...after the promoter, and so on. The following table provides information for the assembly standards most commonly...will need to be reverse complimented for primer design. The restriction enzymes used for assembly are ..., NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare...Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix start codon; Employs...

...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...Parkinson's Disease; For target validation and drug design Antibodies Addgene distributes ready-to-use recombinant...materials and intellectual property rights, and provides liability protection for material providers. Addgene...

...material (termed the viral genome) that, in general, encodes for more viruses. When a virus infects a host cell.... Which Type of Virus Should I Use? This table provides a brief overview of the properties of different.... More detailed information can be found in the guides for each virus type, linked above. Virus Expression...Core . Viral Plasmid Biosafety Resources The NIH provides basic guidelines for working with various types...

...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA...the particular conditions of the experiment as described in the associated article (listed below by PubMed...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page... to indicate the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable...

... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link...

...recently described a rapid and robust protocol for constructing TALE repeat arrays of any desired sequence...Joung Lab REAL Assembly TALEN Kit , which also includes the four TALEN expression vectors. More detailed...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted epigenetic...catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9...gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems,...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require...encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully functional CRISPR...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...or search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for...

...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...

...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...

...laboratory for engineering TAL effectors, including designed TALENs, and assembly methods for REAL, REAL-Fast... engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation...for FLASH assembly. Links to additional pages describing these reagents are provided below: Joung Lab ...

...proteomic interactions represent the framework that provides biological context. Assays monitoring impedance...sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base editors...protein (Mu- GAM). While many base editors are designed to work in a very narrow window proximal to the...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...protein with many available isoforms and mutants provides a shared experience for the research community...site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH...Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP et al. CBE Life Sci Educ...

...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...