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...For more details on protocols see the Addgene Protocols page or the Neuromab Protocols (Link opens in a ...then purified from the supernatant using Protein A or Protein G affinity columns. Formulation Buffer After...measured and the ratio of the antibody protein content to the total protein content of the sample is determined...secondary antibody. Protein expression is compared to an untransfected control and the protein size is confirmed...before distribution. Details about our production protocols, titering methods, and quality control are described...by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies, IPI will...these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage. Antibody...

... DNA repair protein, and is suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag...complementary protein function or localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ...ACP or MCP tagged plasmids for visualization of proteins inside cells. Depositor...Information New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ACP-, and... and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated with...features: Allows for visualization of multiple proteins simultaneously with dual labeling when used with...

...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Engineering You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly after where the... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types...

... Antibodies in Basic Neuroscience Research. Curr Prot Neurosci . 94.1. PMID: 33151027 (Link opens in a...Camelid-Derived Nanobodies for Neuroscience Research. Curr Prot Neurosci . 94.1. PMID: 33185319 (Link opens in a...Gamma-protocadherin-A3 [N144/5R] Gamma-protocadherin-A3 Mouse Mouse IgG2a 222155 Gamma-protocadherin-B2 ...ATPase 1 (Menke's disease protein) [L60/4R] Copper ATPase 1 (Menke's disease protein) Human Mouse IgG2a 114503...Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R] MMP9 matrix metalloproteinase-9 precursor Rat ...Mouse IgG2a 164156 Anti-Gamma-protocadherin-C3 [N174B/27R] Gamma-protocadherin-C3 Mouse Mouse IgG2a 164157... 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504...

... CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen... a new window) . Protocols The Allen Institute for Cell Science has several protocol videos available ...deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human cell lines... will be able to generate fluorescently tagged proteins of key cellular structures in human cell lines...structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin...87429 TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP-35...MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related protein LC3 B Autophagosomes 101784 SLC25A17-mEGFP AICSDP...

...manipulating, and evolving proteins. Expanding the Genetic Code During protein translation, an aminoacyl-tRNA...amino acids within a protein, any alterations in structure or function in the protein can be observed. The...intentionally change the activity of a protein (e.g. converting a DNA binding protein to a DNA cleaving enzyme) or...express the protein with and without the ncAA in the media to make sure that the full length protein is only...phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein. Ryan Mehl...the genetic code and incorporate a ncAA into a protein of interest, four key modifications to the standard...the media can be incorporated using the existing protein translation machinery. A synthetase plasmid expressing...

...information (Link opens in a new window) UniProt MECP2 - MECP2 curated protein and functional information Mouse...variability (Link opens in a new window) Protocols.io - protocol sharing site Funding Opportunities (Link...gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts of this disease...disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2 ). MECP2 Rett syndrome is an X-linked... ratios can affect disease severity. The MECP2 protein is a global transcriptional regulator of thousands..., gene activation, and more. How loss of MECP2 protein function results in Rett syndrome is not clear,...expression either locally or globally. The MECP2 protein contains several functional domains, including:...

...three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression in ... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection is organized...microbe of interest, including bacteria, viruses, protozoa, fungi, and more. Plasmid...including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below for the species...Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium...and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz...

...Damage-specific DNA binding protein 2, 48kDa p53 Tumor protein p53 p53AIP1 Tumor protein p53 regulated apoptosis...factor binding protein 3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene, E3 ...angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid BH3 interacting domain death agonist CASP3 ...finger and WD repeat domain 2 (RFWD2); E3 ubiquitin protein ligase cytoC Cytochrome c DR5 Tumor necrosis factor...E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced...acetate-induced protein 1; also known as PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent...apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger...

...Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...2014) (Link opens in a new window) . protoSpaceJAM protoSpaceJAM is an all-around platform for CRISPR ...cloning; injection protocol pU6-BbsI-chiRNA ; phsp70-Cas9 PDF, 109 KB Orkin and Bauer Protocol for Genomic Deletions...Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's...genomes, including animals, plants, bacteria, fungi, protists, and viruses. Developed by the Joachim Wittbrodt... (Link opens in a new window) A collection of protocols, materials, and publications by members of the...

...capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction of total protein present...electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total protein present in the sample...distributed to customers. Details about our production protocols, titering methods, and quality control are described...elements and an internal control of known titer (protocol modified from Lock et al. (2014) (Link opens in...vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC). ...by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties of the...relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...

...and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein. EnvA-pseudotyped...starter cell using the G-protein, which is supplied in trans . (3) Nascent G-protein coated RAVB is released...infected with G-protein coated RABV. These projection neurons do not express G-protein, and thus do not...For example, G-deleted rabies lacks the viral glycoprotein (G) gene, which is not required for the transcription...of this deletion-mutant rabies, the essential G protein is provided to the starter cell in trans , via ...the secondarily infected cells that lack the G protein, the virus can replicate but the newly formed viruses...neuronal population. This is done using the envelope protein of (EnvA) of leukosis virus. EnvA can direct virus...

...associated protein; LST8 homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1...known as MAPKAP1; Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin...gamma PKC eta PKC iota PKC theta PKC zeta Protein kinase C Protor Also known as PRR5; proline rich 5 PTEN...to catabolic processes. Active mTORC1 promotes protein and lipid synthesis, and increases energy production...Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic...initiation factor 4E GBL/mLST8 MTOR associated protein; LST8 homolog LKB1 Also known as STK11; serine/...Raptor Also known as RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in...

...misfolding and aggregation and can interfere with protein-protein interactions and nerve cell function within...highly conserved proteins and other difficult-to-target antigens in the Institute for Protein Innovation Antibody...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including Markers...Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative...curing and treating them. Scientists are studying protein aggregation, learning about the blood brain barrier...with the mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. ...environmental factors. For example, variations of Apolipoprotein E (APOE), such as the ε4 allele, are a risk...

...it to pull out a protein of choice from a mix of other proteins, visualize a protein under a microscope...synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger Antibody Plasmids The...Antibodies Antibody Plasmids Antibodies are large proteins made by the immune system that bind to an antigen...microscope, or detect when the protein is present in a sample — just to name a few. Traditional sources of antibodies... Antibodies: Selecting and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology...against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining. Biochem...synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies for...

...amino acids) fragments for protein-protein interaction assays. Each target protein is fused to either LgBiT...Luciferase Complementation assays can report on protein-protein interactions, drug-induced changes, and more...Both firefly and Renilla luciferase have short protein half-lives, making them useful as transcriptional...transcriptional reporters, but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively...ATP-independent, and a secreted version of the protein is available, making it ideal for a variety of ... BRET on our blog . Nano-Lanterns: Fluorescent proteins fused to Renilla luciferase ( Nano-lanterns ) ...Engineered circular RNAs for enhanced NanoLuc® protein production. Other Sensors and Tools Optimized STARR-seq...

...encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru 104055...genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055...genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104061...eYFP An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter Viviana Gradinaru ...

...types of proteins can also be controlled by light, generally by utilizing a light sensitive protein from ...light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as an optical switch to control protein interactions...Additional Resources Optogenetics plasmids encode proteins that are controlled by light. Many of these plasmids... opened and closed in response to light. These proteins can be expressed in specific neurons, often utilizing...activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression and...localization or activity of many different types of proteins. For a full description (including peak response...below for plasmids that use light to control a protein or cellular activity. For example, search "Cdc42...

...to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection....Pooled Libraries Feedback Resources Solute carrier proteins (SLCs) are the largest family of transporters ...core of the deorphanizing engine. Genetic and proteomic interactions represent the framework that provides...impedance, fluorescence, mass/charge properties, protein thermal stabilization, and changes of voltage all...contribute to assess ligand action/engagement. Protein production is used for assays and to generate antibody... libraries target the solute carrier family of proteins. Library ID PI Description Human SLC Activation... CRISPR activation library targeting human SLC proteins. Human SLC Knockout Library 132552 Superti-Furga...

...fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast....create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are...collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive... and KANMX markers. The FP together with the protothropic cassette can be amplified with the following...Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources Teusink...2019). In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https:/...2013) Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 ...