... Distribution to Industry Featured Collections Search Plasmids...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from...Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf of the depositing institutions...the language which we are allowed to use for distribution. Please see the Sample Agreements on our Technology...order will be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is ...

... Anti-Beta4-spectrin [N393/2R] Beta4-spectrin Human Mouse IgG2a 177542 Anti-Beta4-spectrin [N393/76R] ...Species Host Species Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4...SUR1 Rat Mouse IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558...SynCAM4 Mouse Mouse IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a 128612...IgG2a 149455 Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R] MMP9 matrix metalloproteinase-9 precursor...Ca2+ channel Rat Mouse IgG2a 164154 Anti-TRIP8b [N212/7R] TRIP8b Rat Mouse IgG2a 164155 Anti-GABA(A)R, alpha6...LRP4 Mouse Mouse IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515...

... Concentration 100 ng/µl (± 5ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and...

... be for efficient recombination? The most we've tried to insert so far has been 1kb. We used homology ...template after performing gDNA purification and have tried conditions with 5% DMSO or without 5% DMSO. My genomic...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ( see Figure...

... sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for in vitro as well as in vivo...PMID: 24191859 Ye, et al. 2015. Safety and Biodistribution Evaluation in Cynomolgus Macaques of rAAV2tYF-CB-hRS1...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Mol Ther Nucleic Acids . Sep...

...Run samples on 2% agarose gel at 10 V/cm using 1x Tris-acetate-EDTA (TAE) buffer. Examine samples for the...step 3.1.4 ), 5.0 μl restriction enzyme buffer (10x), 4.0 μl (20 U) BbsI restriction enzyme (5,000 U/ml)...for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after ...-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for 5 min and remove supernatant...

... Plant TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine synthetase M. jannaschii... Mehl 163915 pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial...Huber 177311 pRSF-G1TFAKRS M. archaeon N6-(trifluoroacetyl)-L-lysine (TFA-Lys) Bacterial TAG Thomas Huber...200226 pMega-MaFRSA PylRS M. alvus meta-trifluoromethylphenylalanine Bacterial TAG Alanna Schepartz 201922...

...AiE0621h SYFP2 Olfactory tubercle and Fundus of striatum Striatum 214849 pAAV-AiE0621h-minBG-iCre(R297T)-BGHpA...AiE0621h Cre Olfactory tubercle and Fundus of striatum Striatum 191717 pAAV-3xSP10ins-AiE0367h_C2-minRho-SYFP2...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum Whole brain Addgene...AiP13038 AiE0743m_3xC2 SYFP2 Cholinergic interneurons Striatum 214848 pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA...AiP13780 AiE0743m_3xC2 Cre Cholinergic interneurons Striatum 214858 pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG...AiP14496 AiE0873m_3xC2 SYFP2 Cholinergic interneurons Striatum 214868 pAAV-AiE0873m_3xC2-minBG-iCre(R297T)-BGHpA...

...biosensor for cyclic-di-GMP measurement Asymmetrical distribution of the second messenger c-di-GMP upon ...redox-sensitive ratiometric sensor roGFP for mitochondria or cytosol Hypoxia triggers subcellular compartmental...depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of...imaging (GECOs), including blue, green, red, and ratiometric indicators An Expanded Palette of Genetically...10.1117/1.NPh.11.2.024207. Robert Campbell Calcium Ratiometric calcium biosensors from nested reporters of green... green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green...anisotropy Cameleon calcium sensors Single-color, ratiometric biosensors for detecting signaling activities...

...BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru...BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru...protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru...BGHpA (Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs...BGHpA (Alias: CN4360) Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) ...BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs...BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) ...

...KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 TNFRSF10C tumor necrosis factor... to a specific trigger. Symbol Name Synonyms ACVR1B activin A receptor, type IB ACTRIB, ACVRLK4, ALK4,... A receptor, type IIA ACTRII, ACVR2 ACVR2B activin A receptor, type IIB ACTRIIB, ActR-IIB, MGC116908 ACVRL1...secretagogue receptor - GIP gastric inhibitory polypeptide - GIPR gastric inhibitory polypeptide receptor...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...

...CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina Schroer...H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch SOD1 H1 ALS Patrick Aebischer...CMV Parkinson's Patrick Aebischer 36070 pAAV asyn S87A/S129A SNCA CMV Parkinson's Patrick Aebischer 36071...EGFP OPTN GFP CMV ALS Beatrice Yue 68839 pOPTN1-209-EGFP OPTN GFP CMV ALS Beatrice Yue 68840 pOPTN1-424...EGFP OPTN GFP CMV ALS Beatrice Yue 68841 pOPTN217-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68842 pOPTN217-...EGFP OPTN GFP CMV ALS Beatrice Yue 68843 pOPTN425-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68846 pR96L-EGFP...pR96L-EGFP OPTN GFP CMV ALS Beatrice Yue 68847 pD474N-EGFP OPTN GFP CMV ALS Beatrice Yue 68848 pE478G-EGFP OPTN...

...Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1 - Mammalian...Expression mAmetrine 406 526 26 6 0.8 hr Monomer (A206K) pBad-mAmetrine - Bacterial Expression mAmetrine-N1 -...- Mammalian Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C)... Expression pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus...Mammalian Expression mCitrine-C1 - Mammalian Expression mCitrine-pBAD - Bacterial Expression YPet 517 530 80 ... - Mammalian Expression mAmetrine-C1 - Mammalian Expression LSSmOrange 437 572 23 5.7 2.3 hr Monomer pLSSmOrange-C1...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many...

...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth...-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth ...-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth ...pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Roth 50476 ...pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine none 5 Roth 50478 pAAV-GFAP-hM3D...element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent...

...-CDS issues. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI Restriction Sites to Avoid: NotI For...Coli genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI, PstI Restriction Sites to Avoid: PvuII, ... use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix...assembly standards most commonly used by part registries, including the specific prefix and suffix sequences... reverse complimented for primer design. The restriction enzymes used for assembly are the prohibited ... Employs high-efficiency enzymes. Prohibited Restriction Sites: EcoRI, BglII, BamHI, XhoI For more info...giving in-frame scar encoding Thr-Ala. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI For more info,...

...easily visualize products from an AgeI/EcoRI restriction digest) David Root 10879 pLKO.1 ‐ TRC control...shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono 11779 pLVCT-rtTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier Trono 11644 pLVET-tTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono 11642 pLVPT-tTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono 11652 pLVPT-rtTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier Trono 11651 pLVUT-tTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono 11648 pLVPRT-tTR-KRAB...

...opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in a new...in a new window) PMID: 17289941 2010 - Allyson Muotri's lab develops the first iPSC model for Rett syndrome...: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link opens...: 26944080 (Link opens in a new window) Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts & iPSC...new window) HRV Metrics Toolbox - algorithms for data analysis to calculate heart rate variability (Link...in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in a new window)...Return to top Help us improve this resource by contributing your plasmids! To get started with your deposit...

...Dyche Mullins 41147 pEGFP Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 ...KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome transport signal dL5 FAP; mCerulean3...41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres... mCherry Gia Voeltz 89472 GFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 GFP Ken-Ichi Takemaru ...Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1...EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru 54234...-alpha Tubulin Microtubules alpha-tubulin EGFP Patricia Wadsworth 27123 eTC GFP beta-actin full length...

...undergo stringent quality control by Addgene or a trusted quality control partner before distribution. Details...Production Antibody Production Many antibodies distributed by Addgene have been produced in-house by Addgene...contacting [email protected] . Some of the antibodies distributed by Addgene were developed by the Institute for..., antibodies undergo a buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered.... Only antibody samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes...

...fingers that recognize various nucleotide triplets. With some trial and error, ZF arrays are able to recognize...zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer, cutting...their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and interactions... targets are confined to sequences composed of triplets with corresponding zinc fingers, potential targetable...genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start with a T...

...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...single antibody signal. Here, HRP is used as a colorimetric, in which the reaction produces a colored product...the amount of enzyme present in each well. In colorimetric assays, protein amount can be determined by ..., lyse, and then collect protein extract by centrifugation or affinity columns. Mix samples with a primary...immobilized on agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic... your samples using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific...tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule may be a...

