...fragments can be used in experiments such as immunoprecipitation and super-resolution microscopy. Two commonly...amber codons in scFvs A new expression vector facilitating production and functional analysis of scFv antibody...

...integrates these signals to determine the nutritional state of the cell and regulates the switch from anabolic...multiple familial cancers are caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation...

...name, capsid, and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table contains a general .... ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have suggestions for other plasmids...

...Depending on the pipette you’re using, you will be rotating dials behind the numerical display or the top ...

...incubator. Section 3: BCD Feed and valproic acid supplementation During the 24–144 h post-transfection, supplement...

...i.e., genome containing) AAV particles within representative vector preparations was quantified with electron...for viral production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids...

...cut the bottom of the Gravitrap column at the indentation in the tip. Equilibrate the column with 10 mL...

...Different SDS-PAGE gel tanks will have different gel orientations. Refer to the instructions for your specific...

... before any wells display a green product. A precipitated product indicates the presence of too much HRP...

...Sample Calculation First, use a hemocytometer to quantitate the homogenized cell solution. Homogenized cell...

...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...

...Rat Mouse IgG2a 140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest...83R] BAF53a Human Mouse IgG2a 206558 Anti-ATAT1 [N347/42R] ATAT1 Human Mouse IgG2a 206559 Anti-Foxi2 [N349...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...

...Next-Generation Sequencing (NGS) to confirm library representation. CRISPR screening experiments require large...Human Wei 3rd Varies 12,472 pairs HR700_TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe...

...Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana...Service . Addgene provides a viral service to help facilitate scientific research. Addgene's AAV are made from...

...Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...Plasmids - Stockholm Plasmid Gene/Insert Vector Type Mutations Tags Industry PI...

...Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

.... ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...search the table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools...

...Optogenetics The lab of Michael Davidson from Florida State University has contributed their comprehensive collection...

...distributes ready-to-use recombinant antibodies to help facilitate scientific research. We are building a collection...

... Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic...

...technologies also indirectly modify local chromatin state. Want more information on the wide variety of Cas...

...making it a very flexible editing system. The T375G mutation reduces off-target effects of ADAR2 by destabilizing...

...identification of potential RGN target sites and to facilitate design of short oligonucleotides needed to construct...

...RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless...

...CRISPR, researchers have expanded chromatin immunoprecipitation (ChIP) to allow purification of any genomic...

...and may not be suitable for applications where orientation matters. Type V-K systems use a type V-K Cas ...