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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 624 results for: Emb

Showing: 81 - 100 of 624 results

  1. Synthetic Biology - Bacterial

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  2. Synthetic Biology - Fungal

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  3. Synthetic Biology - Mammalian

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  4. Synthetic Biology - Plant

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  5. Synthetic Biology - Worm

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  6. Synthetic Biology - Yeast

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  7. Synthetic Biology - Strains

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    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...

  10. Synthetic Biology - Metabolism

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...

  13. MAPK Plasmids

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    Collection
    ...research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community. If you...

  15. CRISPR Plasmids - Cascade-Cas3

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    ...Design Tools CRISPR Blog Posts Cas3 is a unique member of the CRISPR family. After nicking the target ...

  16. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... your membrane, run a double-membrane transfer with a psi membrane stacked behind the p membrane. If your...through the p membrane into the psi membrane, that’s a good indication that the p membrane pores are too...marked on the membrane (1) using the gel as a guide. The gel is then removed from the membrane (2) and the...troubleshoot.   Before you start… Mark your membrane! Membranes look the same from both sides, so it’s quite...appropriately sensitive. If using a PVDF membrane, you can dry the membrane, wet it with 20% methanol, and place...completely through the membrane or larger proteins not transferring completely onto the membrane. If you’re not...completely through your membrane to the other side), you can check by using two membranes during your transfer...

  17. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... your gel and membrane transfer as normal. Immediately after transfer, dry the membrane to maximize protein...manufacturer’s instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed ... as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it to buffer, ...depend on your specific experiment. Remember, stripping your membrane will remove some of your sample —and...’ll want to: Rinse the membrane in water or fresh buffer. Cover the membrane in stripping solution and...a fume hood. Rinse the membrane in water or fresh buffer. Cover the membrane in stripping solution and...you need to start a whole new blot? Never fear: membrane stripping is here! Rather than start over, you...

  18. AAV Molecular Tools

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    Collection
    ...Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling...

  19. Human Germline Editing Using CRISPR

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    Blog Post
    ...off-target editing sites in the embryos, as well as whole exome sequencing of embryo derived-ES cells.They also...editing between iPS cells and embryos make a case for conducting research in embryos should our society decide...sequencing to a small percentage of edited embryos. Since the embryos produced were not being used to establish...-free embryos to only 60%? Unfortunately, given the differences between CRISPR repair in embryos and iPS...this paper. Any hint of CRISPR editing in human embryos has been met with a storm of media coverage. But... replication. Indeed, they observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since...MYBPC3 allele. Interestingly, they found that the embryos underwent repair not from the single-stranded oligonucleotide...

  20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...nitrocellulose membranes and exposed to IPTG in order to induce protein expression. Cells on the membranes will ...orientation of each membrane on its plate before moving on. Then peel each membrane off the plate (saving...high salt.  To probe membranes with target bacterial cells, wash your membranes with PBST to remove salt... on the membrane to bind their target cells, pulling them out of solution. Wash your membranes well with... 20 Petri dish-sized circles of nitrocellulose membrane and press each gently down onto a plate of colonies...regrow the lifted colonies overnight), flip the membrane over and place it colony-side up onto a fresh ...plasmid selection. Remove air bubbles between the membrane and the agar using forceps. Several protein induction...
Showing: 81 - 100 of 624 results