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We narrowed to 1,052 results for: FER

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Showing: 61 - 80 of 1052 results

  1. Rett Syndrome

    Type
    Collection
    ... mutations in methyl-CpG-binding protein 2 confer different severity in Rett syndrome. Neurology . 70,...system for generating Rett syndrome-specific differentiated cells that can be used to understand the mechanisms...immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific... editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...microarray data, ISH image data, and anatomic reference atlases of prenatal and adult human brain (Link...(Link opens in a new window) Rettsyndrome.org References Amir et al. 1999. Rett syndrome is caused by ...MeCP2 levels display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... genomic engineering Luciferase Reporter pGL3 Basic Vector - Firefly luciferase vector for investigating... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...an insert using viral vectors, are studying a different genetic element, or want to express shRNA. Choose...investigating regions controlling transcription See our Luciferase collection of plasmid backbones into which you...target genes can be inserted into this firefly luciferase reporter to test for their effects on protein...lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG...

  3. Bacterial Expression Systems

    Type
    Collection
    ...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...replication in many gram-negative bacteria with different purification tag combinations, including PelB ... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...of small molecules, light, and temperature, in different bacterial systems. ID Plasmid Promoter Inducer...easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under the control of...FFluc Promoter activity Luminescence (firefly luciferase) Mycobacterium sp. Brian Robertson , Siouxsie...

  4. AAV Packaged on Request

    Type
    Collection
    ...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...handled at the lowest biosafety protection levels. We offer 1,077 in-stock viral vector preps, delivering high...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

  5. Retrograde AAV viral preps

    Type
    Collection
    ...Optogenetics Ofer Yizhar 125712 pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE CamKII Inhibitor Optogenetics Ofer Yizhar...retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations ...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...pAAV-CKIIa-stGtACR2-FusionRed CamKII Inhibitor Optogenetics Ofer Yizhar 105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed ...viral project team in collaboration with David Schaffer (University of California, Berkeley). Don’t See...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  6. Plant Plasmids and Resources

    Type
    Collection
    ...popular plasmids for gene editing in plants. Please refer to Addgene's Plant CRISPR Resources for a full selection... selection of plasmids expressing different functional variants of Cas9. ID Plasmid Description PI Genome...Resources for a selection of plasmids expressing different functional variants of Cas9. A CRISPR/Cas9 toolkit...the expression of microRNAs (miRNAs) or small interfering RNAs (siRNAs) to achieve gene silencing in plants...Complementation (rBiFC) and Förster Resonance Energy Transfer (FRET). Gateway Christopher Grefen 1000000222 .../Cas9 CRISPR-mediated Plant Base Editors RNA Interference in Plant Biology: New Tools for an Old Favorite...

  7. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...enzyme is present. Read on to learn about the different ways that Cre-lox and other site-specific recombinases...C-terminal fragments and placed under the control of different promoters. Expression of both fragments in the...the recombinase to align with your organism's preferred codon usage will improve expression. For example...of the orientation specificity of Cre and the different types of target sites available, both mutant and...such as Drosophila and mouse, particularly in proliferating cells. Expression may cause repetitive recombination...Use this AAV CRISPR knockout library expressing luciferase and Cre to interrogate tumorigenesis in vivo ...

  8. Biosensor AAV Preps

    Type
    Collection
    ...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 51083 AAV-EF1a-...-pA CAG GCaMP6f mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 51082 AAV-EF1a-...-pA CAG GCaMP6s mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 68720 pAAV-hSyn1...-pA Syn GCaMP6s mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  9. Chemogenetics Plasmids

    Type
    Collection
    ...channelrhodopsin. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element such ...neuron. LMO can be activated either by adding the luciferin substrate or by external illumination, making ...or other terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics...

  10. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...guaranteed here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...described here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...

  11. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Plasmids Supplemental Information New England Biolabs offers non-toxic protein labeling systems based on SNAP...constructs are compatible with multiple applications (different fluorophores, biotin, bead purification, blocking.... For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...described previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson...

  12. mTOR Pathway

    Type
    Collection
    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...Screening Kit (from David Sabatini & Kris Wood) References mTOR signaling in growth control and disease....

  13. Distribution to Industry

    Type
    Collection
    ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  14. Ras Pathway

    Type
    Collection
    ...migration, survival, adhesion, growth, and differentiation. Schematic of Ras signaling cascade that starts...ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential for regulating... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related...Initiative - at the National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam...

  15. Control AAV Preps

    Type
    Collection
    ... 5, 8 James M. Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 James M. Wilson...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8 AAV9... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Phosphoprotein Reagents Phosphoprotein Synthesis Reagents References & Protocols Additional Resources Figure 1: SepRS...performing phosphoproteomic experiments in need of reference spectra or phosphopeptide standards for quantitative...The Mode #2 phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the ...phosphorylation positional isomers are allocated to different subpools, allowing precise knowledge of phosphorylated... SepOTSα SepRS/EF-Sep ( aka. pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated...

  17. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...express nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors...plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC...either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

  18. Brain Initiative Collection

    Type
    Collection
    ... EGFP under control of human synapsin1 promotor. Ofer Yizhar 198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE Expresses... EGFP under control of human synapsin1 promotor. Ofer Yizhar 198516-AAV1 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 198516-AAV5 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 202199-AAV1 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...synapsin1 promotor after Cre-dependent recombination Ofer Yizhar 202199-AAV5 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...synapsin1 promotor after Cre-dependent recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use...

  19. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion... Viral Vector Packaging Service Addgene offers flexible packaging options so you can easily access... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...

  20. Optogenetics Plasmid Collection

    Type
    Collection
    ...spectra) of different types of opsins and optical switches available in these tables, refer to Addgene'...dimerization, localization or activity of many different types of proteins. For a full description (including...or other terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics...
Showing: 61 - 80 of 1052 results