...Addgene distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as ...NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection . The NeuroMab Facility and James Trimmer have produced...Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from...that are currently in our repository. AAV are distributed as 100 µL of purified viral particles in solution...

...subjected to quality control by Addgene before being distributed to customers. Details about our production protocols...control are described below. AAV Production AAV distributed by Addgene has been produced either in-house ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone...

...secreted version of the protein is available – these attributes have made it popular with scientists since its...enchancer regions in Drosopholia Herman Wijnen 126033 pTETRIS-cargo Firefly For use as a reporter of the ability... in plants, co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 pRATIO1267 RedLuc...plants, co-expressed with Gaussia luciferase for ratiometric imaging David Nelson 212935 pGL4.84(hRlucCP/Puro...Lentiviral expression of firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral...

...101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-...lines have been deposited here with Addgene for distribution to the research community. With these plasmids...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...

...amplification Viral vector production Density centrifugation purification Addgene’s comprehensive quality... and results through Addgene’s Data Hub . By contributing your AAV vector experience, from methods to ...titration, and quality control measures. We have distributed preps around the globe and shared our well-developed...lab, and even managing legal permissions and distribution. Troubleshooting If your answer isn’t in the...

...candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin...stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated through both phosphorylation...ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E ...

... super-REACh via a 10 amino acid linker ACA Heterotrimeric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-6aa linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Venus...linker-Venus VCA Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Amber VCV ...VCV Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric...

...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

...a promoterless vector with several available restriction sites none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre...Muscle-specific expression of Cre MCK Mammalian Kahn 13763 pTriEx-HTNC Cre fused to HIV-TAT - promotes cellular uptake...puro Luciferase - EGFP- Cre fusion CAG Mammalian Stringer 68448 TOPO rtTA3-2A-Cre iRFP670, Cre, and GFP ...Luo 119063 pBac(3xP3-gTc’v; Tc-hsp68:Cre) Cre Tribolium casteneum hsp68 T. castaneum (red flour beetle...Ohlmann 120279 014LP-pAAV-ANF-657- Cre-pA Cre; for atrial-specific expression ANF AAV Wehrens 121675 pAAV-EF1a-fDIO-Cre... partnership with genOway, Addgene is able to distribute materials containing FLEx technology. Read the...

... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ...agarose gel to identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For small nucleotide...nucleotide edits that introduce a novel restriction site. The region of interest is PCR amplified, digested...digested with the appropriate restriction enzyme and run on an agarose gel to identify DNA fragments. PCR...

...protein resources here . pCyPet-His 14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible ...YPet) found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible ... and P. pastoris . All plasmids use the same restriction enzymes for cloning so multiple constructs can...compatible with standard cloning using many different restriction enzymes. pPro24-gfp contains GFP but all others...

...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... Acidaminococcus sp. ). The popularity can be attributed to Cas12a’s many benefits. Cas12a exhibits increased... quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2 to...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... (12), 770–788. PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....152 (5), 1173–1183. PMID: 23452860 Savic, D., Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows...

...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander...encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These...

...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S...Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion.... 2017 Aug;9(8):1117-1131. Parmar Fibroblasts Dentritic Cells Lentiviral Mouse Direct reprogramming of...

... viral vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality...genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified viral particles...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde...

...as in-stock preps. These high-titer preps are distributed as 100 µL solutions of purified viral particles...Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core... have the right to use the original plasmid. Distribution of the original plasmid is important because...

...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...Addgene continues to monitor its library and distributions in accordance with export laws and biosafety...Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. ...

... Other/Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA) 65956 Other/Tribolium castaneum...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations... pKS diaCas9_sgRNA 74923 Other/Phaeodactylum tricornutum U6 yes, cut S. pyogenes Winge pJJR50 75026 Worm...

...Addgene has partnered with Ginkgo Bioworks to distribute SARS-CoV-2 plasmids. The collection contains:... a for-profit entity, please see our Plasmid Distribution to Industry page. The list of plasmids available...Cathepsin L - a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating...