Skip to main content
Addgene
Showing: 1 - 30 of 45 results

  1. Genetic Code Expansion

    Type
    Collection
    ...redirecting specific codons to encode amino acids beyond the 20 standard amino acids. Expanding the Genetic Code...translation, an aminoacyl-tRNA synthetase (aaRS) loads its cognate tRNA with a specific amino acid. Then, ...cognate aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids ...incorporate a non-canonical amino acid into the protein of interest: The non-canonical amino acid, which is generally... recognizes this codon. An aminoacyl-tRNA synthetase to load the new amino acid onto the tRNA. The tRNA...pANAP AnapRS E. coli 3-(6-acetylnaphthalen-2-ylamino)-2-amino-propanoic acid (Anap) Mammalian TAG Peter ...structurally analogous amino acids Bacterial TAG Ryan Mehl 85502 pDule-para-aminoPhe pAF synthetase M. jannaschii...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...CMV Spinocerebellar ataxia 15 Ryohei Yasuda 112270 CMV-PKCg-mEGFP-N1 PRKCG GFP CMV Spinocerebellar ataxia...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...

  3. Immunology Research Plasmids and Resources

    Type
    Collection
    ...ULBP4, bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...PF4A, SCYB4V1 PLAU plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase...KGFLP1 keratinocyte growth factor-like protein 1 MGC125746, MGC125747, MGC126891 KGFLP2 keratinocyte growth... DG6, PMBP PLAU plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase...protein 2 FLJ23282, FRA RARA retinoic acid receptor, alpha NR1B1, RAR RARB retinoic acid receptor, beta HAP...NR2B1 RXRB retinoid X receptor, beta DAUDI6, H-2RIIBP, MGC1831, NR2B2, RCoR-1 RXRG retinoid X receptor...

  4. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus via a 17 amino acid linker C32V Cerulean...attached to Venus via a 32 amino acid linker C5A Cerulean attached to Amber via a 5 amino acid linker C17A Cerulean... to Amber via a 17 amino acid linker C32A Cerulean attached to Amber via a 32 amino acid linker mGFP-10...Monomeric EGFP attached to super-REACh via a 10 amino acid linker ACA Heterotrimeric construct consisting...two Venus fluorescent proteins connected via a 5 amino acid linker V17V Anisotropy/brightness standard ...two Venus fluorescent proteins connected via a 17 amino acid linker V32V Anisotropy/brightness standard ...two Venus fluorescent proteins connected via a 32 amino acid linker VVV Anisotropy/brightness standard consisting...

  5. CRISPR Guide

    Type
    Collection
    ...base editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...to small indels in the target DNA that result in amino acid deletions, insertions, or frameshift mutations...enzymes. hfCas9s were developed by mutating specific amino acid residues to reduce off-target editing. Some... prime editing, and are often used to change an amino acid, turn a promoter on/off, or add small protein...with a silent mutation that does not change the amino acid sequence. HDR efficiency is generally low (...editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating...soon followed, using an enzyme evolved to excise inosine to access the same repair pathway and convert A...

  6. Bacterial Expression Systems

    Type
    Collection
    ...Wolf Frommer Reporter for arabinose levels in bacteria. The presence of arabinose enhances the FRET signal...14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression of an N-terminally His-tagged...14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression of an N-terminally His-tagged... LIC Series (8-Series) Various Plasmids pBAD (arabinose inducible, glucose repressible) His, Strep, mOCR...TEV cleavage sites to remove tags Scott Gradia Arabinose inducible vectors that come with various epitope...Various Plasmids pBAD, OR2-OR1-PR, pLtetO, pLlacO Arabinose, Anhydrotetracycline, lactose, IPTG Richard Murray...from the CFP and YFP fluorophores linked by an arabinose binding protein (AraF) in the plasmid. Other similar...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine is translated as guanosine, so the overall...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR genes employ varied mechanisms ...MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA...Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F. 2017. Multiplex gene...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...1147-1155. Lin Tian Endocannabinoid GRAB_eCB2.0/eCBmut biosensor for endocannabinoids in vivo (Constitutive...CEPIA. Nat Commun. 2014 Jun 13;5:4153. Masamitsu Iino Calcium Red mitochondrial CEPIA calcium sensors ...mitochondria. Sci Rep. 2020 Feb 18;10(1):2835. Masamitsu Iino Calcium Red fluorescent calcium sensors (fRCaMP1...with an Expanded Dynamic Range by Optimization of Amino Acid Linkers between a Fluorescent Protein and PDE5alpha...sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo. Nat Biotechnol. 2021 Nov ... J Neurosci. 2006 Feb 15. 26(7):2000-9. Robert Malinow Endo/Exocytosis Synaptophysin-pHluorin Clathrin-mediated...

  9. NETRF

    Type
    Collection
    ...discover cures and more effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying...Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ... Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell ....top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ...mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids as well as a backbone...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  11. Ras Pathway

    Type
    Collection
    ...Platelet derived growth factor receptor PDPK1 3-phosphoinositide dependent protein kinase 1 PEBP1 Phosphatidylethanolamine...PIK3R4 PIK3R5 PIK3R6 Also known as PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and ...phosphatase 1 catalytic subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange...RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 ...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Library 169942 Knockout Human Ebert 3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR...CRISPR KO library 174592 Knockout Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries...Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 ...Knockout Human Doench and Root 3rd 4 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout...

  14. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...RNA editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine... gRNA. Adenine base editors convert adenine to inosine, which is replaced by guanosine to create A->G ...

  15. TALEN Expression Vectors

    Type
    Collection
    ...TAL effector repeat arrays with identical DNA and amino acid sequences (i.e. only the details of the assembly...epitope tag and nuclear localization signal at their amino-terminus. The expression vectors listed below can...

  16. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...RNA editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...

  17. Brain Initiative Collection

    Type
    Collection
    ...for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable...for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable...

  18. mTOR Pathway

    Type
    Collection
    ...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy levels, and growth...PIK3R1 PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and ...

  19. Microbiology Resources

    Type
    Collection
    ...Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. ...assembly of up to three transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway...

  20. TALEN Guide

    Type
    Collection
    ...composed of 33-35 amino acid repeats. These repeats only differ from each other by two amino acids, their ...

  21. Zebrafish Plasmid Collection

    Type
    Collection
    ...transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9...Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag...

  23. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...serine phosphorylation by adding this phosphorylated amino acid to the genetic code of E. coli (Park et al....consist of a central phosphoserine site flanked by 15 amino acids on either side occurring within the parent...

  24. TALEN Plasmids and Kits

    Type
    Collection
    ...determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. View Addgene'...DNA-binding ability of TALEs), 63 wild-type TAL amino acids after the repeat domain, and C-terminal SV40...

  25. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...incubate O/N at 37 °C. Pick 2 - 3 colonies and inoculate into a mini-prep culture. Perform mini-prep for...utilized. Choose a sequence-verified colony and inoculate into a maxi-prep culture. Prep size may be scaled...

  27. KLF Research Plasmids

    Type
    Collection
    ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...

  29. Chemogenetics Plasmids

    Type
    Collection
    ...Another recently developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins...

  30. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which is present in many...
Showing: 1 - 30 of 45 results