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...activity in a variety of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're Looking For? Please...within 1 business day regarding your account verification. Once your account has been verified, you will.... Pricing Addgene is a nonprofit organization dedicated to providing the scientific community with open...access to plasmid research tools. Pricing will be indicated on the product pages after you login to your account... the depositing institutions. These agreements indicate the language which we are allowed to use for distribution...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...Blood Cells. Stem Cells. 2012 Nov 29. Yamanaka Replicating EBNA1 episome Human Non-integrating EBNA1-mediated... Nat Methods. 2011 May;8(5):409-12. Yamanaka Replicating EBNA1 episome Human Non-integrating polycistronic... S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-...Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein Replicating EBNA1 episome Human Non-integrating EBNA1-mediated...RNA Human Non-integrating, polycistronic, self-replicating VEE RNA species expressing human Oct4, Klf4, ...generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013 Aug 1;13(2):246-54....Science. 2008 Nov 7;322(5903):949-53. Yamanaka Replicating EBNA1 episome Mouse Non-integrating EBNA1-mediated...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...the most abundant forms of posttranslational modification in cells and research into its many roles in...Importantly, phosphorylation positional isomers are allocated to different subpools, allowing precise knowledge...BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 34623 SepOTSα SepRS/EF-Sep ( aka...phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions ....programmable protein phosphorylation . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9130... using cell-free protein synthesis . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9168...in a new window) Additional Resources See our dedicated Genetic Code Expansion collection page Read about...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Selectable Marker PI Publication Return...integration of large fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead... (comprised of multiple components) to target locations in the genome defined by a guide RNA. The transposase...insertions — meaning they may not be suitable for applications where orientation of the insert is important...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation...number of potential off-targets, target's genomic location, and genome annotation. Available genomes are ...sequence, which can be an accession number, genomic location, pasted nucleotide sequence, or a sequence text... Target Finder (Link opens in a new window) Identification of gRNA target sequences from an input sequence...window) A collection of protocols, materials, and publications by members of the Division of Signaling and ...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ...CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system...

...dehydrogenase (MDH). The MCC is a national group of educational leaders providing research opportunities to their...other members of the MCC community. They support educators interested in integrating MDH-related research...professional development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor...of action, regulation and post-translational modifications, and metabolic pathways and protein interactions...His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present...access for biochemistry research in undergraduate education: The malate dehydrogenase CURE community. J Biol...

...Antibody Description Isotype Reactivity Recommended Applications PI Browse Glypican Antibodies Glypicans are ...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Synaptic Cleft IPI’s Synaptic...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Integrins Integrins are a family...Antibody Description Isotype Reactivity Recommended Applications Return to top Browse Epitope Tag Antibodies ...Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope tags and other...Antibody Description Isotype Reactivity Recommended Applications Return to top Browse Antibody Plasmids The following...

...recommended applications. The specific QC experiments performed varies for each antibody catalog item. Whenever...specific antibody can be found on the antibody's catalog page or by contacting [email protected] . Some of...Innovation (IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage....cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...aliquots before storing at -20 °C. Each antibody catalog item is tested for shipping stability by incubating...

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...antibodies, and Fc-silent mutants. Functional diversification of hybridoma-produced antibodies by CRISPR/...456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

...cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein and ...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis... below. Color is used for clarity and does not indicate a specific relationship. The content for this ...Pathway Color is used for clarity and does not indicate a specific relationship. The content for this ...Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG Regulatory...Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC PKC alpha...

... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...61592 : PX600; CMV-driven SaCas9 60957 : PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression...expressing human codon-optimized SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority... consists mainly of managing symptoms through medication and surgery. PD primarily involves the malfunction...available from Addgene, sortable by type of genetic modification and target species. You can also find our guide...synaptic transmission with improved glutamate indicators . (Aggarwal et al. bioRxiv. 2025) Monitor dopaminergic...window) A news website and information resource dedicated to helping researchers accelerate discovery and...Foundation (Link opens in a new window) A foundation dedicated to finding a cure for Parkinson's disease through...

... Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top...Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

...Genomic Tools for library construction, genomic modification, and cloning systems. Jump to Table: Cloning... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ... or sort by the table headings. Click on the publication link to view all other plasmids from that article...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...Additionally, we have developed a variety of educational resources to help you choose and use antibodies...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

...DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates...binding. Editors carrying the delta-984–1090 ADAR2 truncation retain RNA editing capabilities but are small... Guide . Mammalian ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

... for non-targeting control vectors? Search our catalog for control gRNAs . All-in-one Plasmids (gRNA and...Plasmid Vector Type Promoter Selectable Marker PI Publication Return to top gRNA-only Plasmids ID Plasmid Vector... Vector Type Promoter Selectable Marker PI Publication Return to top Multiplex gRNA Plasmids ID Plasmid...Plasmid Vector Type Promoter Selectable Marker PI Publication Return to top Multiplex gRNA Kits Many CRISPR...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...