...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...

...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link...plasmids! To get started with your deposit, please click here ....

...information on determining DNA concentration and purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid...

... of a heat resistant DNA polymerase from the thermophilic bacterium, Thermus aquaticus (Taq) . Taq polymerase...

... Marker PI Publication Plant Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...including that it is easy to implement due to the simplicity of gRNA design, programmable for different genomic...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you...

...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...efficiency than NHEJ. Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don’t See What You’re Looking For? Please...be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is available ...

...FPRL1, HM63, LXA4R FSHB follicle stimulating hormone, beta polypeptide - FSHR follicle stimulating hormone...into tables based on the Gene Lists from Immport . Click the links below to go to the gene table for each...HLAHP HSP90AA1 heat shock protein 90kDa alpha (cytosolic), class A member 1 FLJ31884, HSP86, HSP89A, HSP90A...LAP2 HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1 D6S182, FLJ26984, HSP90-BETA...component 5a receptor 1 C5A, C5AR, C5R1, CD88 CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39,...CALCRL calcitonin receptor-like CGRPR, CRLR CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39,...lambda receptor) CRF2/12, IFNLR, IFNLR1, IL-28R1, LICR2 IL29 interleukin 29 (interferon, lambda 1) IFNL1...

...Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, ...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...PMID: 28814507 Allen Institute for Cell Science Publications...

...synthetic operons for chloroplast transformation and metabolic engineering in plants (including tobacco and potato...community. Grassius (Link opens in a new window) - a public web resource composed by a collection of databases...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

...known to function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene...help of . Return to top p53 Pathway - Gene List Click on a name to find available plasmids for the gene...PubMed PMID: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective...

...quantification. This protocol was modified from the publication Lock et al. (2014) (Link opens in a new window...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... supplemental materials from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively...Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, ...

... layers are covered with media. 293T cells are delicate and detach very easily - media should always be...

...advantage of using AAV for research is that it is replication-limited and typically not known to cause disease...generally contained at lower biosafety levels and elicit relatively low immunological effects in vivo . ...certain exogenous gene products that mediate AAV replication. This requirement has been circumvented with ...