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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 277 results for: mel

Showing: 241 - 277 of 277 results

  3. A Control for All Seasons

    Type
    Blog Post
    ...2009). If the fluorescence from your target is extremely bright, then the noise likely will not interfere...

  8. Molecular Biology Reference

    Type
    Guide
    ...may cause certain diseases. Sequencing DNA is extremely important when verifying plasmids to ensure each...only sequence short pieces of DNA. This is not extremely useful when trying to sequence an entire plasmid...

  9. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...2020. Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research. Curr...

  12. CRISPR Guide

    Type
    Guide
    ...A., Waldhauer, M. C., Börner, K., Fakhiri, J., Schmelas, C., Dietz, L., Grimm, D., Correia, B. E., Eils...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., ...

  15. Antibody Guide

    Type
    Guide
    ...kD single heavy domain fragments derived from a camelid antibody called a heavy chain antibody (HCAb). ...

  16. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... 2019 Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis...°C 30 sec / read plate/ repeat 39x from step 3 / melt curve Example of plate set-up: 1 2 3 4 5 6 7 8 A...accurately determine differences between samples. Melt curve analysis: a single peak should be seen. The...

  17. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step at 1-2 minutes... 3’ primers should be designed to have similar melting temperatures (Tm). Set the annealing temperature...

  18. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma #A9414 Luria Broth Agar (LB...37°C for 2 hours. Run digested DNA on 0.8% low melting point agarose gel until you can distinctly see ...

  19. Protocol - How to Design Primers

    Type
    Protocol
    ...60% G/C content Start and end with 1-2 G/C pairs Melting temperature (Tm) of 50-60°C Primer pairs should...

  20. Ligation Independent Cloning

    Type
    Protocol
    ...Use web-based primer design software to ensure a melting temperature between 50-60°C for your PCR primers...

  21. Fluorescence Titering Assay

    Type
    Protocol
    ... institution’s biosafety regulations. Workflow Timeline Day 0: Seed 293T cells Day 1: Transduce cells ...

  22. General Transfection

    Type
    Protocol
    ... and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T...

  23. Immunocytochemistry

    Type
    Protocol
    ...science. Last Update: January 20, 2022 Workflow Timeline Day 1: Seed cells Day 3-4: Fix and label cells...

  24. Lentivirus Production

    Type
    Protocol
    ... such as stable-cell line generation. Workflow Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect...

  25. Colony Formation Titering Assay

    Type
    Protocol
    ...cannot be used for later experiments. Workflow Timeline Day 0: Seed and transduce cells Day 2: Replace...

  32. Western Blot

    Type
    Protocol
    ...approaches. Last Update: January 24, 2022 Workflow Timeline Day 1: Prepare lysates, run SDS-PAGE, transfer...

  35. AAV Production in HEK293 Cells

    Type
    Protocol
    ...handle a large number of T-175 flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in ...

  36. CRISPR Library Amplification

    Type
    Protocol
    ...DNA pool. Last Update: August 17, 2023 Workflow Timeline Day 1: Transform, recover, set up overnight growth...

  37. Lentivirus ddPCR Titration

    Type
    Protocol
    ...regulations. Last Update: July 7, 2023 Workflow Timeline Day 1: Seed and transduce cells Day 4: Treat cells...
Showing: 241 - 277 of 277 results