...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy in living cells....

...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Plasmids MORF Pooled Library The MORF library is a comprehensive human transcription factor collection that contains...Regulatory Factors (MORF) pooled library is a comprehensive collection of human transcription factor open...

... research in small biotech and pharmaceutical companies. The RESOLUTE project has received funding from...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow...efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry...

...FLAG prey CD200-Cd4d3+4-COMP-blac-3xFLAG-6his Cd4d3+4-pentamer BasiginCd4d3+4-COMP-his-stop Type II expression... Type Plasmid Name Bait Cd4d3+4-bio Prey Cd4d3+4-COMP-b-lac His-tag Cd4d3+4-6his Bio-linker-his Cd4d3-...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...

...Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement (MTA), including...Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using...using the materials in the order. After the RSA is complete, Addgene will send you a copy of the MTA, which...

...below and not included in this table. For a more complete list of plasmids that can be used in worms, search...elegans. WormBase - A consortium of biologists and computer scientists dedicated to providing the research...elegans and related nematodes. WormBook - A comprehensive, open-access collection of original, peer-reviewed...

...juxtaposed to the DNA complementary region. The uniqueness of CRISPRi, as compared to several recently ...uide RNA (sgRNA) designed with a 20-basepair complementary region to any gene of interest. Co-expression...

... - a public web resource composed by a collection of databases, computational and experimental resources...monocots and dicots - Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency...

... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...Phosphatase and tensin homolog PUMA BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate..., Lu X, Soron G, Cooper B, Brayton C, Park SH, Thompson T, Karsenty G, Bradley A, Donehower LA. Nature...

...intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F) serotype...in the retina following subretinal injection as compared to the parental AAV44.9 serotype. It was developed...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...

...for use in CRISPR experiments. This datatable is compiled from plasmids in Addgene's database as well as...designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant ...Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting...the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable Target Gene...

...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA...endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to clone the... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...

...system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design but...Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer...how closely homologous the off-target sites are compared to the on-target site, the specific site sequence...

...designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant ...Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting...

...knockout, repress, and activate gene targets. To accomplish this they have taken advantage of the Zhang ...guide within a U6-guide cassette. This process is compatible with all pX458 family plasmids. Additional Cloning...

...RNA-guided transcriptional activator system with two components, the dCas9 activator protein and the single guide...provides the transactivation activity, the sgRNA component provides the specificity and is designed to target...

...γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic...gets integrated into the host's genome by the accompanying integrase protein. When scientists discuss retrovirus...

...recombinant antibodies, created by cloning antibody components into plasmids and expressing these in bacteria... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...

...different experimental goals. How does this method compare to other similar methods? Where does this method...For All Attendees: To help us best prepare and compile resources for this meeting's publication, we are...