...E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related... SHC4 SHC (Src homology 2 domain containing) transforming protein SHOC2 SHOC2 leucine-rich repeat scaffold...

...Gate-assembled TALEN, both in microinjected embryos and transfected cells. TALEN expression is driven by the strong...expression in mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI)...on pTAL7a and pTAL7b for enrichment of double-transfected mammalian cells. In addition, a T7 promoter was...alternative. Based on transient double-selection of transfected cells, the system has successfully been employed...

...includes listing of transfer status for viral preps.... Viral Service Penn Vector Core Transfer Viral Service:...Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn Vector Core) and Addgene...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...

... dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu U6-BbsI-crRNA dU6 BbsI Transfection none, need ...Harrison, Wildonger pU6-BbsI-chiRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles, Harrison...

... Pennsylvania Vector Core . Transfections are performed using the transfer plasmid, a plasmid encoding... taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching...

...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture cells... plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target...following plasmids can be used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma...plasmids can be used to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma... recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells....

...human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols... #61422 - #61424 were designed for transient transfection : 61422 : Expresses dCAS9-VP64 activator with...codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to ...

...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...

...fluorescent protein (cpFP) or Förster resonance energy transfer (FRET) efficiency of a pair of fluorescent proteins...in plant cells Photosynthesis-dependent H 2 O 2 transfer from chloroplasts to nuclei provides a high-light...metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of pentose...temperature indicator B-gTEMP Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature...

...different cell types. Zinc finger arrays can be transferred in-frame to these vectors....either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives...

... different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning grade DNA...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...

...none, need Cas9 plasmid Joung pMB70 cU6 BsaI Transfection none, need Cas9 plasmid Boxem pMB60 T7 BsaI ... Boxem PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid Calarco SP6-sgRNA-scaffold...

...dCas9 activators and sgRNAs can be separately transfected (pmax dCas9 activators [48223~48227] + sgRNA ...Gateway donor vectors [48218~48221] and can be transferred to Gateway destination vectors (e.g., pmax-DEST...

...both plasmids. This DNA molecule can then be transfected into mammalian packaging cell lines to generate...of interest? Search our database for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description...

...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...Single cell passaging of human iPS cells RNP transfection for gene editing human iPS cells Reference Roberts...

...interfering RNA) via transfection, or shRNA (short hairpin RNA) via transfection or viral delivery of ...

...retroviruses, are the most popular virus for gene transfer in clinical trials. These viruses have a relatively...the delivery of genetic information to hard-to-transfect mammalian cells. In addition, "viral pseudotyping...

...Addgene AAV transfer plasmids...viral genome (typically called cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description...

...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies.... an SDS-PAGE gel to separate proteins by size. Transfer proteins from the gel to a membrane. Incubate ...