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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 910 results for: ire;

Showing: 61 - 90 of 910 results

  1. Rett Syndrome

    Type
    Collection
    ..., is regression of previously acquired skills, notably loss of acquired purposeful hand movements and ...Envigo. The following cell lines are available directly from the labs in which they were created. Find...rsrt.org The following cell lines are available directly from the labs in which they were created. Find... a p.R168X or p.T158M MECP2 mutation, and the direction and degree of skewing of X-chromosome inactivation...

  2. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...removing the amplification and extraction steps required when one receives plasmids in bacterial stabs....yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning...DNA is designed for use in cloning applications. Direct transfection into cells is not recommended. Can...independently including the check out and will require separate shipping charges. Where can I find cloning...

  3. Serotype Testing AAV

    Type
    Collection
    ...for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid 37825...37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information about...

  4. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions...plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids....

  5. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1) pAAV-EF1a-Flpo (55637-AAV1) Viral...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...promoter directs ubiquitous expression. pAAV-mDlx-NLS-mRuby2 (99130-AAVrg): The Dlx element directs expression...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: EGFP-expressing...pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV pCAG-FLEX-EGFP-...112677-AAV5 and 112677-AAVrg): The EF1a promoter directs strong, ubiquitous expression. This is a Cre-dependent...

  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...following in mind: CRISPR screening experiments require electroporation to amplify the pooled library and...library representation. CRISPR screening experiments require large quantities of cells and therefore, may not...limited (e.g. primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 ...of gRNAs - A given CRISPR library may target the entire genome or a specific class of genes (subpools)....Library 112200 Knockout Human Lin 3rd 4-5 8,382 Human Paired-guide RNA (pgRNA) Library for Long Non-coding RNAs...

  7. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...(PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the Cas9 nickase...that binds to a specific genomic DNA locus and directs the nickase to the target site. The longer pegRNA...3’ flap binds to the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription...

  8. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...interest and effort directed toward expanding this capacity. Traditionally, AAV requires the presence of ... products that mediate AAV replication. This requirement has been circumvented with “helper-virus free...(which may not be available at Addgene) may be required to produce infectious AAV particles. You can search...

  9. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...cell may instead repair the break using homology-directed repair (HDR). HDR is much less error-prone than...plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids....

  10. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids....

  11. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... and TniQ). Some type I CASTs can result in bidirectional insertions — meaning they may not be suitable...However, type I-F CASTs favor insertion in the T-RL direction, with the right homology end closest to the target... I, type V-K CASTs appear to exhibit almost unidirectional insertions, but are more prone to off-target...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...material. The mitochondria produces the energy cells required for chemical reactions. Labeling these structures...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999... alpha-tubulin mCherry* Michael Davidson 12298 pIRESneo-EGFP-alpha Tubulin Microtubules alpha-tubulin ... the proteins that enter the secretory pathway require chaperones to help with folding or post-translational...

  13. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...charges may be required before your order will be delivered. The courier will contact you directly regarding...additional customs documentation that might be required by your country for the import of biological research...

  14. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...vectors if you need to. Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection... of dollars, but by approaching it from a new direction, we hope to do it much faster. Years of science...

  15. Lentiviral Prep Service

    Type
    Collection
    ...library expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode...library expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode...library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse ...

  16. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...with two homology arms on each side flanking your desired insertion or mutation. Each arms is around 800bp... in the HR template. Since the PAM sequence is required for successful targeting and cleavage, changing...with two homology arms on each side flanking your desired insertion or mutation. Each arm is around 800bp...

  18. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...template to introduce specific edits via homology-directed repair (HDR). Want more information on the wide...

  19. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity...to limit the spread of infection throughout the entire population. Type VI enzymes that function in mammalian...

  20. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

  21. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...purification methods are more straightforward and enable direct identification of molecules associated with a genomic...CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...

  22. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...

  23. CRISPR Plasmids - Parasites

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...

  24. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion of the backbone...Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or Neurog2 ...

  25. TALEN Engineering

    Type
    Collection
    ... engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation...assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional pages ...

  26. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... not exist in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA...wider editing window, and are thus well suited to directed evolution applications. Examples of these base...

  30. TALEN Plasmids and Kits

    Type
    Collection
    ...higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter...domains (DD, RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins...
Showing: 61 - 90 of 910 results