... been modified for genome engineering . Prior to CRISPR, genome engineering approaches like zinc finger...rapidly become the most popular genome engineering approach. Engineered CRISPR systems contain two components...Cas-mediated genome engineering. 2013. Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch...Activation or Repression Epigenetics Multiplex Genome Engineering Genome-wide Screening What are pooled libraries...mutation within the targeted gene. However, the strength of the knockout phenotype for a given mutant cell...generation of high fidelity enzymes have been engineered using cell-based selection approaches. Yeast-...target (termed left & right homology arms .) The length of each homology arm is dependent on the size of...

...specific genes Oligomerized Pool ENgineering (OPEN) Reagents - Engineer zinc finger arrays via OPEN method...method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular Assembly method ...nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium...promote continued research and development of engineered zinc finger technology. Consortium members Keith...have deposited at Addgene various reagents for engineering and expressing zinc finger proteins for distribution...reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes...

...conversion tract length in drosophila. This 1998 study suggests mammalian cells' tract lengths seem much shorter...first author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a list of FAQs about ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR...CRISPR plasmids General CRISPR Plasmids Genome Engineering Videos from Le Cong Dr. Le Cong, the first author...author of one of the seminal articles from Dr. Feng Zhang's Lab ( Science , February 2013 ), has assembled...wildtype or double nickase for my CRISPR genome engineering experiments? When assessing which nickase type...type to use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized ...

...crRNA(SOD1) SOD1 hSyn1 ALS Feng Zhang 109322 pU6-crRNA(TBK1) TBK1 hSyn1 ALS Feng Zhang 109323 pU6-crRNA(TARDBP... FXN EF1a Friedreich ataxia Feng Zhang 142079 TFORF2441 FUS E1Fa ALS Feng Zhang 142463 TFORF0142 FXN E1Fa...E1Fa Friedreich ataxia Feng Zhang 142874 TFORF1856 NR4A2 E1Fa Parkinson's Feng Zhang 142875 TFORF1857 ...TFORF1857 NR4A2 E1Fa Parkinson's Feng Zhang 142944 TFORF2440 FUS E1Fa ALS Feng Zhang 143027 TFORF0449 DNMT1 E1Fa...sensory neuropathy type IE Feng Zhang 143542 TFORF1771 TBP E1Fa Parkinson's Feng Zhang 143543 TFORF1772 TBP...Parkinson's Feng Zhang 143702 TFORF0450 DNMT1 E1Fa Hereditary sensory neuropathy type IE Feng Zhang 144906...LRRK2-K1906M LRRK2 His, Myc CMV Parkinson's Zhaoyang Feng 36004 Park2_L (TAL 3012) PRKN Flag, FOKI CMV Parkinson's...

...Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish...plasmids and strains used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method. This kit consists...). The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish...of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

...Cre-driven recombination. CREM (or Cre-M) has been engineered to contain an intron, preventing Cre expression...Cre-EBFP fusion; Expression in neurons. synapsin AAV Zeng 51904 paavCAG-iCre iCre CAG AAV Kim 55632 pAAV-Ef1a-mCherry-IRES-Cre... Cre; Targeting vector for Pvalb locus Mammalian Zeng 61573 Rasgrf2-2A-dCre targeting vector DHFR-destabilized...DHFR-destabilized Cre; targeting vector for Rasgrf2 Mammalian Zeng 61574 Slc17a7-IRES2-Cre targeting vector Cre; Targeting...Targeting vector for Slc17a7 locus Mammalian Zeng 62219 Nxph4-2A-CreERT2 Targeting Vector Cre-ERT2 - Tamoxifen...inducible; Targeting vector Mouse Nxph4 Mammalian Zeng 62372 pET-(-30)GFP-9xGGS-Cre-6xHis Cre fused to ...2A-mCherry-FKF NLS-Cre, mCherry T7 Bacterial Sauka-Spengler 119971 GAG-CRErec GAG-Cre fusion; for the production...

...Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide...Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL...Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents Assembly via serial ligation. Validated... cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly Kit Assembly by ...cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors...domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena...three 0.5 TALE repeats (C, T, and G) and the full length human LSD1 at the carboxy-terminus of the protein...

...pAAV-EF1a-iCreV EF1a none 1, PHPeB Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Zeng 140137 pAAV-EF1a-iFlpV EF1a...PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s... PI 51669 AAV phSyn1(S)-FlpO-bGHpA hSyn none rg* Zeng 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (...121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 8, 9, rg* Engel , Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none ...Synapsin Promoter 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP...EF1a none 1, PHPeB Zeng *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from ...

