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We narrowed to 39 results for: mme

Showing: 1 - 20 of 39 results

  1. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...The NeuroMab Facility and James Trimmer have produced monoclonal antibodies validated for neuroscience...Collection NeuroMab Plasmid Collection NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids...the laboratory of NeuroMab Director, Dr. James Trimmer, have produced numerous monoclonal antibodies (...the culture media. For high level expression we recommend using the HEK-2936E cell line engineered by Durocher...e9. PMID: 11788735 (Link opens in a new window) Trimmer J, et al. 2020. Recombinant Antibodies in Basic...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...levels and transport (FLIPglu) Frommer Lab FLIPglu Plasmids Wolf Frommer Glucose FRET sensor to monitor...levels and transport (FLIIglu) Frommer Lab FLIIglu Plasmids Wolf Frommer Glucose Green fluorescent glucose...transport and metabolism (FLIPE) Frommer Lab FLIPE Plasmids Wolf Frommer Glutamate Ultrafast glutamate sensors...proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Red-, yellow-, or cyan-emitting homoFRET...using optical sensors. Biochem J. 2010 Dec 1. Wolf Frommer ATP yAT1.03 FRET-based ATP sensor for yeast An ...FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical distribution of the second messenger c-di-GMP...Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based maltose biosensor...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...James Trimmer 177520 Anti-LRRK2/Dardarin, C-terminus [N241A/34R] LRRK2 CMV Parkinson's James Trimmer 177537...Spinocerebellar ataxia James Trimmer 177546 Anti-PINK1 [N4/15R] PINK1 CMV Parkinson's James Trimmer 177653 pT7II-2N4Rtau...James Trimmer 182104 Anti-LRRK2/Dardarin, N-terminus [N138/6R] LRRK2 CMV Parkinson's James Trimmer 182113...Spinocerebellar ataxia 19 James Trimmer 188163 Anti-VAPA/B [N479/107R] VAPB CMV ALS James Trimmer 188167 Anti-IP3 receptor...ataxia James Trimmer 196946 Kv4.3 long/RBG4 KCND3 CMV Spinocerebellar ataxia 19 James Trimmer 197107 iYFP-Parkin...19 James Trimmer 206543 Anti-LRRK2/Dardarin, N3 [N231B/34R] LRRK2 CMV Parkinson's James Trimmer 206623 ... James Trimmer 206681 Anti-Kv1.1 K+ channel [K20/78R-1] KCNA1 CMV Episodic ataxia James Trimmer 206684...

  4. Antibody Production

    Type
    Collection
    ...Production & Usage” and “Delivery” conditions, so we recommend reviewing the specific antibody webpage for further...across a tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed...Storage and Shipping Stability Addgene does not recommend repeated freeze thaw of antibodies and suggests... against a non-treated control in one of its recommended assays. If an antibody has passed stability testing...

  5. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...genomic target, you need to make sure the NGG immediately follows your target on the genome but NOT on ...considerations only matter if the PAM sequence is immediately adjacent to the nearly-homologous target sites...work, do you have any suggestions? The Zhang lab recommends using the Epicentre QuickExtract solution to ... the second design (in pX330 / PX335 ), so we recommend people all use the pX330/PX335 system ....

  6. All Antibodies

    Type
    Collection
    ...with the antigen. Addgene supplies a list of recommended applications based on our in-house testing and...negative results when an antibody’s use is not recommended for an application or species. We currently assess...Description Source Species Isotype Reactivity Recommended Applications PI Return to Top Do you have suggestions...

  7. Institute for Protein Innovation

    Type
    Collection
    ...Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are...Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI...Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids The following...

  8. Genetic Code Expansion

    Type
    Collection
    ... 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl) Mammalian...Mehl 89189 pMaRSC methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl) Mammalian...Bacterial TAG Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry MetRS M. musculus azidonorleucine...

  9. Brain Initiative Collection

    Type
    Collection
    ...NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection . The NeuroMab Facility and James Trimmer have produced...antibodies ID Recombinant Antibody Reactivity Recommended Applications PI Additional Resources To learn...

  10. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...cgDNA, we hope to aid scientists who want to immediately start cloning upon arrival of their plasmid from...applications. Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning grade ...

  11. Resolute Plasmid Collection

    Type
    Collection
    ... Union's Horizon 2020 research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE...feedback form . If you have further questions or comments you can also contact them at [email protected]...

  12. AAVED

    Type
    Collection
    ...technology in the lab (e.g., controls to perform, recommended histology conditions, minimum/maximum incubation..., dose response data, list of antibodies and recommended dilutions, etc.), novel applications, or any ...

  13. AAV Packaged on Request

    Type
    Collection
    ...Overview Our new Packaged on Request service is recommended for research-grade use. Many chemogenetic, optogenetic...not perform functional or in vivo testing. We recommend that you consult the original paper of your chosen...

  14. CRISPR Guide

    Type
    Collection
    ...the rest of the genome The target is present immediately adjacent to a P rotospacer A djacent M otif (...well as additional DNA matching the sequence immediately upstream and downstream of the target site (termed...off-target mutations in DNA, RNA, or both, and are recommended in contexts where such mutations would be problematic.... T., Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C., Stan...

  15. CRISPR Plasmids - Tagging

    Type
    Collection
    ...endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated end-joining), an alternative...makes it easy to construct a donor vector, because MMEJ utilizes very short (around 20 bp) microhomologies...

  16. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...plasmid delivery before attempting genome editing. Immediately transfer solution from cuvette into 1 ml of culture... concentration. NOTE: While steps 6.1 and 6.2 recommend an efficient method for DNA extraction, any method...

  17. Bacterial Expression Systems

    Type
    Collection
    ...of N and C-terminal Strep and His tags Thorben Dammeyer Lactose/IPTG inducible vectors with LacIq, a stronger...FLIParaF.Ec-250n 18855 N/A FRET between CFP and YFP Wolf Frommer Reporter for arabinose levels in bacteria. The ...

  18. Tags and Other Markers

    Type
    Collection
    ...Description Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other...

  19. Neuroscience

    Type
    Collection
    ...Description Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other...

  20. Antibodies

    Type
    Collection
    ...antibodies and provide experimental data for recommended applications. Addgene values transparency and...
Showing: 1 - 20 of 39 results