...constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization ... PGK1 promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional ... Vickers CE, Bydder SF, Zhou Y, Nielsen LK. Dual gene expression cassette vectors with antibiotic selection...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter Tags PI Publication ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based...interactions. Plasmids A diverse set of wild-type MDH genes from several species and mutant clones from watermelon..., or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs ...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...this table based on plasmid name, gene/insert, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...of drug targets in Alzheimer’s disease (AD) by generating validated resources for emerging targets in AD...IUSM-Purdue TREAT-AD centers will be deposited at Addgene and available to request. Plasmids Browse the table...

...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and... pathways and auxiliary components. Networks and Gene Regulation Browse plasmids containing both naturally...Lab RNA Feedback Lu Lab Multiplexed/Programmable Gene Networks Mascher Lab Bacillus BioBrick Box Mukhopadhyay...about synthetic biology resources available at Addgene. Browse featured collections or depositing labs...engineering of biological systems for novel functions. Addgene is working with leading synthetic biologists around...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...signaling and environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including...

...Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized Pool...Keith Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...transfer plasmids. Want a retroviral plasmid with your gene of interest? Search our database for retroviral ...Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen for ...'s genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets integrated...to ∼8 kb), and infection results in long-term transgene expression. Some disadvantages of γ-retroviruses...

...zebrafish genes; many of these were generated as part of an NIH-sponsored effort to provide gene-editing...zebrafish genes. TALengineering... TALengineering Reagents TALEN Zebrafish Reagents TALENs for Endogenous Zebrafish Gene Targets...gene-editing nucleases to the zebrafish community. Target Gene Name TALENs Full Target Site (5' to 3'; half-sites...

...possible deletion strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located...evaluate the RNA, perform RT-qPCR for gene expression of the relevant gene 7,8 . To evaluate the protein, perform...genome editing tool that allows genetic perturbation of genes and genetic elements. Here we present a simple...for efficient loss-of-function studies of genes and genetic elements in mammalian cell lines. Protocol...theory and methods, perspectives for drug delivery, gene therapy and research. Gehl J. Acta Physiologica...Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present... be utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include...

...deposit@addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene Alternate... numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9...CRISPR screens targeting a long or short list of genes provided by the user. Developed by the Fredrik Wermeling...sgRNA design tool for genome editing, as well as gene regulation (repression and activation). Genome support...target sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Includes a wide range... to identify novel gRNA target sites in a single gene, as well as a target site common to a set of similar... Information Addgene Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR...

...transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors...10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is... transcription factor collection, available at Addgene. The collection can be used for screening transcription... entire pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...table based on plasmid name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication ...cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem Cell Research...against p53 in different gene/insert combinations A more efficient method to generate integration-free human...non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Mar...human cells to pluripotency by activating enogenous genes Human pluripotent reprogramming with CRISPR activators...S1934-5909(19)30423-0. Schöler You can also find gene-specific plasmids at the following links: NANOG ...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...

... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human...Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window... The plasmids used to generate these lines have been deposited here with Addgene for distribution to the...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular ...With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular ... with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...

... AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter..., "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication Return...Collections Optogenetics Plasmids Optogenetics Plasmid Collection You may also like... Optogenetics Guide AAV...tables, refer to Addgene's Optogenetics Guide . Interested in more news about optogenetics research? Find... AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial...Find optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate... Plasmids Optical Switch Plasmids Background Optogenetics plasmids encode proteins that are controlled...

... lists gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that...the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions...gRNA expression vectors available from Addgene. CRISPR...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other... effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence designed...promoters. Do you have gRNAs you'd like to add to the Addgene collection? Click here to start the deposit process...plasmids added to the list. You can also email help@addgene.org for more information and assistance with the ...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...