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We narrowed to 113 results for: LEA

Showing: 21 - 40 of 113 results

  1. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...MDH). The MCC is a national group of educational leaders providing research opportunities to their students...MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the 6xHis-tag and the ... region of MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available... CUREs Community (Link opens in a new window) — Learn how to get started with a CURE, explore MDH projects...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...window) External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan...undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate...

  2. Retrograde AAV viral preps

    Type
    Collection
    ...CaMKII-somBiPOLES-mCerulean CamKII Bidirectional Optogenetics Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn Bidirectional...DREADDs Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825...GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting 51083 AAV-EF1a-DIO-...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Ting 83899...GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting 100853 pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system to facilitate genome...nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved in defense against invading phages...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease along with the guide RNA. For most applications...gain-of-function screening. For more information, please visit the Addgene SAM library page . Targeted gene... directed repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology... DNA targeting specificity of RNA-guided Cas9 nucleases. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. The robust signal, ...sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common choice in assays...have made it popular with scientists since its release. Luciferase-containing plasmids are commonly used...applications. Browse the Collection Highlights section to learn more. Highlights of Luciferase Tools in our Collection...artificial luciferase desined de novo using deep-learning approaches. Split-Luciferase and Protein-Protein...luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016...linker to the circularly permuted Venus fluorophore. Learn more about pHlash sensor in our 28 Hot Plasmid Technologies...

  5. COVID-19 Resources

    Type
    Collection
    ...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...entry into cells. ( 2 ) FURIN - an enzyme that cleaves precursor proteins to a biologically active state... The SARS-CoV-2 S protein contains a potential cleavage site for furin proteases.( 3 ) BSG - (CD147), ...function.( 5 ) TACE - (ADAM17) a metalloprotease that cleaves hACE2, potentially increasing viral infectivity...degradation to develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth...et al, 2008, J Virol. Cathepsin L Functionally Cleaves the Severe Acute Respiratory Syndrome Coronavirus...Heurich A, et al , 2014, J Virol. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2...

  6. CRISPR References and Information

    Type
    Collection
    ...analyze outcomes from cleaving nucleases (e.g. Cas9, Cas12a) and noncleaving nucleases (e.g. base editing...opens in a new window) A webtool that uses deep learning models for SpCas9 gRNA design and efficiency prediction...opens in a new window) A webtool that uses deep learning models for base editor gRNA design and efficiency...opens in a new window) A webtool that uses deep learning models for prime editor pegRNA design and efficiency...

  7. Rett Syndrome

    Type
    Collection
    ...protein function results in Rett syndrome is not clear, however, the ability to bind to methylated DNA ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic...receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not essential for localization...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...your plasmids! To get started with your deposit, please click here ....

  8. Optogenetics AAV Preps

    Type
    Collection
    ... 154948 CaMKII-somBiPOLES-mCerulean CamKII BiPOLES (soma-targeted) mCerulean Constitutive 5, 9, rg* Wiegert...154951 hSyn-DIO-somBiPOLES-mCerulean Syn BiPOLES (soma-targeted) mCerulean Cre dependent 5, 9, rg* Wiegert...Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...are also available Non-toxic to living cells To learn more about this system, visit the New England Biolabs...website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid...ACP-MCP-Tag plasmids - Substrate source (PDF, 46 KB) Please note: New England Biolabs (NEB) has discontinued...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted fluorescent...sensor (MARIO) A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically Encoded...3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin...

  11. Mammalian RNAi Tools

    Type
    Collection
    ...Dicer, an RNase III enzyme, binds the shRNA and cleaves it to produce siRNA. The siRNA then binds the RNA-induced... siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute ...argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows a similar mechanism...Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding of RISC. For more ...containing other RNAi components, like argonaute, please search our full site . ID Plasmid Vector Type PI...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...NUSA tag and TEV cleavage pExp-NusA - Expression of protein in E. coli as TEV cleavable N-terminal His ...of target gene with N-terminal GST tag and TEV cleavage Transient expression - Gradia Lab plasmids for...lab pFB-LIC-Bse - Insect expression vector from Gileadi lab Worm unc-54, variety of worm gene promoters...Guide and the Michael Davidson FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid...myc-nuc - pEntry vector that adds a C-terminal nuclear localization signal pGADCg - Gateway yeast expression...

  13. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Microtubules 87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20...114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP...NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding...fibrillar center) 133964 SON-mEGFP AICSDP-82 mEGFP SON Nuclear speckles 159744 DMD-mEGFP AICSDP-55 mEGFP Dystrophin...PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This...

  15. Control AAV Preps

    Type
    Collection
    ...Constitutive 9 Wilson 105557 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG CB7 mCerulean Constitutive 1, 9 Wilson 105598 pAAV.GfaABC1D.PI.Lck-GFP.SV40...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  16. Viral Production

    Type
    Collection
    ...each viral lot. To learn which specific QC experiments were performed on your lot, please contact us . Full...medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation and then by filtration...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...the sample from the DNA tube all the way to the cleared viral preparation pool. Proviral Identity Confirmation...

  17. Ras Pathway

    Type
    Collection
    ...NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide...guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2 RASGRP3 RASGRP4 RAS guanyl releasing protein RASSF...activating pathways for cell growth and potentially leading to cancer. Constitutively active Ras is the most...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCS2plus-mCerulean 156-239-GGGS-TDP-43 TARDBP mCerulean CMV ALS Marco Morsch 162618 pCS2plus-mCerulean 156-...Parkinson's, FTD Michael Davidson 55431 mCerulean3-MAPTau-C-10 MAPT mCerulean3 CMV Parkinson's, FTD Michael Davidson...Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His, mCerulean3 T7 Parkinson's Dan Mulvihill 110486...Lippincott-Schwartz 164214 PLEX-PGK-ANXA11-mCerulean ANXA11 mCerulean PGK ALS Jennifer Lippincott-Schwartz ...mRuby CMV ALS Jennifer Lee 216227 TDP-43 mCerulean TARDBP mCerulean CMV ALS Jennifer Lee 216843 pET-Duet_...If you do not see a particular gene of interest, please search our full plasmid collection using the search...Bacterial Yeast Other Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ...Disease. These diseases persist for many years and lead to a major burden on the patients, their families.... Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using...spinal cord. Over time, a loss of motor neurons leads to limb weakness, difficulty speaking, swallowing...percentage (5-10%) are familial ALS cases having at least one other affected family member. These familial... individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It...

  20. KLF Research Plasmids

    Type
    Collection
    ...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and shared... Krüppel-like factors (KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse...collaborative efforts within the KLF community have lead to the establishment of this shared resource on ...encourage you to contribute to this community resource. Learn about the benefits of depositing plasmids with Addgene...
Showing: 21 - 40 of 113 results