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We narrowed to 1,149 results for: IND

Showing: 41 - 60 of 1149 results

  1. COVID-19 Resources

    Type
    Collection
    ...available to industry, in addition to academics and nonprofits, and this is denoted in the industry column ...our Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene resources... SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI Additional...plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding nucleic acids with SHERLOCK and DETECTR...DETECTR (Link opens in a new window) SHERLOCK (Link opens in a new window) Addgene's Protocol for RNA ...a new window) COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv (Link opens in a new window) Elsevier's...opens in a new window) The Lancet COVID-19 Resource Centre (Link opens in a new window) The New England...

  2. Antibody Guide

    Type
    Collection
    ...structure are responsible for binding the antigen, and are called the antigen binding fragments (Fab). The tips...recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on...single individual, giving a high probability that an antibody will be able to recognize and bind to any... and binds to the protein of interest. Signal strength is determined by how many antibodies bind the protein...detection antibody is added and binds to the bound protein. If using the indirect sandwich method, a conjugated...? Will you be using a direct or indirect detection method? If indirect, do you already have secondary ...tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as part ...

  3. CRISPR References and Information

    Type
    Collection
    ... in a new window) . For more details, see Clement et al. (2019) (Link opens in a new window) . MAGeCK ...(Link opens in a new window) . gRNA Design DeepSpCas9 (Link opens in a new window) A webtool that uses...(Link opens in a new window) . Also try CRISPOR batch (Link opens in a new window) for lentiviral screens...the National Cancer Institute. Cas-OFFinder (Link opens in a new window) Identifies gRNA target sequences...Mateo (Link opens in a new window) Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA... in a new window) Labs. Off-Spotter: tool for CRISPR/Cas design (Link opens in a new window) Program for...opens in a new window) for more information. CRISPR MultiTargeter (Link opens in a new window) Can be used...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...Acinar Cells induced Beta Cells Adenoviral Mouse Long-term persistence and development of induced pancreatic... Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells (iPSCs) in both mouse and...expression of key factors. The tables below will help you find plasmids available from Addgene that can be used...search the iPSC Neurodegenerative Disease Initiative (iNDI) Collection for plasmids that can be used to create...Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the table ...species, or PI and then select the article link to find more information. Delivery Method Species Description...and Lin28 from four separate lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human ...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...a new window) Brain Banks (Link opens in a new window) Rodent Models (Link opens in a new window) Michael...reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant...Foundation (Link opens in a new window) A foundation dedicated to finding a cure for Parkinson's disease... opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ... Addgene has assembled the following to help you find information and plasmid collections that may help...triplet series within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin...

  6. CRISPR Guide

    Type
    Collection
    .... gRNA binding induces a conformational change in Cas9, shifting it into an active, DNA-binding configuration...( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome ...composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines... otif (PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends...with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases...undergoes a second conformational change upon target binding. In this formation, its nuclease domains, RuvC ...causes small nucleotide insertions or deletions (indels) at the DSB site. Therefore, a population of cells...

  7. Promega Plasmid Collection

    Type
    Collection
    ...collection (Link opens in a new window) on Promega.com. ID Plasmid Description Industry Plasmid Technology Overview...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from ...opens in a new window) from Promega Read about HaloTag technology (Link opens in a new window) from Promega... in a new window) from Promega Check out the Promega Connections (Link opens in a new window) blogs for...assays (Link opens in a new window) and NanoBRET assays (Link opens in a new window) NanoLuc®, HaloTag®, NanoBiT...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...Overview Additional Resources (Link opens in a new window) Explore cutting-edge research tools from Promega...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression...expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression...Mammalian, Varies Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids...for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression vector URA3...fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable marker. They are... plasmids for mammalian expression, Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian... plasmids for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression...

  9. p53 Pathway

    Type
    Collection
    ... increase its stability and DNA binding activity, and allow it to bind transcriptional co-activators and...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific...and DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like growth factor binding protein 3 KAI...protein p53 regulated apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B ...apoptosis effector PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and...these mutations are missense mutations in the DNA-binding domain, often at one of six hotspot residues. These... cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene...

  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Standard Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT...experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of novel ...plasmids. Online spreadsheet (Link opens in a new window) of Flp-recombinase-expressing transgenic mouse... maintains a public folder (Link opens in a new window) of manuscripts that have used these constructs...inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene...Methods 11(7):763-72. PubMed (Link opens in a new window) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, ...

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ...ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2, RAET1H ULBP3 UL16 binding protein...kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein...RING11 TAPBP TAP binding protein (tapasin) NGS17, TAPA, TPN, TPSN, tapasin TAPBPL TAP binding protein-like...factor beta binding protein 1 MGC163161 LTBP2 latent transforming growth factor beta binding protein 2 ... RAB GTPase binding effector protein 1 RAB5EP, RABPT5 RABEP2 rabaptin, RAB GTPase binding effector protein...cell is primed to recognize a specific antigen, it finds and attacks pathogens or infected cells expressing...T cell, whereas T cell tolerance or anergy are induced when the TCR is engaged without co-stimulation ...

