We narrowed to 658 results for: CHI;

...bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove et al., Nature 1997 ) and...

...a tool in mammalian cell systems, silencing is achieved through the delivery of a double-stranded RNA ...

...have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine...

...PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg...

...External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (Link opens...

...process reliant on Flp recombinase. 5 Kazunari Miyamichi Genome Engineering Tools These AAV encode Cas9...

...codon-optimized the Open Enzymes parts for expression in Escherichia coli . Researchers can use the collection as ...

...based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan et al., 2007) and includes four promoters...diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression...With eSpCas9(1.1) and coselection, Agudelo et al. achieved editing rates as high as 83% with NHEJ and 40-...shortening leads to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation...

...targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed ...

...Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative...

...pLionII 3rd FIV-derived (FELIX) plasmid with a chimeric 5’LTR. Any expression cassette (promoter and gene...

...Methyl-CpG binding protein 2. Am J Med Genet B Neuropsychiatr Genet . 180, 55–67. PMID: 30536762 Shahbazian...

...SYN1 HA-hM3D(Gq) Syn Activator DREADD Christopher Richie 50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry Syn Activator...

...Blanking' measures the background inherent to the machine and your solvent. If using a NanoDrop to measure...

... allow enough time for the loop to cool before touching it to the bacteria. Gently spread the bacteria...

...possible. You will want nice crisp bands. This can be achieved by using a wider gel comb and running the gel ...

...necessary to get to 50μL. Efficient annealing can be achieved by one of two methods: Method #1 Place the mixed...

...can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room temperature....

...separate different components in a liquid sample. It achieves this by using centrifugal force, which is generated...

...submerge the pipette itself into the liquid and avoid touching the pipette to the inside of the tube. Only the...

...antibiotic, which may not be stable at 37 °C. To achieve a stable cell pool, the antibiotic selection should...

... (Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window) TRIzol...

...ILT5, LIR-3, LIR3, MGC138403, PIRB LYN v-yes-1 Yamaguchi sarcoma viral related oncogene homolog FLJ26625...

... 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on power is...thanks to our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...to select a photostable fluorescent protein. Photobleaching is the irreversible destruction of a fluorophore...fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins may display reversible...Shaner et al, 2008; Bindels et al, 2017) and photoswitching (Kremers et al, 2009), which usually are undesired...destroyed. Factors that affect photostability The photobleaching rate of a fluorophore primarily depends on ...

...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...cells.   Many thanks to our guest bloggers Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe ...Leiwe and Takeshi Imai from Kyushu University. Richi Sakaguchi is a graduate student at Kyoto University. ...25157152. PubMed Central PMCID: PMC4246963. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright...light microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the...Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced, fluorescent...you use AAVs, it typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the ...

...resulting in the following hierarchical model: Because the U.S., Europe, and China account for over 75% of...instabilities in the China data, so we recommend interpreting the model results for China with more uncertainty... dominated by the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests correspond to ...encountered COVID-19 at different times. Most notably, China was affected by COVID-19 earlier than the rest of...COVID-19 event windows as 01/15/20 -- 03/15/20 for China and 03/11/20 -- 05/19/20 (the last date in our dataset...day across regions, with Europe most impacted and China least impacted. The inferred weekend effect is, ...well. However, we observed one complication in the China data – there is a period of 1 month, from 1/22/20...

...limited biological materials. Problems with Chip-seq ChIP-seq typically requires a large amount of input...enough material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  cross-linking...immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where a protein...in many model organisms and cell lines. Although ChIP-seq is a relatively simple and robust protocol it...patterns that overcomes some of the limitations of ChIP-seq. CUT&RUN, which stands for ‘Cleavage Under Targets... using the Drosophila wing as a model. A typical ChIP-Seq experiment requires around 1,000 wings. Collecting...All in all, CUT&RUN offers a great alternative to ChIP-seq, especially in situations when it is technically...

...secondary antibody. Chicken IgY This RANbody reporter consists of two chicken IgY domains. Chicken IgY domains...antibodies. Chicken IgY RANbodies are easily detected with a labeled anti-chicken antibody. Chicken IgY RANbody..., H2A2B Myc Tagged RFP, H2A2B Chicken IgY (Y) Labeled Anti-Chicken IgY antibody Staining cultured cells...three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain, which are discussed in more detail ...the Key Features of HRP, Spaghetti Monster, and Chicken IgY Domain RANbody Reporters. Reporter Detection... anti-rodent or -rabbit Detection with an anti-Chicken IgY antibody is approximately as sensitive as detecting...

...FP-protein fusions (FP-chimeras) is that they undergo normal protein turnover. FP-chimeras are continuously ...degraded within the cell, so at any given time, an FP-chimeric protein may be at any one of many stages of synthesis...turnover or temporal expression using standard FP-chimeric proteins. Photoactivatable fluorescent proteins...bright state to a dark non-fluorescent state (“quenching”). The photoswitch can occur multiple times by...reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists used mutagenesis screens...V157G / M159Y / F190S) (7). Padron’s “positive” switching behavior is the opposite of Dronpa; when exposed...monomeric PA-FPs, there is less chance that the chimeric protein will oligomerize, disrupting cellular ...

...and most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler,... LUX fluorogenic primers Low Single self-quenching fluorescent molecule connected to a primer  ...previously-generated validation data, be aware that different machines, samples, and treatments may affect the assay....running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr chemistry is ... by bubbles in wells, variations in volume, or machine surge errors and allow for well-to-well normalization... likely need to re-optimize protocols for that machine. (Teo, et al, 2002; see paper for more information...