...Arrays Zinc Finger Consortium: Zinc Finger Arrays Engineered Zinc Finger Arrays for Endogenous Zebrafish Gene...Targets Recent published reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools... Joung, Randall Peterson, and Joanna Yeh are engineering zinc finger arrays targeted to various zebrafish...Addgene--the specific vector to use depends on the length of the spacer sequence between the half-sites (...zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full Target Site (5' to 3') Left half-... publication. References Rapid 'open-source' engineering of customized zinc-finger nucleases for highly...301. PubMed PMID 18657511 . Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

...SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson...Weiss Synthetic biology describes the rational engineering of biological systems for novel functions. Addgene...sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain functional...Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular...Community Resources iGEM - International Genetically Engineered Machine Foundation Registry of Standard Biological...Bio-Energy Institute Synberc - Synthetic Biology Engineering Research Center The PLOS Synthetic Biology Collection...

... molecular biology technologies: from genome engineering and gene regulation to fluorescent proteins. ...modification of monocot and dicot genomes using genome engineering; validated in tomato, tobacco, M. truncatula ...for wheat and other monocots - Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for non-transgenic...genome editing across crop species and genotypes - Zhenghe Li. A robust CRISPR/Cas9 system for convenient,...silencing rescue in plants - Martin Parniske. GreenGate Cloning System to create plant expression vectors...for chloroplast transformation and metabolic engineering in plants (including tobacco and potato) - Scott...

...research, and to find plasmids that can be used to engineer viruses for a wide array of research purposes....these links to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids ...to generate virus. A) Viral plasmids have been engineered such that they can be delivered to a host and...through standard plasmid cloning, viruses can be engineered to harbor a wide array of viral genomes, enabling...better able to remember how to solve a maze. These engineered viral genomes are also modified (relative to ...

... Genome Engineering CRISPR Fujii Lab CRISPR Plasmids Fujii Lab CRISPR...insertional chromatin immunoprecipitation (iChIP) and engineered DNA-binding molecule-mediated chromatin immunoprecipitation...enChIP, specific genomic regions are tagged with engineered DNA-binding molecules such as TAL proteins and... and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...genomic regions. Hoshino A, Fujii H. J. Biosci. Bioeng. 2009 Nov;108(5):446-9. doi: 10.1016/j.jbiosc.2009.05.005...

...recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana Gradinaru's ...distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana Gradinaru...interest, and model organism under study. *Many engineered AAVs for mice use LY6A to cross the blood-brain...pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3...

...Genome Engineering: Basic Principles Genome Engineering: Existing Technologies Get Genome Engineering tools... Genome Engineering Videos CRISPR Technology and Applications CRISPR-Cas...performed by Patrick Hsu, David Scott, and others in the Feng Zhang Lab. The CRISPR-based transcriptional modulation...

...1504. Xiaodong Liu Calcium NEMO calcium sensors Engineering of NEMO as calcium indicators with large dynamics...indicator for two-photon imaging at fiber laser wavelengths. Nat Methods. 2020 May 25. pii: 10.1038/s41592...dynamics of ATP in single cells. Angew Chem Int Ed Engl. 2018 Jun 27. Tetsuya Kitaguchi ATP Variants of ...neurons, astrocytes) A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface...insect expression (Cre-dependent) cAMPr: A single-wavelength fluorescent sensor for cyclic AMP. Sci Signal...measurement Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science...Nanosensors for in vivo measurements of citrate (CitA) Engineering genetically encoded nanosensors for real-time...

...pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-9-ALL864 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-5-PV2509 29777...pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-9-ALL856 100048-AAV9 pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-8-PV0142 105534-...ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-1-PV0102 105531-AAV1 pAAV.CMV.LacZ.bGH James ...ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH...ALL854 51502-AAV9 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-9-PV0101 105530-AAV9 pAAV.CMV.PI.EGFP.WPRE.bGH...

... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR...Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation... References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox.... 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD,...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD...Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440...

...emit a specific wavelength of light after excitation by light of a specific wavelength. While more expensive...monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin repeat proteins... constant region. Minibodies - Minibodies are engineered antibody fragments consisting of two scFvs bound... in a range of colors activated by different wavelengths, allowing for easy differentiation between multiple...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ... break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may need to be optimized...

...Background Chemogenetics plasmids encode genetically engineered receptors that are activated by specific small...control signaling within cells expressing these engineered receptors. Chemogenetic receptors are popular...behavior. Depending on the signalling pathway the engineered receptor is coupled to and the specific cell ...are G-coupled protein receptors that have been engineered to respond to specific physiologically inert ...while hMD4(Gi) and KORD are inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains...