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...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building CRISPR targeting...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Bacteria CRISPR Plasmids: Bacteria...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Edit Purify RNA Targeting Resources The following CRISPR plasmids have been designed for use in bacteria...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: ...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR C. elegans CRISPR Plasmids: C. ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that have been designed for use in C. elegans. CRISPR.... elegans Activate Cut Resources The following CRISPR plasmids have been designed for use in C. elegans...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... CRISPR Drosophila CRISPR...Activate Cut Nick Prime Edit Resources The following CRISPR plasmids have been designed for use in Drosophila...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Plants CRISPR Plasmids: ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR expression plasmids that have been designed for use in plants. CRISPR...Interfere Nick Prime Edit Resources The following CRISPR plasmids have been designed for use in plants. ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Xenopus CRISPR Plasmids: Xenopus Cut...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Cut Resources The following CRISPR plasmids have been designed for use in Xenopus. To find emtpy gRNA...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Yeast CRISPR Plasmids: Yeast Activate...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that have been designed for use in yeast. CRISPR...Cut Interfere Nick Purify Resources The following CRISPR plasmids have been designed for use in yeast. To...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Cut Resources The following CRISPR plasmids have been designed for use in zebrafish. To find emtpy...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...

...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community...plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library...published on the Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Parasites CRISPR Plasmids... Addgene's parasite CRISPR plasmid collection also includes the Toxoplasma CRISPR Knockout Pooled Library...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and...

...using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology for in ...vitro screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune...Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage...

...control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast ...podcast segment Anti-CRISPR (Acr) proteins potently inhibit type II CRISPR systems including the popular S. ... them a rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a ...domains, they have developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et al., ... protein AcrIIA4. In the dark, AcrIIA4 inhibits CRISPR activity.   With the addition of blue light, the...and thus, inhibiting function of Acr. This allows CRISPR-Cas9 to function on its target.  They name their... Acr-LOV fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity switching via a novel optogenetic...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...1: Overview of CRISPR base editing. Browse, sort, or search the tables below for CRISPR base editing plasmids...learn more about base editing and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR... CRISPR Base Edit CRISPR...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR RNA Editing CRISPR Plasmids...of RNA targeting with CRISPR. Browse, sort, or search the tables below for CRISPR plasmids designed for...learn more about RNA editing and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... Targeting CRISPR Plasmids: RNA Targeting Mammalian Bacteria Plant Resources Type VI CRISPR systems, including...for CRISPR plasmids designed for RNA targeting. To learn more about RNA targeting and other CRISPR topics...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...phage-infected bacterial cells so as to limit the spread of infection throughout the entire population. ...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... about visualization using CRISPR and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid... CRISPR Visualize CRISPR Plasmids:... using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for CRISPR plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...RNA Knockdown Visualize Resources The following CRISPR plasmids have been designed for use in mammalian...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...

... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Prime Edit CRISPR Plasmids: Prime Edit Mammalian Bacteria...below for CRISPR prime editing plamisds. To learn more about prime editing and other CRISPR topics, read...Ervin Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions...