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Showing: 61 - 80 of 926 results

  2. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding the ...Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none...

  4. AAV Viral Preps

    Type
    Collection
    ...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...

  5. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  6. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  7. CRISPR Plasmids - Parasites

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids for neuronal excitation and inhibition. For example, search for "ChR mCherry", "Cre inhibition", "Boyden...to control a protein or cellular activity. For example, search "Cdc42", "transcription", "Hahn", or other...

  9. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...reducing the time to experiments by removing the amplification and extraction steps required when one receives...through our website. Every order will need to be completed independently including the check out and will...

  10. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Our production process and quality control, with examples of quality control data. Help Center Articles ...will also include virus-associated DNA, which is a sample of the purified DNA from the original plasmid that...

  11. AAV Molecular Tools

    Type
    Collection
    ...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's collection: HIV-based lentiviral...and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for measuring...

  13. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  14. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...

  15. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...allow for dual labeling and visualization of complementary protein function or localization. ACP and MCP...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...

  16. AAVED

    Type
    Collection
    ...information in the form of data and protocols showing examples of AAV strategies for intersectional control. ...information. Experimental protocols or schematics. Examples of research materials to share: In vivo data: ...

  17. Microbiology Resources

    Type
    Collection
    ...Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia...

  18. Qi Lab CRISPR Page

    Type
    Collection
    ...uide RNA (sgRNA) designed with a 20-basepair complementary region to any gene of interest. Co-expression...or PAM, sequence: NGG) juxtaposed to the DNA complementary region. The uniqueness of CRISPRi, as compared...

  19. Plant Plasmids and Resources

    Type
    Collection
    ...variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose...system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome editing logistics -...

  20. Open Enzyme Collection

    Type
    Collection
    ...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...
Showing: 61 - 80 of 926 results