  12. Fluorescent Proteins: FRET

    Type
    Collection
    ...s16091488 (Link opens in a new window) PMID: 27649177 (Link opens in a new window) Algar, W. R., Hildebrandt...0530-8 (Link opens in a new window) PMID: 31471616 (Link opens in a new window) Kappel, C. Kuschel, L. DeRose... in a new window) External Resources FPbase.org FRET calculator (Link opens in a new window) Nikon MicroscopyU...Link opens in a new window) Fluorescent Biosensor Database (Link opens in a new window) designed by Eric...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes..., scientists can use FRET to report on protein binding or conformational changes. Organic dyes, fluorescent...biosensors? Check out our Biosensors Collection to find fluorescent biosensors targeting metal ions, small...

  13. Institute for Protein Innovation

    Type
    Collection
    ...the ligand-binding pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific ...antibodies that uniquely: Bind to the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit... opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...Protein Innovation (IPI) Collection! Here, you’ll find unique recombinant antibodies targeting families...model. Learn more about IPI (Link opens in a new window) Glypican Antibodies Glypicans are a family of ...specific subunit pairs, blocking ligand binding and receptor function Figure courtesy of IPI Browse Integrin ...

  14. Mammalian RNAi Tools

    Type
    Collection
    ...enzyme, binds the shRNA and cleaves it to produce siRNA. The siRNA then binds the RNA-induced silencing... conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids containing other ...silencing complex (RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining...remaining single-stranded siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC... bypass the Dicer cleavage step and proceed to binding of RISC. For more information on shRNA design and..., 136 (4), 642–655. PubMed (Link opens in a new window) . Dana, H., Chalbatani, G. M., Mahmoodzadeh, H...Science, 13 (2), 48–57. PubMed (Link opens in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., ...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...your gene of interest. Inducible: The recombinase may be fused to a ligand-binding domain such as the human...buttons to find plasmids based on your expression system of choice, promoter, or search by inducible system...modify the genome or control gene expression, and find plasmids for your experiments. Plasmid...organisms. Each recombinase enzyme recognizes and binds pairs of short, specific DNA target sites, and catalyzes...or locations using cell-specific promoters or inducible systems, you can precisely control and optimize...tamoxifen. A tetracycline-regulated or other drug-inducible approach may also be used. Split proteins: The...recombinase systems can be combined in multiple, independent recombination events in a single experimental...

  16. Bacterial Expression Systems

    Type
    Collection
    ...you to independently control the expression of up to twelve genes using small-molecule inducers in the...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...curated collection of bacterial expression plasmids to find out more! Protein Purification To study the function...Resources Visit our Fluorescent Protein Guide to find an extensive collection of FPs, including photoactivatable...a certain distance. You can test if two proteins bind or interact together by fusing each of them to two...FP can get together and reconstruct the full FP. Find plasmids containing FPs and split FPs that can be...different bacterial systems. ID Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO...

  17. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...origins should be used. Find further details in Mohler et al. (2023) and find general tips on phosphoprotein... tRNA^Sep with anticodon, CUA. Engineered EF-Tu binds the phosphoserine-charged tRNA (AUC) and is brought...-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry fragment... fragment. Upon interaction between the phosphobinding domain and the phosphosite, mCherry fluorescence...phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the WW2 domain of...022-01638-5. PMID 36280721 (Link opens in a new window) rEcoli XpS and a new pSerOTS described in: Mohler...msb.202110591. PMID 37477096 (Link opens in a new window) Protocols for using iSPI, rEcoli XPS, and pSerOTS-C1...

  18. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... in a new window) The Malate Dehydrogenase CUREs Community (MCC) (Link opens in a new window) develops...mentor future MCC members. Find a regional hub (Link opens in a new window) and contact the hub director...opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources...expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned... cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present ...plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human.... DOI: 10.24918/cs.2021.43 (Link opens in a new window) External Collaboration Results in Student Learning...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...directly after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded...CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in...protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy the...foreign DNA. Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...(tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9...

  20. E11 Bio PRISM Collection

    Type
    Collection
    ... a new window) , in collaboration with The Francis Crick Institute (Link opens in a new window) , MIT ...new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window) . ...molecular tools for high-throughput reconstruction of individual neurons in brain tissue developed by scientists...tools enabling high-throughput reconstruction of individual neurons in brain tissue. PRISM begins with the... PRISM is part of an integrated pipeline, but individual components can be used separately for additional...also developed a set of Cre-dependent, hSyn-Tet inducible plasmids (Addgene #242782–242799) suitable for...Potential applications could also include using individual or subsets of barcode proteins to label and detect...
Showing: 41 - 60 of 1149 results