

  43. Antibody Plasmid Collection

    Type
    Collection
    ...yeast, plants, or insect cells. Benefits include consistency between lots and the ability to optimize the ... NeuroMab collection — monoclonal antibodies extensively validated for neuroscience research applications...Recombinant mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells...

  44. mTOR Pathway

    Type
    Collection
    ...production through glycolysis to fuel these energy-intensive processes. mTORC1 also blocks catabolic processes...substrate 1 (proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related...known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent companion of MTOR...

  45. Plant Plasmids and Resources

    Type
    Collection
    ...transcription factors for Agrobacterium-mediated transient expression or plant transformation - Silin Zhong...CRISPR-mediated Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in...interdisciplinary exchange, open technologies, and responsible innovation for improvement of sustainable agriculture...

  46. Viral Production

    Type
    Collection
    ...staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed. The...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...

  47. Lentivirus Plasmids

    Type
    Collection
    ...transgene expression. There are some safety considerations to evaluate when working with lentivirus: these... genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems...1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible...

  48. Neurodegeneration Research Collection

    Type
    Collection
    ...speaking and swallowing. HD is caused by abnormal expansion of a repeating CAG triplet series within the huntingtin...or way to prevent progression of PD. Treatment consists mainly of managing symptoms through medication...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell...

  49. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... ). The phosphorylation sites (“phosphosites”) consist of a central phosphoserine site flanked by 15 amino...Additional Resources See our dedicated Genetic Code Expansion collection page Read about Using Phosphoserine...Phosphorylation Read about GCE4All: Making Genetic Code Expansion Accessible...

  50. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...empty backbones or reporters . Experimental Considerations Recombinase Recognition Sites Each enzyme functions...in-stock viral preps feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options...in-stock viral preps feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options...
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