Custom CRISPR Screens & the Green Listed Software
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Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...Photoactivatable FPs in Empty Backbones in the Michael Davidson Collection Find Photoactivatable FPs for Single... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...ultraviolet (UV) light, they do so via aromatic amino acids found in all proteins, so you can't distinguish ... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...Additional resources on Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our... -
Technical Design of a Western Blot
TypeBlog Post...recombinant antibodies (rAbs), which are produced from plasmids and have very little batch-to-batch variation.... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...modifications Check literature Highly charged amino acids Run control protein (if available) and note in... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid...elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid...Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid...Selectable Marker PI Publication Return to top Parasite ID Plasmid Gene/Insert Promoter Selectable Marker PI...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...CRISPR plasmids that create double strand breaks in DNA. CRISPR... -
CRISPR Plasmids - Mammalian Expression
TypeCollection... NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...diverse populations of mutants for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI... and are potentially heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid... top Drosophila ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert... top C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Activate CRISPR Plasmids: Activate Mammalian Bacteria Drosophila Plant C. elegans Yeast Resources Catalytically... search the tables below for CRISPR activation plasmids. To learn more about CRISPR activation and other... -
CRISPR Plasmids - Bacteria
TypeCollection...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated... A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting... -
AAV Molecular Tools
TypeCollection...tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118... purification (which can be used for viral TRAP). ID Name Expression System Activity Serotype PI 98747...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509... encode tools that can be used for cell ablation. ID Name Expression System Activity Serotype PI 124364...the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System Activity Serotype PI 61592...tools that can be used for cell or protein labeling. ID Name Expression System Activity Serotype PI 149415...These AAV encode various genes for overexpression. ID Name Expression System Activity Serotype PI 58376... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid...Selectable Marker PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR... CRISPR Nick CRISPR Plasmids: Nick Mammalian... or search the tables below for CRISPR nickase plasmids. To learn more about CRISPR nickases and other... -
CRISPR Plasmids - Yeast
TypeCollection...paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI... A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable Marker PI... -
Serotype Testing AAV
TypeCollection...material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 37825-AAV1.T 20 ...material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 50465-AAV1.T 20 ...please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample is denoted with...with a .T suffix (for example, AAV1.T). The catalog ID for the 100 µL sized sample does not have the suffix...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need.... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid... Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...CRISPR plasmids for transcriptional repression of target genes. CRISPR... CRISPR Interfere CRISPR Plasmids: Interfere...search the tables below for CRISPR interference plasmids. To learn more about CRISPR interference and other...by organism by visiting our CRISPR plasmids page . Find a comprehensive list of CRISPR resources by visiting... -
CRISPR Plasmids - Base Edit
TypeCollection...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid... Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid...Selectable Marker PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR...CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors ...search the tables below for CRISPR base editing plasmids. To learn more about base editing and other CRISPR... -
CRISPR Plasmids - Plants
TypeCollection...paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication... A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI... -
Institute for Protein Innovation
TypeCollection...Antibody Plasmids The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid...Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity... epitope tags and other common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity...Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids Welcome to the Institute for Protein Innovation...) and common epitope tags, as well as antibody plasmids to create or express plasmid-based antibodies.... -
CRISPR Plasmids - Drosophila
TypeCollection...paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick ...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...