We narrowed to 810 results for: MAL

...Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisomes Peroximal targeting signal (SKL) mCherry* Michael Davidson...Davidson 36203 pmTurquoise2-Peroxi Peroxisomes Peroximal targeting signal (SRL) mTurquoise2 Dorus Gadella ... Misteli 137806 mNeonGreen-peroxi Peroxisomes Peroximal targeting signal (SKL) mNeonGreen Dorus Gadella...Gadella 85063 pmScarlet_peroxisome_C1 Peroxisomes Peroximal targeting signal (SRL) mScarlet Dorus Gadella ...Gadella 189769 pmScarlet3-Peroxi_C1 Peroxisomes Peroximal targeting signal (SRL) mScarlet3 Dorus Gadella 61202...61202 pCMU-PERr Peroxisomes (Plants) Peroximal targeting signal (SKL) mCherry Maria Harrison 55146 mCherry-TOMM20...responsible for forming connections and adhesions between animal cells. They are important for holding cell structures...

...bind to a small molecule, the reporter plasmid can be used to detect the presence of that small molecule...Signal peptides for periplasmic localization: PelB, MalE, OmpA ID Plasmid Promoter Tags PI Additional Addgene...visualization inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent... Fluorescent Protein Guide for a selection of mammalian and bacterial FRET-related vectors and standards...expression levels can be controlled by a variety of small molecules, light, and temperature, in different ...control the expression of up to twelve genes using small-molecule inducers in the same E. coli strain. Addgene...

...Includes a wide range of reference genomes, including animals, plants, bacteria, fungi, protists, and viruses...Link opens in a new window) Program for designing optimal gRNAs. Provides feedback on number of potential...human. Developed by the George Church Lab . CRISPR Optimal Target Finder (Link opens in a new window) Identification...cloning pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor...Orkin and Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott...

...GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular cells...GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4 - AAV.CAP-B10...with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications ...

...BiT (LgBiT; 18 kDa) subunit and a small complementary peptide, Small BiT (SmBiT; 11 amino acids). This... and extracellular applications where you want minimal disruption of endogenous protein expression and...protein expression systems including E. coli , mammalian cells, and cell-free systems. NanoBiT Assays NanoLuc...association, and dissociation. HiBiT Fusions HiBiT is a small, multifunctional epitope tag enabling precise protein...

...fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector Hillel Adesnik 107708...channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by...export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome...genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708-AAV9 pAAV-hSynapsin1-RdLight1...genetically encoded dopamine sensor RdLight1 in mammalian neurons. Lin Tian 127090-PHPeB pAAV-CAG-DIO-ChR2... in AAV production vector expressed under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1...AAV production vector under the control of the mammalian promoter (EF1a) Francois St-Pierre 179463-AAV9...

... that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does... RNA Targeting CRISPR Plasmids: RNA Targeting Mammalian Bacteria Plant Resources Type VI CRISPR systems... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

... CRISPR Base Edit CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes...and they can convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently...editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing systems... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

...nuclease system to facilitate genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ....Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9... are two sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene ...adeno-associated virus (AAV). The single vector system uses a smaller Cas9 from S. aureus (SaCas9) that has been human...In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich...

... available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...CRISPR RNA Editing CRISPR Plasmids: RNA Editing Mammalian Resources Type VI CRISPR systems, including the...truncation retain RNA editing capabilities but are small enough to be packaged in AAV particles. Figure 1... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert PI Publication Return to...

...tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell...Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert... Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral Human REST suppression...

...conformational changes within a protein. Biosensors Monitor small biomolecules or other physiological intracellular... study molecular processes in organs and whole animals. Additional Resources at Addgene Optogenetics Guide...Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to activate or inhibit engineered receptors...

...delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...in-stock AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...

... CRISPR Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases... used in bacteria genome editing, though some mammalian systems have been developed. Figure 1: Overview... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

...cells, but imaging in deep-tissue organs or whole animals presents unique challenges. Genetically-encoded...proteins most suitable for stable imaging in live animals are those with emission near or above 650 nm (far-red...spatially selective imaging of tissues in living animals. Learn more about in vivo imaging tools in this...

...engineered receptors that are activated by specific small molecules, allowing a scientist to control signaling...engineered to respond to specific physiologically inert small molecules. There are four types DREADDs in Addgene's...receptor (nAChR) domains that respond specifically to small molecules termed Pharmacologically Selective Effector...

...for use in Drosophila . Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR...plasmids for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish...

...inducible expression of GFP T2A Cre fusion in mammalian cells. Floris Foijer 47916 pRXTN CMV/MSV A modified...-Luc MoMSV Stable expression of luciferase in mammalian cells. Alice Wong 83356 pMXs-3XHA-EGFP-OMP25 MoMSV... For tagging mitochondria with HA epitopes in mammalian cells. David Sabatini Return to top Popular Gamma-Retroviral...

...destination plasmid for cDNA expression. Miguel Ramalho-Santos 24129 pULTRA 3rd Bicistronic expression ...site in the 3´-LTR). Find more pULTRA versions . Malcolm Moore 25895 pLX301 3rd Gateway plasmid for CMV ...Kaufman 102930 pLJC5-Tmem192-3xHA 3rd Contains a lysosomal tag. See Abu-Remaileh et al. (2017) for additional...