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We narrowed to 247 results for: GRN

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Showing: 201 - 220 of 247 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . . PMID: 27088723...

  2. CRISPR Plasmids for Genomic Visualization

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    ...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers...it is easy to implement due to the simplicity of gRNA design, programmable for different genomic loci,...efficiently visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  3. CRISPR Plasmids - dCas9-FokI

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    ...Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

  4. CRISPR Plasmids - Activate Gene Expression

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    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  5. CRISPR Plasmids - Parasites

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    Collection
    ...protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...Library , a Toxoplasma gondii genome-wide knockout gRNA pooled library designed with 10 guides against 8,158...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  6. CRISPR Plasmids - Purify Genomic Loci

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    ...genomic DNA bound by the gRNA. An epitope tag or tags can be fused to dCas9 or gRNA for efficient purification...of any genomic sequence specified by a particular gRNA. In the enChIP (engineered DNA-binding molecule-...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  7. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...use in Xenopus. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  8. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...use in zebrafish. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  9. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... in C. elegans. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  10. CRISPR Plasmids - Drosophila

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    Collection
    ... other insects. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  11. CRISPR Plasmids - Plants

    Type
    Collection
    ... use in plants. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  12. CRISPR Plasmids - Yeast

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    ...for use in yeast. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...use in bacteria. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  14. CRISPR Plasmids - Single-Strand Break (Nick)

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    Collection
    ...Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of ...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  15. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...expression systems. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  16. CRISPR Plasmids - Double-Strand Break (Cut)

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    Collection
    ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  17. CRISPR Plasmids - Cascade-Cas3

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    Collection
    ...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. After nicking the non-targeted...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  18. CRISPR Plasmids and Resources

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    Collection
    ...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...

  19. Lentivirus Plasmids

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    ...to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...

  20. CRISPR Plasmids - Repress Gene Expression

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    Collection
    ...transcription. This interference is most efficient when the gRNA targets your gene of interest’s promoter, enhancer...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...
Showing: 201 - 220 of 247 results