We narrowed to 1,093 results for: IND

...the ribosome binding site is inaccessible. When the target RNA is present, however, it binds to the switch...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...the dsRNA leading to increased RNAi efficiency. Find the T444T plasmid here! Strum A, et al. Nucleic ...an option, including Drosophila and C. elegans.   Find the TurboID plasmids here! Branon et al. Nat Biotechnol...switch and exposes its ribosome binding site, allowing for the gene on the toehold switch to be expressed...display and deep sequencing, revealing that they bind to a much larger subset of protein motifs than previously...biophysical study involving protein stability or binding experiments. Potential experiments that one might...

...fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator with an expanded...protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator applicable to optogenetics...Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium Indicators. Janelia...calcium indicators for optical imaging (GECO1, GECO1.2), including green, blue, and red indicators An Expanded...fluorescent calcium ion indicator OCaMP A sensitive orange fluorescent calcium ion indicator for imaging neural...Calcium Near-infrared indicator iGECI A near-infrared genetically encoded calcium indicator for in vivo imaging...calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium indicator enables...

...available to industry, in addition to academics and nonprofits, and this is denoted in the industry column ...our Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene resources... SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI Additional...plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding nucleic acids with SHERLOCK and DETECTR...DETECTR (Link opens in a new window) SHERLOCK (Link opens in a new window) Addgene's Protocol for RNA ...a new window) COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv (Link opens in a new window) Elsevier's...opens in a new window) The Lancet COVID-19 Resource Centre (Link opens in a new window) The New England...

...structure are responsible for binding the antigen, and are called the antigen binding fragments (Fab). The tips...recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on...single individual, giving a high probability that an antibody will be able to recognize and bind to any... and binds to the protein of interest. Signal strength is determined by how many antibodies bind the protein...detection antibody is added and binds to the bound protein. If using the indirect sandwich method, a conjugated...? Will you be using a direct or indirect detection method? If indirect, do you already have secondary ...tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as part ...

... in a new window) . For more details, see Clement et al. (2019) (Link opens in a new window) . MAGeCK ...(Link opens in a new window) . gRNA Design DeepSpCas9 (Link opens in a new window) A webtool that uses...(Link opens in a new window) . Also try CRISPOR batch (Link opens in a new window) for lentiviral screens...the National Cancer Institute. Cas-OFFinder (Link opens in a new window) Identifies gRNA target sequences...Mateo (Link opens in a new window) Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA... in a new window) Labs. Off-Spotter: tool for CRISPR/Cas design (Link opens in a new window) Program for...opens in a new window) for more information. CRISPR MultiTargeter (Link opens in a new window) Can be used...

...Acinar Cells induced Beta Cells Adenoviral Mouse Long-term persistence and development of induced pancreatic... Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells (iPSCs) in both mouse and...expression of key factors. The tables below will help you find plasmids available from Addgene that can be used...search the iPSC Neurodegenerative Disease Initiative (iNDI) Collection for plasmids that can be used to create...Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the table ...species, or PI and then select the article link to find more information. Delivery Method Species Description...and Lin28 from four separate lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human ...

...a new window) Brain Banks (Link opens in a new window) Rodent Models (Link opens in a new window) Michael...Foundation (Link opens in a new window) A foundation dedicated to finding a cure for Parkinson's disease... opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...Find materials useful for neurodegeneration research. Neurodegeneration... has assembled the following to help you find information and plasmid collections that may help with your...triplet series within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin...Presenilin 2, and APP. The majority (> 90%) of individuals develop late-onset AD. There is no clear association...

.... gRNA binding induces a conformational change in Cas9, shifting it into an active, DNA-binding configuration...( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome ...composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines... otif (PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends...with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases...undergoes a second conformational change upon target binding. In this formation, its nuclease domains, RuvC ...causes small nucleotide insertions or deletions (indels) at the DSB site. Therefore, a population of cells...

...collection (Link opens in a new window) on Promega.com. ID Plasmid Description Industry Plasmid Technology Overview...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from ...opens in a new window) from Promega Read about HaloTag technology (Link opens in a new window) from Promega... in a new window) from Promega Check out the Promega Connections (Link opens in a new window) blogs for...assays (Link opens in a new window) and NanoBRET assays (Link opens in a new window) NanoLuc®, HaloTag®, NanoBiT...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...Overview Additional Resources (Link opens in a new window) Explore cutting-edge research tools from Promega...

...the ligand-binding pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific ...antibodies that uniquely: Bind to the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit... new window) Welcome to the Institute for Protein Innovation (IPI) Collection! Here, you’ll find unique... opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...ligand binding and receptor function Figure 3: IPI Integrin antibodies exhibit distinct binding specificities... are used to estimate nonspecific binding due to Fc receptor binding or other protein-protein interaction...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...

...pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression...expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression...Mammalian, Varies Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids...for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression vector URA3...fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable marker. They are... plasmids for mammalian expression, Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian... plasmids for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression...

