We narrowed to 58 results for: abo.1

...changing the target sequence present in the gRNA. Figure 1: Overview of the basic CRISPR mechanism. CRISPR was...from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while retaining the same basic...lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in the gRNA-containing...efficiency and fidelity. Nature Communications , 13 (1), 1425. PMID: 35301321 Edraki, A., Mir, A., Ibraheim...interrogation by SpRY-Cas9. Nature Communications , 15 (1), 3663. PMID: 38688943 Hsu, P. D., Scott, D. A., Weinstein...increase its specificity. Nature Communications , 9 (1). PMID: 30082838 Maddalo, D., Manchado, E., Concepcion...CRISPR-Cas9 with Bacteriophage Proteins. Cell , 168 (1–2), 150-158.e10. PMID: 28041849 Sakuma, T., Nishikawa...

... . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin...for hygromycin selection. Bob Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid...coexpression. Didier Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression...our Lentiviral Vector Guide for more information about lentiviral components, generations, viral production...

...enzyme and guide RNA) for your experiment (Figure 1). You will decide how to express Cas9, the delivery...finally how to validate your genetic edit. Figure 1: Flow chart describing the general framework of a ...population. Some cells may remain wild type due to either (1) a lack of gRNA and/or Cas9 expression or (2) a lack...Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit, A., Parnas, O...exon coding for an essential protein domain could abolish protein activity and essentially function as a ...reference sequence you used for gRNA design. Read more about how to design your gRNA . Synthesize and Clone Desired... other downstream methods. For more information about viral vectors and their production, see our viral...

..., or the beginning of the coding sequence. Figure 1: Overview of CRISPR interference. Browse, sort, or...for CRISPR interference plasmids. To learn more about CRISPR interference and other CRISPR topics, read...

...small enough to be packaged in AAV particles. Figure 1: Overview of RNA targeting with CRISPR. Browse, sort...plasmids designed for RNA editing. To learn more about RNA editing and other CRISPR topics, read our CRISPR...

...also indirectly modify local chromatin state. Figure 1: Overview of epigenetic modification using CRISPR....designed for epigenetic modification. To learn more about epigenetic modification with CRISPR and other CRISPR...

...for any application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable...adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye, et al. 2015. Safety and...Information The plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid...

...the dCas9-activator to your specific locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com...below for CRISPR activation plasmids. To learn more about CRISPR activation and other CRISPR topics, read ...

... PRKM1, PRKM2, p38, p40, p41, p41mapk MAPK3 ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK,... point for multiple biochemical signals. In collaboration with numerous research labs from around the ...

... edits via homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse...below for CRISPR nickase plasmids. To learn more about CRISPR nickases and other CRISPR topics, read our...

...collateral RNA degradation seen in bacteria. Figure 1: Overview of RNA targeting. Browse, sort, or search...plasmids designed for RNA targeting. To learn more about RNA targeting and other CRISPR topics, read our ...

...with a genomic region of interest in vivo. Figure 1: Overview of purification of genomic loci using CRISPR...the purification of genomic loci. To learn more about purification using CRISPR and other CRISPR topics...

...Expression mEGFP 488 507 34 6 Monomer (A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression...TurboRFP-pBAD - Bacterial Expression tdTomato 554 581 95 4.7 1 h Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...-pBAD - Bacterial Expression mRFP1 584 607 13 4.5 1 h Monomer pcDNA3-mRFP - Mammalian Expression pMXs-...Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo...pSNAPf-C1 - Mammalian Expression pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf...colors as needed for your experiments. Learn more about the flexibility of self-labeling tags on our blog...

...Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression...attachment site of the phage 186. Detailed information about the repression system can be found in this article...29519933 . bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in...

...with target sites throughout the chromosome. Figure 1: Overview of visualizing genomic loci using CRISPR...the visualization of genomic loci. To learn more about visualization using CRISPR and other CRISPR topics...

...occurs at a much lower efficiency than NHEJ. Figure 1: Overview of NHEJ and HDR following cleavage by Cas9...plasmids designed to introduce a DSB. To learn more about NHEJ, HDR, and other CRISPR topics, read our CRISPR...

...some mammalian systems have been developed. Figure 1: Overview of the basic CAST mechanism. Created with... for CRISPR transposase plasmids. To learn more about CRISPR transposases and other CRISPR topics, read...

...and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics and genome engineering...

...for brain circuit mapping and connectomics. Figure 1: Reconstruction of a sparse set of neurons across ...Learn about PRISM, a set of molecular tools for high-throughput reconstruction of individual neurons ... Research (Link opens in a new window) , in collaboration with The Francis Crick Institute (Link opens...Link opens in a new window) . For more information about PRISM, see the preprint: Park, S.Y., Sheridan, A...

...activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing. Browse, sort, or...for CRISPR base editing plasmids. To learn more about base editing and other CRISPR topics, read our CRISPR...