Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 47 results for: abo.1

Showing: 21 - 40 of 47 results

  1. Brain Initiative Collection

    Type
    Collection
    ...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV5 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV8 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV9 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-AAVrg pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-PHPeB pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  2. CRISPR Guide

    Type
    Collection
    ... most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism Engineered...from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while retaining the same basic...lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in the gRNA-containing...efficiency and fidelity. Nature Communications , 13 (1), 1425. PMID: 35301321 Edraki, A., Mir, A., Ibraheim...interrogation by SpRY-Cas9. Nature Communications , 15 (1), 3663. PMID: 38688943 Hsu, P. D., Scott, D. A., Weinstein...increase its specificity. Nature Communications , 9 (1). PMID: 30082838 Maddalo, D., Manchado, E., Concepcion...CRISPR-Cas9 with Bacteriophage Proteins. Cell , 168 (1–2), 150-158.e10. PMID: 28041849 Sakuma, T., Nishikawa...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6...SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide ...screens. The libraries are available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together...):186-91. doi: 10.1038/nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids...Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...

  4. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...effects in vivo . While AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled...Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid, expressing Rep2 and Cap1...production. Read our AAV Guide for more information about: AAV components Common uses of AAV Viral integration...out our Viral Vector Packaging Service and learn about our options for hundreds of in-stock preps that ...

  5. Plan Your Experiment

    Type
    Collection
    ...sequences containing a G nucleotide at position 20 (1 bp upstream of the PAM) may be more efficacious than...population. Some cells may be wild type due to either (1) a lack of gRNA and/or Cas9 expression or (2) a lack... in vitro and in vivo. Nature Biotechnology . 33 (1), 73–80. PMID: 25357182...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable...when carrying out CRISPR experiments. Read more about how to design your gRNA . Browse Plasmids: gRNAs...to optimize your delivery conditions. Learn more about CRISPR delivery in mammalian systems . Validate ...

  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...donor and acceptor. FRET is often used to study (1) protein-protein interactions where each protein is...standard consisting of ECFP and EYFP connected by 1 flexible glycine- and serine-containing peptide linker...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins ...

  7. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...for any application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable...adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye, et al. 2015. Safety and...Information The plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid...

  8. MAPK Plasmids

    Type
    Collection
    ... PRKM1, PRKM2, p38, p40, p41, p41mapk MAPK3 ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK,... point for multiple biochemical signals. In collaboration with numerous research labs from around the ...

  9. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression...attachment site of the phage 186. Detailed information about the repression system can be found in this article...29519933 . bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in...

  10. Resolute Plasmid Collection

    Type
    Collection
    ...and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics and genome engineering...

  11. Rett Syndrome

    Type
    Collection
    ...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development... the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...contact information by following the link to their laboratory website. Mouse Line Mutation (DNA) Background...contact information by following the link to their laboratory website. Cell Line Mutation (DNA) Mutation (protein...

  12. Distribution to Industry

    Type
    Collection
    ... your account. We will notify you by email within 1 business day regarding your account verification. ...plasmids that are available to industry. Learn more about the available plasmids and how to order them. Distribution...Information page for more information. To learn more about Addgene’s MTA process please visit our Help Center...

  13. Caltech Systemic Capsids

    Type
    Collection
    ... Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. Your ...tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2...systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in ...AAV Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your cell type...

  14. Antibody Production

    Type
    Collection
    ...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible for...quality control partner before distribution. Details about our production protocols, titering methods, and ...Depositing Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for...

  15. AAVED

    Type
    Collection
    ...11:00 AM Circuit Tracing Ali Cetin 12:00 PM Lunch 1:00 PM Promoter/Enhancer Specificity, Serotype specificity...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series. The... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research...

  17. Viral Vectors

    Type
    Collection
    ...material to specific cells in an organism. Figure 1. Using viral plasmids to generate virus. A) Viral ...Virus Should I Use? Use this guide to learn more about the different types of viruses that are commonly...temporary in the host. Use this guide to learn more about the different types of viruses that are commonly...found in the guides for each virus type, linked above. Virus Expression Genome Packaging Capacity Virus...

  18. TALEN Guide

    Type
    Collection
    ...coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease...researchers who are learning to wield them. Learn more about Addgene’s TAL effector and TALEN kits here . References...

  19. Viral Production

    Type
    Collection
    ...to determine purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV ... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...

  20. Neurodegeneration Research Collection

    Type
    Collection
    ...one of three different inherited genes: Presenilin 1, Presenilin 2, and APP gene.The majority (>90%) of...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using CRISPR...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...
Showing: 21 - 40 of 47 results