...variation affects genome function to influence phenotypes....characterize and map genome function, how genome function shapes phenotype, and how these processes are affected...impact of genomic variants on genome function and phenotypes. Plasmids The collection will grow as the IGVF...

... use as a set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering ...here to view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A ...here to view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

...different types of proteins. For a full description (including peak response spectra) of different types of ...track its expression and localization. Many other types of proteins can also be controlled by light, generally...

... that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...plasmids to be packaged in their choice of five serotypes, resulting in over 15,000 possible choices! By...only package plasmids in our collection in the serotypes listed here. Details Scale and Titer Packaged ...

...in your target cells. For easy-to-transfect cell types (e.g. HEK293 cells), transfection with standard ...cell lines Useful for difficult to transfect cell types, can also be used in vivo A common choice for conducting...edit. CRISPR editing produces several possible genotypes within the resulting cell population. Some cells...

...ready-to-use viral preparations in engineered systemic serotypes from the Viviana Gradinaru laboratory and the .... To review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech...Boyden Citation Information When using the Caltech serotypes in future publications, please acknowledge Viviana...

...rearranged. The schematic below shows the three types of rearrangements: inversion, deletion and translocation...actin Xenopus Ryffel 32144 pJFRC170-3XUAS-IVS-Cre::PEST Cre expressed at low levels to reduce toxicity; ...activate or repress gene expression. Common construct types include: Cre-dependent Gene Expression: Placing ...orientation specificity of Cre and the different types of target sites available, both mutant and wild ...

...vectors in the indicated serotypes are no longer available as aliquots. Other serotypes may be available, click...Viral Vectors (Plasmids still available, other serotypes may be available) Based on low demand, production...

...found on almost any centrifuge. Types and Parts There are several types of centrifuges: Mini or Tabletop...tube. You will likely encounter several different types of centrifuges that can accommodate different sized... are typically lower than some other centrifuge types. Floor Centrifuges sit on the floor and can be about...

...complex 3, beta 1 subunit ADTB3, ADTB3A, HPS, HPS2, PE AZGP1 alpha-2-glycoprotein 1, zinc-binding ZA2G, ... can be secreted by immune cells and other cell types. They can be grouped in to the CC, CXC, XC, and ...response through effects on numerous immune cell types including T cells and B cells. It also impacts the...motif) ligand 27 ALP, CTACK, CTAK, ESKINE, ILC, PESKY, SCYA27 CCL28 chemokine (C-C motif) ligand 28 CCK1...necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) ATAR, HVEA, HVEM, LIGHTR, TR2...

...designed to express nucleases in different cell types. Zinc finger arrays can be transferred in-frame ...designed to express nucleases in different cell types. Zinc finger arrays created using ZFC reagents for...

...physiologically inert small molecules. There are four types DREADDs in Addgene’s collection: hM3D(Gq), rM3D(...pore domain it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are activating, and...

...reducing its infective potential. The following types of viral particles have been pseudotyped with the...firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral vector expressing destabilized...

...information on each gene, and examples of plasmid types available for each gene. Click the gene name for...

...and in vivo to use in biosensors. The most common types of luciferase found in Addgene’s collection are ...transcriptional regulatory region in specific cell types. Used to identify functional SNPs from loci determined...

...differential chromatin accessibility across different cell types and cloned into an AAV backbone. Most enhancers ...mouse lines for genetic access to cortical cell types. bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244...

... I.1 Sequence of pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-...particles can efficiently infect a broad range of cell types, including both dividing and non-dividing cells....stuffer insert is shown in all capital letters. I.2. Recipes Luria Broth Agar (LB agar) + antibiotic Per 40 ...

...independently of DNA synthesis. McCarty DM, Monahan PE, Samulski RJ. Gene Ther. 2001 Aug;8(16):1248-54. ...cells and is a key advantage of rAAV. AAV Serotypes Eleven serotypes of AAV have thus far been identified,...commonly used being AAV2. These serotypes differ in their tropism, or the types of cells they infect, making...transducing specific cell types. The chart below gives a summary of the tropism of AAV serotypes, indicating the...capsid and genome from different viral serotypes. These serotypes are denoted using a slash, so that AAV2...Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you ...you about AAV components, serotypes, pseudotyping, and more. Science...

...RNA-binding protein to stabilize pegRNAs Twin PE - two PEs make complementary edits to opposite strands...stability PEmax - optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced... Overview of prime editing Popular prime editing (PE) tools include: PE2 - improved RT enzyme efficiency...employed to knock out target genes in various cell types and organisms, but modifications to various Cas ...are rare cases where Acr can work with multiple types, such as AcrVA3.1, which inhibits both Cas12a and...about 8 bp, specified by a gRNA. There are two main types of base edits: Base transitions - edit to same classification...then carries out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration...

...or to differentiate human iPSCs into other cell types, as noted in the purpose field: ID Plasmid Description...