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We narrowed to 243 results for: gRNA

Showing: 101 - 120 of 243 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...subsequently tested the original BE3 and HF-BE3 using a gRNA with high off-target cutting, and they found that... constructs (wild-type and truncation mutants), gRNAs used for TZAP gene editing, and a Hi6xs-MBP tagged...as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components can...compatible backbones that can be combined with your gRNA and repair templates of choice though modular cloning... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool...cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by anhydrous tetracycline....

  4. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...Then choose which Cas enzyme to use and design a gRNA to where you would like to introduce the edit. Finally....org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out our CRISPR Guide... (Yu et al., Nat Chem Biol). Mutate the PAM or sgRNA sequences Cas9 likely goes through multiple rounds...

  5. Design Tips for Prime Editing

    Type
    Blog Post
    ... a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer...extension of the pegRNA should not be C. A C base is speculated to base pair with G81 of the gRNA (Anzalone ... the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) can improve either...left), while pegRNAs that use other bases in this location bind to Cas9 with canonical sgRNA structure (...your tool of choice. Nicking sgRNAs PE3 and PE5: These tools use an sgRNA to guide nicking of the unedited... editors bound to pegRNAs drive editing (left), while prime editors bound to sgRNAs only drive nicking...Created with BioRender.com. RNA protectors epegRNAs: epegRNAs include structured motifs at their 3′ end...

  7. Plan Your Experiment

    Type
    Collection
    ...how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you have selected...enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence ...delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then... Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are combined...modify prior to designing your gRNA, as sequence variation between your gRNA targeting sequence and target...the associated gRNAs based on their predicted on-target and off-target activity ( see gRNA design software...containing validated gRNAs are now available through Addgene. These plasmids contain gRNAs that have been used...

  8. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to... questions to consider when choosing a gRNA: Does a given gRNA sequence exactly match your genomic target...Variation between a given gRNA sequence and your genomic target may reduce the gRNA activity. The variety ...pyogenes , S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...gRNA expression vectors available from Addgene. CRISPR...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...1143-8.  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively targets...DNA break. Next, the gRNA being tested is expressed along with Cas9. If the gRNA effectively cuts the ...for scientists to validate the efficacy of their gRNAs. The first step is to clone the target sequence ...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

  10. CRISPR Plasmids - Yeast

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...

  11. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Publication Empty gRNA Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...

  12. CRISPR Plasmids - Plants

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...pyogenes Bar Chen 53063 pU3-gRNA OsU3 AarI none S. pyogenes Gao 53061 pZmU3-gRNA maize U3 none S. pyogenes...pyogenes Gao 53062 pU6-gRNA wheat U6 BbsI none S. pyogenes Gao 59188 pBlu/gRNA Arabidopsis U6 BbsI none S. ...

  13. CRISPR References and Information

    Type
    Collection
    ...at Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 ...Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized ...optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo... cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and ssODNs design and...dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ....1016/j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...

  16. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...surrounding the DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the break ...single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can...

  17. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can...

  18. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...

  19. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...

  20. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...genome. gRNA Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector...Empty gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and a Cas ...pyogenes Puro Zhang phH1-gRNA 53186 Mammalian BbsI none S. pyogenes Gersbach pmU6-gRNA 53187 Mammalian BbsI...pyogenes Gersbach phU6-gRNA 53188 Mammalian BbsI none S. pyogenes Gersbach ph7SK-gRNA 53189 Mammalian BbsI...(U6a-BsaI-gRNA) 65955 Other/Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA) 65956 Other...
Showing: 101 - 120 of 243 results