... (blue light-inducible AraC dimers in E. coli) that turns expression on and off with blue light.   Inspired...domain with VVD, a light-responsive domain that dimerizes upon exposure to blue light (Fig. 1). Another...VenturaIf you follow Barbara Di Ventura on Twitter, you might have seen the video of her singing about the paper...Another blue light-inducible dimerization domain, Vaucheria frigida aureochrome1 (VfAu1), led to functional...most applications.   Figure 1: With blue light, the VVD domains of the BLADE dimerize and activates...under the pBAD promoter, can be controlled with light without the need of any cloning.     What advantages...BLADE activates transcription with the flick of a light switch and shuts off just as quickly. The original...

... focused on three different levels of assembly: High efficiency and accurate single-insert assembly Intermediate...destination plasmid. Antibiotic selection allowed for high throughput cloning efficiency measurements. We performed...confirmed the insertion of the Lambda insert at the same high frequencies as the antibiotic selection markers....lacZ cassette assembly was easily achievable with high levels of transformants and low backgrounds – so...Using these 4 bp overhang observations, accurate “high-fidelity” junction sets for both the 12- and 24-...complex assemblies were performed. It was found that high efficiencies and accurate assembly levels were indeed...in the number of transformants while maintaining high levels of correct assemblies) translate to faster...

...This is where FLEx enters the scene. FLEx: A light when night is about you! The FLEx (for flip-excision)...optogenetics? Optogenetics: Controlling neurons with light In 2005, a team from Stanford University, led by...ago with the discovery of microbial rhodopsins, light-sensitive proteins that absorb photons for energy...maintain membrane potential in response to visible light (Zhang et al., 2011). What do microbial rhodopsins...2000). Most neurons in the brain are not naturally light-sensitive, so the selective expression of opsin ...Optogenetic neuromodulation is made possible by the light: when hit with the correct wavelength, microbial..., 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins that, in turn, trigger...

...showed that improved specificity of Cas14 enables high-fidelity SNP genotyping. The development of  Cas14...

...Scientist. I used to spend my days (and often my nights :D) engineering E. coli to produce biofuels in ... screaming from the lab like the cartoon on the right?!? I would love to tell such a dramatic story, but...This may come as somewhat of surprise to many of my high school friends. In my teenage years I was super ...can involve walking alongside it and giving it a high five. While screaming can be therapeutic and I’ve...

...regulatory elements, pre-assembled genetic circuits, and higher level gene networks. Examples Include: promoters...

... NETRF believes that creating and maintaining a highly collaborative NET research community is pivotal...

...and Cpf1, which require that each DNA target have high sequence identity to the spacer sequence and contain...

...CRISPR-based purification methods are more straightforward and enable direct identification of molecules...

...resulting in frameshifts. If a repair template with high homology to the DNA surrounding the DSB is introduced...

...fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular signals...

...for Protein Innovation Collection : Antibodies to highly conserved proteins and other difficult-to-target...

... target various genes or genomic regions. It is highly recommended that you determine if your cell type...

...relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection of 20 CRISPR...

...FLASH (Fast Ligation-based Automatable Solid-phase High-throughput) assembly method (Reyon and Tsai et al...

... available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been ...

...Gly-Ser in-frame with prefix start codon; Employs high-efficiency enzymes. Prohibited Restriction Sites...

...hundreds of in-stock preps that are ready to ship right away, as well as our Packaged on Request service...

... in bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome...

... Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L....

... The p53 name arose from its observed molecular weight (53 kDa) when run on an SDS-PAGE gel. Cancer Impact...

...and Cpf1, which require that each DNA target have high sequence identity to the spacer sequence and contain...

...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...

...erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 ...

...is not compatible with other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing...

...transgene expression, contains intron that gives you high expression. Trono 12254 pWPI 2nd EF-1alpha driven...

...vector serves as a backbone to clone the left and right homology arms. More details on how to use this plasmid...

... pathways. CrispyCrunch CrispyCrunch is used for high-throughput design and analysis of CRISPR + HDR experiments...

...TALENs can be used to induce targeted mutations with high efficiency in zebrafish ( Sander et al., Nat Biotechnol...

...Looger 106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn Tight affinity glutamate sensor Glutamate sensor Looger...