We narrowed to 794 results for: Tec

...For rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics: DETECTR (Link opens...tools, like Cas12a and Cas13a plasmids, to reliably detect nucleic acids SARS-CoV-2 main protease (Mpro) activity...protease, and polymerase constructs for SARS-CoV-2 detection assays Neutralizing nanobody plasmids And more...Addgene blog Finding nucleic acids with SHERLOCK and DETECTR No Llamas Required - Synthetic Nanobodies Against...

... heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be ...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 ...

... of detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like...imaging has numerous advantages over other imaging techniques, including that it is easy to implement due to...FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living cells. The fluorescent...

...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

...99222 pTECH-chPylRS(IPYE) PylRS Chimeric Nε-boc-lysine (BocK) Bacterial TAG Dieter Söll 104069 pTECH-chAcK3RS...104070 pTECH-MbAcK3RS(IPYE) AcK3RS M. barkeri Nε-acetyl-L-lysine Bacterial TAG David Liu 104071 pTECH-MmAcK3RS...104072 pTECH-MbPylRS(IPYE) PylRS M. barkeri Nε-boc-lysine (BocK) Bacterial TAG David Liu 104073 pTECH-MmPylRS...

...popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus plasmid...split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent protein-peptide interactions...kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library ...phosphorylation-dependent interactions . Nature Biotechnology , 36 (7), 638–644. https://doi.org/10.1038/nbt...

...binding of Tet-Advanced transactivator (developed by Clontech, based on Resnitzky et al., 1994). TRE3G promoter...alternative effectors for Tet transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04...promoters with minimized background expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10...opens in a new window) Tet Systems: Science and Technology (Link opens in a new window) Content last reviewed...

...can be safely handled at the lowest biosafety protection levels. High quality and high titer in-stock ...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed...

...editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID:...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different... analysis of CRISPR–Cas experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587...

...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample — just ...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...

... Proteins Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein...intellectual property rights, and provides liability protection for material providers. Addgene's deposit agreements...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information...

...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

...spanning a wide variety of molecular biology technologies: from genome engineering and gene expression...luminescent, and color-producing proteins for detection of gene expression, protein localization, protein–protein...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ...

...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

... luciferases require different substrates and detection wavelengths. Firefly luciferase is brighter than...investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating...investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5'...

...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of...

...you choose and use antibodies, with an expert technical support team ready to answer your questions. All... antibody production and storage techniques, and explain common applications. View Guide ...

...Additional Resources Addgene's Data Hub Curated technical reports from the research community demonstrating...troubleshooting advice and explanations of viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors...

...inclusive, ‘open-access ethos’ to the results, techniques, and reagents with the highest-possible quality... research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project...

..."), creating over 100,000 unique combinations detectable by antibodies. All of the PRISM protein tags ...individual or subsets of barcode proteins to label and detect multiple injection sites in the same tissue, as...

...exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA — additional protection added ...

...CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR...resources Bacterial CRISPR Resources The CRISPR technology was developed from bacterial immune systems, ...

...CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources include plasmids... website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's...

...can be safely handled at the lowest biosafety protection levels. We offer 1,077 in-stock viral vector ...well-developed and trusted protocols and peer-reviewed techniques (Link opens in a new window) for producing high-quality...

...neurons function. They are also delving into new technologies and resources in order to gain more fundamental...mitochondrial DNA using mitoBEs . (Yi et al. Nat Biotechnol. 2023) Initiate heritable gene silencing with...

...traditional tagging methods. Several different tagging techniques, as well as the plasmids and protocols needed...Förstemann lab has developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate...

...dividing cells. CRISPR activation and interference technologies also indirectly modify local chromatin state...

...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

... CRISPR Plasmids and Resources CRISPR technologies are continously developing and expanding. Addgene...

...constructs and routine protein and molecular biology techniques. Using a single protein with many available isoforms...

...papers on Syn Bio SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource Synthetic...

...such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods...

... within this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing...

... ddPCR AAV Titration by qPCR Using SYBR Green Technology Content last reviewed: 10 September 2025 Do you...

...immunology, biochemistry, and more. The foundational technologies enabled by this collection include polymerase...

...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

...microscopy or spectroscopy by exciting the donor and detecting the emission of the acceptor. Alternatively, the...