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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 71 results for: CaS

Showing: 41 - 60 of 71 results

  1. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure 1: Overview...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  2. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...markers. The FP together with the protothropic cassette can be amplified with the following primers: FW...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials... Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces...., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk Parijs ...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan Kissler... plasmid with a chimeric 5’LTR. Any expression cassette (promoter and gene of interest) can be cloned ...interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). Find...

  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...modifications, researchers have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  5. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

  6. CRISPR Plasmids - Parasites

    Type
    Collection
    ...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...Patrick Caswell 179900 pJET-59-DExCon-mTagBFP2-R25 RAB25 mTagBFP2 TRE3GS Parkinson's Patrick Caswell 180016...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy...pEGFP-C9orf72 C9orf72 GFP CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...Wernig 176852 UBQLN2_450-624W UBQLN2 T7 ALS Carlos Castaneda 176915 pcDNA3.1(+)_Paragranulin+linker 1 GRN Flag...

  8. Antibody Guide

    Type
    Collection
    ...expression. Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope...when epitope specificity is not a concern or in cases where you may want to identify or collect the protein...azide, as it acts as an inhibitor to HRP. In those cases, thimerosal can be used as an antimicrobial agent... using the above process and try again. In some cases, it may be preferable to buy small aliquots of many...

  9. The Pleiades Promoter Project

    Type
    Collection
    ...webpages. These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters MiniPromoter...promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson, D. J., Liu, L., de Leeuw, C. N., ...Bonaguro, R. J., Brauer, E., Candido, T. R., Castellarin, M., Chen, J., Chen, Y., Cheng, J. C., Chopra...

  10. Bacterial Expression Systems

    Type
    Collection
    ...protein expression in the stationary phase. In these cases, you may want to control protein levels or to turn...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...synthesize a target metabolite, genetic tools like CRISPR-Cas9 are not only valuable but often essential. Find ...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice...

  12. Brain Initiative Collection

    Type
    Collection
    ...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can...

  13. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...titered by droplet digital PCR (ddPCR) . In some cases, we may expect lower titers for a particular AAV...titer due to toxicity or other reasons For these cases, Addgene will promptly notify you by email within...

  14. COVID-19 Resources

    Type
    Collection
    ...19 research, including: CRISPR tools, like Cas12a and Cas13a plasmids, to reliably detect nucleic acids...

  15. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

  16. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

  17. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

  18. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

  19. CRISPR Plasmids - Plants

    Type
    Collection
    ...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

  20. CRISPR Plasmids - Yeast

    Type
    Collection
    ...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...
Showing: 41 - 60 of 71 results