...Link opens in a new window) is a public-private partnership of 13 members from industry and academia financed...00109-1 (Link opens in a new window) PMID: 40355756 (Link opens in a new window) Goldmann, U., Wiedmer, T...00108-2 (Link opens in a new window) PMID: 40355757 (Link opens in a new window) Superti-Furga, G., Lackner...00056-6 (Link opens in a new window) PMID: 32265506 (Link opens in a new window) Wiedmer, T., Teoh, S.T.,...00106-4 (Link opens in a new window) PMID: 40355754 (Link opens in a new window) Wolf, G., Leippe, P., Onstein...00105-5 (Link opens in a new window) PMID: 40355755 (Link opens in a new window) Credits Contributing Authors...-optimized ORF sequences for human SLCs and SLC binder sequences in Gateway-compatible cloning vectors...

... increase its stability and DNA binding activity, and allow it to bind transcriptional co-activators and...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific...and DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like growth factor binding protein 3 KAI...protein p53 regulated apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B ...apoptosis effector PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and...these mutations are missense mutations in the DNA-binding domain, often at one of six hotspot residues. These... cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene...

...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Standard Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT...experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of novel ...plasmids. Online spreadsheet (Link opens in a new window) of Flp-recombinase-expressing transgenic mouse... maintains a public folder (Link opens in a new window) of manuscripts that have used these constructs...inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene...Methods 11(7):763-72. PubMed (Link opens in a new window) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, ...

...ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2, RAET1H ULBP3 UL16 binding protein...kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein...RING11 TAPBP TAP binding protein (tapasin) NGS17, TAPA, TPN, TPSN, tapasin TAPBPL TAP binding protein-like...factor beta binding protein 1 MGC163161 LTBP2 latent transforming growth factor beta binding protein 2 ... RAB GTPase binding effector protein 1 RAB5EP, RABPT5 RABEP2 rabaptin, RAB GTPase binding effector protein...cell is primed to recognize a specific antigen, it finds and attacks pathogens or infected cells expressing...T cell, whereas T cell tolerance or anergy are induced when the TCR is engaged without co-stimulation ...

...s16091488 (Link opens in a new window) PMID: 27649177 (Link opens in a new window) Algar, W. R., Hildebrandt...0530-8 (Link opens in a new window) PMID: 31471616 (Link opens in a new window) Kappel, C. Kuschel, L. DeRose... in a new window) External Resources FPbase.org FRET calculator (Link opens in a new window) Nikon MicroscopyU...Link opens in a new window) Fluorescent Biosensor Database (Link opens in a new window) designed by Eric...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes..., scientists can use FRET to report on protein binding or conformational changes. Organic dyes, fluorescent...biosensors? Check out our Biosensors Collection to find fluorescent reporters for metal ions, small molecules...

...enzyme, binds the shRNA and cleaves it to produce siRNA. The siRNA then binds the RNA-induced silencing... conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids containing other ...silencing complex (RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining...remaining single-stranded siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC... bypass the Dicer cleavage step and proceed to binding of RISC. For more information on shRNA design and..., 136 (4), 642–655. PubMed (Link opens in a new window) . Dana, H., Chalbatani, G. M., Mahmoodzadeh, H...Science, 13 (2), 48–57. PubMed (Link opens in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., ...

...your gene of interest. Inducible: The recombinase may be fused to a ligand-binding domain such as the human...buttons to find plasmids based on your expression system of choice, promoter, or search by inducible system...modify the genome or control gene expression, and find plasmids for your experiments. Plasmid...organisms. Each recombinase enzyme recognizes and binds pairs of short, specific DNA target sites, and catalyzes...or locations using cell-specific promoters or inducible systems, you can precisely control and optimize...tamoxifen. A tetracycline-regulated or other drug-inducible approach may also be used. Split proteins: The...recombinase systems can be combined in multiple, independent recombination events in a single experimental...

...Plantae (Link opens in a new window) . TraVa (Link opens in a new window) — Database of gene expression...and a variety of other species. In addition to individual plasmids, Addgene carries several useful toolkits...for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and fluorescent plasma membrane and...rice . Includes a protocol (Link opens in a new window) for targeted gene mutation. Combined fluorescent...dicot plants. Plant gene editing through de novo induction of meristems . A comprehensive multi-purpose toolkit...expressed from constitutive and arbuscular mycorrhiza-inducible promoters to label organelles, membranes and cytoskeletal...

...additional variants. David Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based) for inducible knockdown,...Benoist and Diane Mathis 21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin selection. ...promoter. Didier Trono 11651 pLVUT-tTR-KRAB 3rd Inducible expression of transgene or shRNA. See Szulc et...Gateway destination plasmid with constitutive or inducible expression of cDNAs, shRNAs or miRNAs, and use... Ie-Ming Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway plasmid, and...additional variants. Iain Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression...