... mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP AICSDP-24 mEGFP Non-muscle ...AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43...69 mTagRFP-T Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar...

... of p53’s pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...Phosphatase and tensin homolog PUMA BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate...

...University changed the way researchers can explore important questions surrounding serine phosphorylation by...in place of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP...188531 ; 188532 ; 188533 ; 188534 ; 188535 ). Importantly, phosphorylation positional isomers are allocated...

...biomolecule while largely preserving spatial and temporal cellular processes. Note that many of the articles...RGB-color intensiometric indicators visualize spatiotemporal dynamics of ATP in single cells. Angew Chem...or Cre-dependent) A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics...HT3.0 Dual-color GRAB sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566...lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1...

...RNA-guided transcriptional activator system with two components, the dCas9 activator protein and the single guide...provides the transactivation activity, the sgRNA component provides the specificity and is designed to target...

...identify novel interactions or pathways that are important in cellular recognition processes. Laboratories...protein interaction network and its resolution by spatiotemporal expression profiling. Martin S, Söllner C, ...

...pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi none S. pyogenes Blast Docampo pDonor_hU6... and plasmids that provide Cas9 variants. All components are then assembled into a single T-DNA binary...

...collection differ based on some important criteria, and it is important to know which library best suits...suits your particular experiment. Some important variables include: Type of genetic modification - CRISPR...

... Different types of viruses also vary in the composition of their viral genome, with some having an RNA...for long term studies) while other viruses are temporary in the host. Use this guide to learn more about...

... to find available plasmids for the gene. For components with multiple subunits or isoforms, individual...EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast growth...

...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...

... cluster in the MBD and NID demonstrating the importance of binding to methylated DNA and the recruitment...syndrome at the molecular level. They are also an important tool for developing therapeutic strategies to ...

...sub-populations has generally been limited to single-component cellular definitions (e.g. neurons defined by ...working with INTRSECT recombinases, viruses, and important controls to consider as part of experimental design...

...libraries can be used to identify genes that are important to a given phenotype. Current libraries are available...results! Getting Started with CRISPR Addgene has empowered researchers to further develop the CRISPR toolkit...

...numbers is acceptable. The bulk sorted cells are composed of a polyclonal population exposed to sgRNA-A ...6.3 . Assemble a 20 μl PCR with the following components: 10 μl 2x PCR mix, 0.5 μl forward primer (10 ...

...biology plasmids related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways...

...scientists can design de novo systems using modular components or take advantage of existing GFP-lines for cell-specific...

...protein interaction network and its resolution by spatiotemporal expression profiling. Martin S et al., Mol ...

... targeted genome editing. RGNs consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses...

... protein as a fusion protein in which the two components are joined by a T2A self-cleaving peptide sequence...