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We narrowed to 62 results for: Mammalian expression plasmids

Showing: 21 - 40 of 62 results

  1. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...CRISPR RNA targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ... that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does... and plants. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid... Plasmid Gene/Insert PI Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Addgene plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but...Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol for Genomic Deletions in Mammalian Cell Lines Other...cloning and expression purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ...constructs in which multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...Experiments (JoVE) about genomic deletions in mammalian cell lines using CRISPR/Cas9 CRISPR...

  3. Retrovirus Plasmids

    Type
    Collection
    ... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...envelope plasmid Nienhuis and Salmon 22501 pHEF-VSVG Envelope VSV-G-expressing envelope plasmid Kasparov Popular...γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral plasmid with your gene...10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses...

  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... CRISPR base editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants...zebrafish. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  5. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian ID ...ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... be added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...

  6. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Transposase plasmids. Plasmids are available for expression in mammalian systems and bacteria. Mammalian ID Plasmid...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base... Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... used in bacteria genome editing, though some mammalian systems have been developed. Figure 1: Overview...

  7. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS classic) sequences. Mammalian Targets...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein...time. Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified...a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that may be helpful...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...

  8. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria, Drosophila...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR... CRISPR Nick CRISPR Plasmids: Nick Browse...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

  9. Adenovirus Plasmids

    Type
    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral...viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...homologous recombination of adenovirus plasmids in either mammalian cells or microorganisms, including bacteria...sequences from both plasmids. This DNA molecule can then be transfected into mammalian packaging cell lines...

  10. Plan Your Experiment

    Type
    Collection
    ...dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ... your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...Delivery Using Plasmids The most straightforward method to deliver a CRISPR system into mammalian cells is ...is to directly transfect expression plasmids encoding your Cas protein and gRNA, which you generally perform...electroporation. Expression can be transient, or you can generate stable cell lines if your plasmid contains ...

  11. Antibody Production

    Type
    Collection
    ...encoded by mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified from the supernatant...retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ... SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase...., Science 2013). Additionally, we have a plasmid expressing SpCas9 alone without sgRNA. Full references...nickase) + single guide RNA: These plasmids contain two expression cassettes, a human codon-optimized ...sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9...

  13. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression in mammalian systems...Xenopus. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that create double strand breaks in DNA. CRISPR...

  14. CRISPR Plasmids and Resources

    Type
    Collection
    ...Drosophila . Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety .... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in...

  15. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...please see our Plasmid Distribution to Industry page. These plasmids have not been expressed or functionally... enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene has partnered...Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based...OC43, NL63, HKU1, and 229E) Expression type (e.g. bacterial, yeast, or mammalian) Tags (e.g. 6xHis, Flag,...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems for a summary of the Ginkgo ...Browse plasmids encoding SARS-CoV-2 viral proteins. Depositor...

  16. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA... gRNA expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a ...transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should be added to...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...

  17. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select...Select a gRNA expression plasmid based on factors such as selectable marker or cloning method. When using...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a ... Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...

  18. Brain Initiative Collection

    Type
    Collection
    ...Chemogenetics expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...driven KOR DREADD expression. Bryan Roth 75033-AAV1 pAAV CD68-hM4D(Gi)-mCherry Expression of hM4D(Gi) using...production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden...axon-GCaMP6s-P2A-mKate2 For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2 Lin Tian 112005...axon-GCaMP6s-P2A-mRuby3 For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3 Lin Tian 112005...axon-GCaMP6s-P2A-mRuby3 For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3 Lin Tian 112006...

  19. Plasmid Collections

    Type
    Collection
    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 161,273 plasmids . We've...Inducible Expression Worm Expression Kits Kits are groups of plasmids that have been packaged together because...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Expansion Immunology Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible...

  20. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a ...Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid Calarco... need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912...
Showing: 21 - 40 of 62 results