Skip to main content
Addgene
Showing: 61 - 90 of 813 results

  1. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...for expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  2. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...for expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  3. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...available for expression in mammalian systems, bacteria, and yeast. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  4. Fluorescent Protein Guide

    Type
    Collection
    ...conformational changes within a protein. Biosensors Monitor small biomolecules or other physiological intracellular... or sensors. Chemogenetics Plasmids | Guide Use small molecules to activate genetically engineered cellular...protein-protein interactions in organs and whole mammals. Regulate Biological Activity Use fluorescent proteins...

  5. AAV Viral Preps

    Type
    Collection
    ...delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...in-stock AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...

  6. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...DSB. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, C. elegans...elegans, yeast, zebrafish, and Xenopus. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

  7. Chemogenetics Plasmids

    Type
    Collection
    ...engineered receptors that are activated by specific small molecules, allowing a scientist to control signaling...engineered to respond to specific physiologically inert small molecules. There are four types DREADDs in Addgene...receptor (nAChR) domains that respond specifically to small molecules termed Pharmacologically Selective Effector...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...available for expression in mammalian systems, bacteria, and plants. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel Bruchez...Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisome Peroximal Targeting Signal 1 (PTS1) mCherry* Michael Davidson...Davidson 36203 pmTurquoise2-Peroxi Peroxisome Peroxisomal Targeting Sequence mTurquoise2 Dorus Gadella ...responsible for forming connections and adhesions between animal cells. They are important for holding cell structures...

  10. CRISPR Plasmids and Resources

    Type
    Collection
    ...for use in Drosophila . Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR...plasmids for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish...

  11. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...than 100bp away, ideally less than 10bp away. For small (<50bp) changes, you should consider using ssDNA...less than 10bp away if possible. For introducing small mutations (<50bp) or a single-point mutation, the...length in drosophila. This 1998 study suggests mammalian cells' tract lengths seem much shorter. What is...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...VWM disease Harmit Malik 20045 pSB819-PKR-hum-FFS EIF2AK2 GAL VWM disease Harmit Malik 20046 pSB819-PKR-...VWM disease Harmit Malik 20047 pSB819-PKR-hum-LYS EIF2AK2 GAL VWM disease Harmit Malik 20048 pSB819-PKR-...VWM disease Harmit Malik 20049 pSB819-PKR-hum-FYA EIF2AK2 GAL VWM disease Harmit Malik 20050 pSB819-PKR-...VWM disease Harmit Malik 20051 pSB819-PKR-hum-LFA EIF2AK2 GAL VWM disease Harmit Malik 20123 PCRII-zSynC...other Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial... Mark Mayford 35000 Nurr1 NR4A2 PGK Parkinson's Malin Parmar 35217 psen2_L (OZ577) PSEN2 lac UV5 Alzheimer's...

  13. Trimmer Lab NeuroMab Collection

    Type
    Collection
    .../28R] HCN1 Rat Mouse IgG2a 114513 Anti-Malin [N85/18.1R] Malin Human Mouse IgG2a 114514 Anti-DNMT3L [N117...by transiently transfecting the plasmids into mammalian tissue culture cells (e.g., HEK293, COS, CHO),...can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species...GSG2 Human Mouse IgG2a 206531 Anti-MBP [N128A/4R] Maltose binding protein (MBP) E. coli Mouse IgG2a 206532...can be used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target... as intrabodies and nanoscale immunolabels in mammalian brain neurons. eLife . 8:e48750. PMID: 31566565...

  14. TALEN Expression Vectors

    Type
    Collection
    ...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH...vectors listed below have the CMV promoter for mammalian cell expression and a T7 promoter for in vitro...

  15. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... with target sites throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

  16. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...disease in the world. Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African...

  17. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...protein-protein interactions in deep-tissue organs and whole mammals. Genetically encoded fluorescent probes are suitable...spatially selective imaging of tissues in living animals. Further development of the original iRFP has resulted...

  18. Plasmid Collections

    Type
    Collection
    ...Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi Microbiology Plant Expression Stem Cells ... with the same backbone and only differing in a small region. Click here to view all pooled libraries....

  19. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in this article...Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic Biology, 2(...

  20. Resolute Plasmid Collection

    Type
    Collection
    ...academic research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE... fluorescence, mass/charge properties, protein thermal stabilization, and changes of voltage all contribute...

  21. Synthetic Biology - Overview

    Type
    Collection
    ...Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper...Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing...

  22. Adenovirus Plasmids

    Type
    Collection
    ...recombination of adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and...This DNA molecule can then be transfected into mammalian packaging cell lines to generate adenovirus particles...

  23. Retrovirus Plasmids

    Type
    Collection
    ...inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN is a modified...-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you have suggestions...

  24. Antibody Production

    Type
    Collection
    ...Production For recombinant antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments ...

  25. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base...

  26. mTOR Pathway

    Type
    Collection
    ...mTOR Gene List Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is a key metabolic... enriched in brain S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous...

  27. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...AAV Plasmids Adeno-associated viruses (AAV) are small viruses originally discovered as contaminants of... The utility of AAV is currently limited by its small packaging capacity (∼4.5 kb including ITRs), though...

  28. Qi Lab CRISPR Page

    Type
    Collection
    ...CRISPR system for genome mutagenesis ( Cong et al. , Mali et al. , Jiang et al. , Hwang et al. ), is that ...targeted genes. 2. Two-plasmid CRISPRi system for mammalian gene knockdown The first plasmid ( pdCas9_humanized...

  29. AAV Packaged on Request

    Type
    Collection
    ...delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...Quantity Total Volume Minimum Titer Typical Titer Small 10 × 100 µL aliquots 1 mL 4 × 10 12 GC/mL* 1 × 10...

  30. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base...
Showing: 61 - 90 of 813 results