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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 650 results for: CHI;

Showing: 61 - 90 of 650 results

  2. Mammalian RNAi Tools

    Type
    Collection
    ...a tool in mammalian cell systems, silencing is achieved through the delivery of a double-stranded RNA ...

  3. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine...

  4. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles...

  5. CRISPR Plasmids - Yeast

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...

  6. mTOR Pathway

    Type
    Collection
    ...PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg...

  7. Microbiology Resources

    Type
    Collection
    ...External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (Link opens...

  8. Plant Plasmids and Resources

    Type
    Collection
    ...MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan et al., 2007) and includes four promoters...diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression...With eSpCas9(1.1) and coselection, Agudelo et al. achieved editing rates as high as 83% with NHEJ and 40-...shortening leads to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation...

  10. Open Enzyme Collection

    Type
    Collection
    ...codon-optimized the Open Enzymes parts for expression in Escherichia coli bacteria. Researchers can use the collection...

  11. Ras Pathway

    Type
    Collection
    ...targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed ...

  12. Lentivirus Plasmids

    Type
    Collection
    ...pLionII 3rd FIV-derived (FELIX) plasmid with a chimeric 5’LTR, Any expression cassette (promoter and gene...

  14. Rett Syndrome

    Type
    Collection
    ...Methyl-CpG binding protein 2. Am J Med Genet B Neuropsychiatr Genet . 180, 55–67. PMID: 30536762 Shahbazian...

  15. Plan Your Experiment

    Type
    Collection
    ...reagents may be sufficient to express the CRISPR machinery. For more difficult cells (e.g. primary cells)...

  16. DNA Quantification

    Type
    Protocol
    ...Blanking' measures the background inherent to the machine and your solvent. If using a NanoDrop to measure...

  21. Water Bath Protocol

    Type
    Protocol
    ...can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room temperature....

  22. Centrifugation

    Type
    Protocol
    ...separate different components in a liquid sample. It achieves this by using centrifugal force, which is generated...

  23. Pipetting Protocol

    Type
    Protocol
    ...submerge the pipette itself into the liquid and avoid touching the pipette to the inside of the tube. Only the...

  25. Kit Free RNA Extraction

    Type
    Protocol
    ... (Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window) TRIzol...

  27. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on power is...thanks to our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...to select a photostable fluorescent protein. Photobleaching is the irreversible destruction of a fluorophore...fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins may display reversible...Shaner et al, 2008; Bindels et al, 2017) and photoswitching (Kremers et al, 2009), which usually are undesired...destroyed. Factors that affect photostability The photobleaching rate of a fluorophore primarily depends on ...

  28. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...cells.   Many thanks to our guest bloggers Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe ...Leiwe and Takeshi Imai from Kyushu University. Richi Sakaguchi is a graduate student at Kyoto University. ...25157152. PubMed Central PMCID: PMC4246963. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright...light microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the...Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced, fluorescent...you use AAVs, it typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the ...

  29. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...resulting in the following hierarchical model: Because the U.S., Europe, and China account for over 75% of...instabilities in the China data, so we recommend interpreting the model results for China with more uncertainty... dominated by the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests correspond to ...encountered COVID-19 at different times. Most notably, China was affected by COVID-19 earlier than the rest of...COVID-19 event windows as 01/15/20 -- 03/15/20 for China and 03/11/20 -- 05/19/20 (the last date in our dataset...day across regions, with Europe most impacted and China least impacted. The inferred weekend effect is, ...well. However, we observed one complication in the China data – there is a period of 1 month, from 1/22/20...

  30. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...limited biological materials. Problems with Chip-seq ChIP-seq typically requires a large amount of input...enough material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  cross-linking...immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where a protein...in many model organisms and cell lines. Although ChIP-seq is a relatively simple and robust protocol it...patterns that overcomes some of the limitations of ChIP-seq. CUT&RUN, which stands for ‘Cleavage Under Targets... using the Drosophila wing as a model. A typical ChIP-Seq experiment requires around 1,000 wings. Collecting...All in all, CUT&RUN offers a great alternative to ChIP-seq, especially in situations when it is technically...
Showing: 61 - 90 of 650 results