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We narrowed to 715 results for: cas

Showing: 21 - 40 of 715 results

  1. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems... Tools CRISPR Blog Posts Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  2. CRISPR Plasmids - Base Edit

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    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...the fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both ... lab and CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase...

  3. Plant Plasmids and Resources

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    ...Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA...MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice. ... an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre...CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector...development. The TapRoot - Podcast (Link opens in a new window) - The Taproot is a podcast to understand how scientific...plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples of useful...

  4. CRISPR Plasmids - Epigenetics

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    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target...

  5. CRISPR Plasmids - Repress Gene Expression

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    ...target the dCas9-repressor to your specific locus. Want more information on the wide variety of Cas enzymes...Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

  6. CRISPR Plasmids - RNA Editing

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    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with...VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts...guanosine, the result is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence...

  7. CRISPR Plasmids - Empty gRNA Vectors

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    ...that of the Cas protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems... CRISPR requires that you have to express both a Cas protein and a target-specific gRNA in the same cell.... Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their function... plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can cut, activate... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering...

  8. CRISPR Plasmids - Activate Gene Expression

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    ...target the dCas9-activator to your specific locus. Want more information on the wide variety of Cas enzymes...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

  9. CRISPR Plasmids - RNA Targeting

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    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with...sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have...to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation...

  10. CRISPR Plasmids for Genomic Visualization

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    ...CRISPR/Cas plasmids for labeling and visualization of genomic loci.... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  11. CRISPR Plasmids - Parasites

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    ...help of Scott Lindner . Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  12. CRISPR Plasmids - gRNAs

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    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence...

  13. Zhang Lab CRISPR Page

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    ...Lab General Cloning Protocol 237.2 KB SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian...Return to top References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran...hosts contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase...Protocols 2013). SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing ...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA...

  14. Fluorescent Protein Guide: Biosensors

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    ...Sensor for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1....Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...calcium sensor T-GECI Blue-shifted genetically encoded Ca(2+) indicator with enhanced two-photon absorption...green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun...fluorescent CEPIA indicators for visualization of Ca(2+) dynamics in mitochondria. Sci Rep. 2020 Feb 18...CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian neurons. Science. 2017 ...Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins...

  15. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...possibilities for Cas proteins With their enhanced specificity, eSpCas9, SpCas9-HF1, and HypaCas9 should enable...specificity of the Cas9 nuclease with engineered variants: eSpCas9, SpCas9-HF1, & HypaCas9. The off-target...portions of Cas9 might be important for nuclease specificity. They found that eSpCas9(1.1) and SpCas9-HF1 each...sites tested when compared to eSpCas9(1.1) and SpCas9-HF1. The HypaCas9 plasmids are available from Addgene...off-target effects” have included using a Cas9 nickase variant, lowering Cas9 expression, and truncating gRNA sequences... target effects of CRISPR/Cas9 by altering the nuclease activity of the Cas9 nuclease itself. Design and... and testing of eSpCas9 Looking at the structure of the Cas9 nuclease (PDB ID: 4OO8 and 4UN3), Slaymaker...

  16. Worm Expression Resources

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    ...found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids... via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous...that incorporate a self-excising drug selection cassette (SEC). More information regarding this kit can...targeting vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR ...that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens in a new window...

  17. Degrading DNA with Cascade-Cas3

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    ... A multitasking Cas Most Cas effectors have one job — Cas see DNA, Cas chop DNA. Cas3, however, has two...CRISPR 101: Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems INTEGRATE...CRISPR has brought many other Cas effectors to light. One lesser-known Cas is waiting for its chance in...and Cas11. Type I-E Cascades contains all five of these Cas proteins, while type I-C Cascades do not contain... the spotlight — Cas3. Cas3 can induce genomic alterations on a scale that other Cas effectors can’t, ...a multi-Cas complex called Cascade (CRISPR-associated complex for antiviral defense). Cascade is brought...complex” system Cas3 is part of the Class 1 CRISPR family, which means it requires multiple Cas effectors to...

  18. CasPEDIA: A Functional Classification of Cas Enzymes

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    ... for a candidate Cas for your experiment. Target requirements As we all know, a Cas guided by a gRNA ...properties for a Cas entry (SpyCas9a) from CasPEDIA.   How can I use it? CasPEDIA operates like many...Addgene and CasPEDIA have got you covered!     Fig. 1 – CasPEDIA Cas ID display information for...for a given Cas (SpyCas9a) from CasPEDIA.   Helpful resources and information For some researchers,...CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) The PAM Requirement...Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...What are the features? CasPEDIA has collected relevant information on all the Cas enzymes you know and ...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas family of proteins...targeting. RNA targeting Cas enzymes Move over Cas9, CRISPR isn’t just for DNA anymore! Cas enzymes have been... facts on Cas family proteins and genome editing abilities   Choosing a Cas While Cas9 may be the...the most adaptable Cas currently available, the other Cas proteins should not be overlooked. The unique...DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes is its...delivery.   Cas14 – Mini, but single strand mighty Unlike many other Cas enzymes, Cas14 targets ssDNA...lesser-known Cas enzymes while also highlighting their similarities and differences to Cas9. DNA targeting...

  20. Zebrafish Plasmid Collection

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    ...Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful...Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes...use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated...
Showing: 21 - 40 of 715 results