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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,051 results for: FER

Showing: 61 - 80 of 1051 results

  1. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred from an excited...excited donor fluorophore to an acceptor. Since the transfer of energy does not occur by emission of a photon...FRET experiments. The efficiency of the energy transfer is measured using fluorescence microscopy by exciting...separately fused to a donor or acceptor molecule (also referred to as intermolecular or bimolecular FRET) or (...acceptor are both fused to the same protein (also referred to as intramolecular or unimolecular FRET). Fluorescent...fused between ECFP and Venus Back to Top FRET Reference Standards The following plasmids have well-characterized...

  2. Bacterial Expression Systems

    Type
    Collection
    ...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...replication in many gram-negative bacteria with different purification tag combinations, including PelB ... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...of small molecules, light, and temperature, in different bacterial systems. ID Plasmid Promoter Inducer...easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under the control of...FFluc Promoter activity Luminescence (firefly luciferase) Mycobacterium sp. Brian Robertson , Siouxsie...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection of plasmid backbones... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...an insert using viral vectors, are studying a different genetic element, or want to express shRNA. Choose...target genes can be inserted into this firefly luciferase reporter to test for their effects on protein...lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG...

  4. AAV Packaged on Request

    Type
    Collection
    ...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...handled at the lowest biosafety protection levels. We offer 1,001 in-stock viral vector preps, delivering high...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

  5. CRISPR Plasmids - Plants

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...pyogenes Bar Chen 50580 pBUN6I11 OsU3 BsaI yes, interfere S. pyogenes Bar Chen 50582 pBUN501 AtU6-26 BsaI...pyogenes Hyg Chen 50587 pHSN6I01 AtU6-26 BsaI yes, interfere S. pyogenes Hyg Chen 50589 pHSN501 AtU6-26 BsaI...

  6. Biosensor AAV Preps

    Type
    Collection
    ...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51083 AAV-EF1a-DIO-GCaMP6f-...GSG-P2A-GCaMP6f-WPRE-pA CAG GCaMP6f mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51082 AAV-EF1a-DIO-GCaMP6s-...GSG-P2A-GCaMP6s-WPRE-pA CAG GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 68720 pAAV-hSyn1-Flex-mRuby2...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  7. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...enzyme is present. Read on to learn about the different ways that Cre-lox and other site-specific recombinases...C-terminal fragments and placed under the control of different promoters. Expression of both fragments in the...the recombinase to align with your organism's preferred codon usage will improve expression. For example...of the orientation specificity of Cre and the different types of target sites available, both mutant and...such as Drosophila and mouse, particularly in proliferating cells. Expression may cause repetitive recombination...Use this AAV CRISPR knockout library expressing luciferase and Cre to interrogate tumorigenesis in vivo ...

  8. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion...options below to get started. In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock...in-stock preps? Our Packaged on Request service offers you even more options. Select from a vast catalog... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...

  9. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...guaranteed here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...described here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...

  10. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Plasmids Supplemental Information New England Biolabs offers non-toxic protein labeling systems based on SNAP...constructs are compatible with multiple applications (different fluorophores, biotin, bead purification, blocking.... For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...described previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson...

  11. mTOR Pathway

    Type
    Collection
    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...Screening Kit (from David Sabatini & Kris Wood) References mTOR signaling in growth control and disease....

  12. Distribution to Industry

    Type
    Collection
    ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  13. Control AAV Preps

    Type
    Collection
    ...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105534 ...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8 AAV9... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  14. Ras Pathway

    Type
    Collection
    ...migration, survival, adhesion, growth, and differentiation. Schematic of Ras signaling cascade that starts...ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential for regulating... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related...Initiative - at the National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam...

  15. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Phosphoprotein Reagents Phosphoprotein Synthesis Reagents References & Protocols Additional Resources Figure 1: SepRS...performing phosphoproteomic experiments in need of reference spectra or phosphopeptide standards for quantitative...The Mode #2 phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the ...phosphorylation positional isomers are allocated to different subpools, allowing precise knowledge of phosphorylated... SepOTSα SepRS/EF-Sep ( aka. pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated...

  16. COVID-19 Resources

    Type
    Collection
    ...spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids that have been...information on these genes, see the links and references below: ACE2 - (Human Angiotensin Converting Enzyme...Material. Rauch J, et al. bioRxiv 2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific...Addgene's Protocol for RNA Extraction Without A Kit References ACE2 - Andersen, KG, et al, 2020, Nature Medicine...2014, J Virol. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry...

  17. Brain Initiative Collection

    Type
    Collection
    ... EGFP under control of human synapsin1 promotor. Ofer Yizhar 198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE Expresses... EGFP under control of human synapsin1 promotor. Ofer Yizhar 198516-AAV1 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 198516-AAV5 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 202199-AAV1 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...synapsin1 promotor after Cre-dependent recombination Ofer Yizhar 202199-AAV5 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...synapsin1 promotor after Cre-dependent recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use...

  18. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... CRISPR Interfere CRISPR Plasmids: Interfere Browse CRISPR Plasmids By Function...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...

  19. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...express nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors...plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC...either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

  20. Chemogenetics Plasmids

    Type
    Collection
    ...opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making...or other terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics...
Showing: 61 - 80 of 1051 results