...Anhydrotetracycline inducible expression of wild-type Cas9 from S. pyogenes for inducing double stranded ...coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence ... the MS2 binding sites within this plasmid. pZE31-DsRed-ms2x2 26248 MS2 coat protein binding sites Philippe...localization: Contains a 2x array of the binding sequence for RNA-binding protein MS2 fused to dsRED. Can be... Tac (lactose/IPTG inducible) MBP, 6x His David Waugh Empty lactose/IPTG inducible vector for expression.../IPTG inducible) C-terminal TEV cleavable 6x His Cheryl Arrowsmith Empty lactose/IPTG inducible vector...expression upon induction) lactose/IPTG Cheryl Arrowsmith Empty lactose/IPTG inducible vector. pPro Series...

... in a new window) . For more details, see Clement et al. (2019) (Link opens in a new window) . MAGeCK ...(Link opens in a new window) . gRNA Design DeepSpCas9 (Link opens in a new window) A webtool that uses...(Link opens in a new window) . Also try CRISPOR batch (Link opens in a new window) for lentiviral screens...the National Cancer Institute. Cas-OFFinder (Link opens in a new window) Identifies gRNA target sequences...Mateo (Link opens in a new window) Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA... in a new window) Labs. Off-Spotter: tool for CRISPR/Cas design (Link opens in a new window) Program for...opens in a new window) for more information. CRISPR MultiTargeter (Link opens in a new window) Can be used...

... tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre tet inducible Cre-...Tamoxifen inducible CAG Mammalian Cepko 15028 Hsp68-CreER(TM) (DM#264) CRE-ER(TM) - Tamoxifen inducible Hsp68...Tamoxifen inducible; Targeting vector for Pax7 Tanaka 111187 pBADZ-HisCre Cre; arabinose inducible. PBAD ...Tamoxifen inducible CAG Mammalian Capecchi 125749 pCAG-sfGFP-iCre-ERT2 iCre-ERT2 - Tamoxifen inducible CAG ...and recombination. Use Addgene's curated tables to find Cre-lox plasmids for your experiments. Plasmid...constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct...expression and to optimize Cre expression once it is induced. Addgene has a wide variety of Cre recombinase ...

...Acinar Cells induced Beta Cells Adenoviral Mouse Long-term persistence and development of induced pancreatic... Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells (iPSCs) in both mouse and...expression of key factors. The tables below will help you find plasmids available from Addgene that can be used...search the iPSC Neurodegenerative Disease Initiative (iNDI) Collection for plasmids that can be used to create...Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the table ...species, or PI and then select the article link to find more information. Delivery Method Species Description...and Lin28 from four separate lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human ...

...a new window) Brain Banks (Link opens in a new window) Rodent Models (Link opens in a new window) Michael...reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant...Foundation (Link opens in a new window) A foundation dedicated to finding a cure for Parkinson's disease... opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ... Addgene has assembled the following to help you find information and plasmid collections that may help...triplet series within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin...

.... gRNA binding induces a conformational change in Cas9, shifting it into an active, DNA-binding configuration...( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome ...composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines... otif (PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends...with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases...undergoes a second conformational change upon target binding. In this formation, its nuclease domains, RuvC ...causes small nucleotide insertions or deletions (indels) at the DSB site. Therefore, a population of cells...

...available to industry, in addition to academics and nonprofits, and this is denoted in the Industry column ...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing... SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI For more information...protocols and design resources (Link opens in a new window) Find Zhang Lab Cas13a plasmids Additional protocol...diagnostics: SARS-CoV-2 DETECTR (Link opens in a new window) Find Doudna Lab Cas12a plasmids Addgene's full CRISPR...Link opens in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that...like that of SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors...

... Tet-inducible Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression...Mammalian, Varies Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids...for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression vector URA3...fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable marker. They are... plasmids for mammalian expression, Ligation Independent Cloning (LIC) pcDNA3.1-mCherry - Expresses mCherry... plasmids for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression...

... increase its stability and DNA binding activity, and allow it to bind transcriptional co-activators and...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific...and DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like growth factor binding protein 3 KAI...protein p53 regulated apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B ...apoptosis effector PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and...these mutations are missense mutations in the DNA-binding domain, often at one of six hotspot residues. These... cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene...

...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Standard Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT...experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of novel ...plasmids. Online spreadsheet (Link opens in a new window) of Flp-recombinase-expressing transgenic mouse... maintains a public folder (Link opens in a new window) of manuscripts that have used these constructs...inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene...Methods 11(7):763-72. PubMed (Link opens in a new window) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, ...

...ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2, RAET1H ULBP3 UL16 binding protein...kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein...RING11 TAPBP TAP binding protein (tapasin) NGS17, TAPA, TPN, TPSN, tapasin TAPBPL TAP binding protein-like...factor beta binding protein 1 MGC163161 LTBP2 latent transforming growth factor beta binding protein 2 ... RAB GTPase binding effector protein 1 RAB5EP, RABPT5 RABEP2 rabaptin, RAB GTPase binding effector protein...cell is primed to recognize a specific antigen, it finds and attacks pathogens or infected cells expressing...T cell, whereas T cell tolerance or anergy are induced when the TCR is engaged without co-stimulation ...

...the ligand-binding pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific ...antibodies that uniquely: Bind to the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit... opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...Protein Innovation (IPI) Collection! Here, you’ll find unique recombinant antibodies targeting families...model. Learn more about IPI (Link opens in a new window) Glypican Antibodies Glypicans are a family of ...specific subunit pairs, blocking ligand binding and receptor function Figure courtesy of IPI Browse Integrin ...

...CAG Alzheimer's Dennis Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP ...Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed/Ind APP CMV Alzheimer's Aleksandra Radenovic 114410 AICSDP...Huntington's Susan Lindquist 1178 p416 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1179 p416 72Q GPD...Huntington's Susan Lindquist 1180 p416 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1181 p426 25Q GPD...Huntington's Susan Lindquist 1182 p426 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1183 p426 72Q GPD...Huntington's Susan Lindquist 1184 p426 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1185 p416 25Q GAL...Huntington's Susan Lindquist 1186 p416 103Q GAL HTT GFP GAL1 Huntington's Susan Lindquist 1187 p426 25Q GAL...

...enzyme, binds the shRNA and cleaves it to produce siRNA. The siRNA then binds the RNA-induced silencing... conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids containing other ...silencing complex (RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining...remaining single-stranded siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC... bypass the Dicer cleavage step and proceed to binding of RISC. For more information on shRNA design and..., 136 (4), 642–655. PubMed (Link opens in a new window) . Dana, H., Chalbatani, G. M., Mahmoodzadeh, H...Science, 13 (2), 48–57. PubMed (Link opens in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., ...

... in a new window) The Malate Dehydrogenase CUREs Community (MCC) (Link opens in a new window) develops...mentor future MCC members. Find a regional hub (Link opens in a new window) and contact the hub director...opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources...expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned... cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present ...plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human.... DOI: 10.24918/cs.2021.43 (Link opens in a new window) External Collaboration Results in Student Learning...

...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...directly after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded...CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in...protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy the...foreign DNA. Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...(tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9...

...origins should be used. Find further details in Mohler et al., Mol Syst Biol 2023 and find general tips on phosphoprotein...-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry fragment... fragment. Upon interaction between the phosphobinding domain and the phosphosite, mCherry fluorescence... #2 phosphosite library with four different phosphobinding domains (14-3-3β, 14-3-3σ, the WW2 domain of...s41592-022-01638-5. PubMed (Link opens in a new window) rEcoli XpS and a new pSerOTS described in: System-wide...10.15252/msb.202110591. PubMed (Link opens in a new window) Protocols for using iSPI, rEcoli XPS, and pSerOTS-C1... Aug;36(7):638-644. PubMed (Link opens in a new window) Kinase Substrate Profiling Using a Proteome-wide...

...collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell (hiPSC...Telomeric repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP AICSDP-144 mEGFP CCCTC-binding factor (CTCF...in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window) References... new window) PubMed All Allen Institute for Cell Science Publications (Link opens in a new window)...Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell line information from the Allen ...for Cell Science data portal (Link opens in a new window) , including: data and images from the hiPS cell...Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding protein FUS Paraspeckles/stress granules 114411...

...Plantae (Link opens in a new window) . TraVa (Link opens in a new window) - Database of gene expression...Addgene's plant resources: Find plasmids for use in plants. Plasmid...and a variety of other species. In addition to individual plasmids, Addgene carries several useful toolkits... rice. Includes a protocol (Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang...Biological Resource Center (ABRC) (Link opens in a new window) - The ABRC collects, preserves, reproduces, and...Information Resource (TAIR) (Link opens in a new window) - TAIR includes a database of genetic and molecular...Resource for Plant Biology (Link opens in a new window) - Tools for gene and protein expression and mapping...

... new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window) National...(Link opens in a new window) NIH AIDS Reagent Program (Link opens in a new window) Saccharomyces Genome...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with, or...they cause. The standard format for providing individual plasmids is a bacterial stab (of a laboratory...expression, and synthetic biology. CRISPR Resources : Find essential background information on using CRISPR...for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB plasmid set : E. ...