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We narrowed to 28 results for: PAM

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Showing: 1 - 20 of 28 results

  1. CRISPR Guide

    Type
    Collection
    ...enzymes with altered PAM specificities are available, known as PAM-flexible or PAMless Cas9s. SpCas9-derived...adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG) serves as a binding signal for...Increasing Cas9 PAM flexibility CRISPR systems designed for more precise edits require PAM sequences in ...region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant throughout the human...= C or T SpRYc — SpRY PAMs plus NNG; a chimeric version of SpRY Though these PAM-flexible Cas9s are useful...TTTV (V = A, C, or G) PAM sites. Engineered versions of Cas12a recognize different PAM variants, with one...of Cas Enzymes Cas Enzymes and PAM Sequences Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes...

  2. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with alternative PAM sites . Cpf1 , another CRISPR nuclease, recognizes a 5’-TTN PAM, improving targeting... sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and...it contains a special Protospacer Adjacent Motif (PAM) directly after where the crRNA would bind. (5) After...stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after...bacterial genomes, are highly diverse, with varying PAM sequences and number/types of Cas proteins. Makarova...packaged in AAV. Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple ...2015. Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature . 523(7561):481-5. PMID: 26098369...

  3. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...to uridine within a small editing window near the PAM site. Uridine is subsequently converted to thymidine... to work in a very narrow window proximal to the PAM sequence, some base editing systems create a wide...

  4. Plan Your Experiment

    Type
    Collection
    ... no PAM site close to your desired edit site. Consider alternative Cas9 enzymes with different PAM sequences...activity within a very narrow window proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions...sequences or Cas9 enzymes with flexible PAM sites . Base Editing : Target the site of your desired edit...their functions within a limited window from the PAM sequence, and base editor variants often have different...

  5. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...sequence identity to the spacer sequence and contain a PAM sequence just downstream of the sequence to be cleaved...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ...and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found that adding an additional...and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 alone: This plasmid contains ...need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have found that adding an additional...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...MAPT Flag CMV Parkinson's, FTD Stefan Stamm 194242 pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA SNCA HA SAR-CAG ...Parkinson's Michael J Fox Foundation MJFF 194247 pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA SNCA GFP CAG...Parkinson's Michael J Fox Foundation MJFF 194248 pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA SNCA GFP CAG...dementia (FTD) Thomas Kukar 213697 pLenti HsATP13A2 WT (PAM) _ Hygro ATP13A2 CMV Parkinson's, Kufor-Rakeb syndrome...226643 pAM219 VCP His T7 ALS Andreas Martin 226644 pAM220 VCP His T7 ALS Andreas Martin 226645 pAM221 VCP...226646 pAM222 VCP His T7 ALS Andreas Martin 226647 pAM224 VCP His T7 ALS Andreas Martin 226651 pAM229 VCP...T7 Huntington's Pamela Bjorkman 11514 pET32a-HD39Q HTT His, Trx T7 Huntington's Pamela Bjorkman 11515 ...

  10. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression PAmCherry1 564 595 405 8 6.3 23 min Monomer pPAmCherry1-N1 - Mammalian Expression pPAmCherry1-C1 -.../HisB-PAmCherry1 - Bacterial Expression PAmCherry2 570 596 405 13 6.2 18 min Monomer pPAmCherry2-C1 - ...Expression PAmCherry3 570 596 405 5 6.2 25 min Monomer pPAmCherry3-C1 - Mammalian Expression PAmKate 568 628...405 5 5.6 19 min Monomer pPAmKate-N1 - Mammalian Expression pBAD/HisB-PAmKate - Bacterial Expression PAiRFP1...

  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...monitoring dopaminergic activity in vivo. Nat Methods. 2023 Nov 30. Yulong Li Dopamine Dopamine sensors ...2020.02.07.939504 Loren Looger , J. Julius Zhu Dopamine Red and green GRAB_DA3 dopamine sensors Improved green and red...monitoring dopaminergic activity in vivo. Nat Methods. 2020 Nov;17(11):1156-1166. Yulong Li Dopamine Ultrafast...neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine dynamics with ...science.aat4422. Lin Tian Dopamine Yellow or Red genetically encoded dopamine sensor YdLight1 and RdLight1...RdLight1 An expanded palette of dopamine sensors for multiplex imaging in vivo. Nat Methods. 2020 Nov;17(...

  12. Brain Initiative Collection

    Type
    Collection
    ...pAAV-hSyn-GRAB_DA1m express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons Yulong Li 113050...pAAV-hSyn-GRAB_DA1h express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 113050...pAAV-hSyn-GRAB_DA1h express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 115892...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125560-AAV5 pAAV-CAG-dLight1.3b...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125560-AAV9 pAAV-CAG-dLight1.3b...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125707-AAV9 pAAV-CAG-RdLight1...pAAV-CAG-RdLight1 Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708...

  13. mTOR Pathway

    Type
    Collection
    ...Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling...threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1...associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53 PDK1 Pyruvate dehydrogenase...10.1016/j.cell.2012.03.017. PubMed PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis ...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons...Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion...conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...31930 pPAmCherry-a-tubulin Microtubules alpha-Tubulin PAmCherry Vladislav Verkhusha 31949 pPAmCherry-b-actin...-actin Actin filaments beta-Actin PAmCherry Vladislav Verkhusha 79990 pKeratin-miRFP703 Intermediate filaments...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...primarily involves the malfunction and death of dopamine-producing neurons in the substantia nigra, one...indicators . (Aggarwal et al. bioRxiv. 2025) Monitor dopaminergic activity in vivo with improved green and red... Acad Sci USA. 2017) Reprogram astrocytes to dopaminergic neurons using three transcription factors in...

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed...Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate neuron projections to the nucleus accumbens...2018. Mapping projections of molecularly defined dopamine neuron subtypes using intersectional genetic approaches...

  18. Retrograde AAV viral preps

    Type
    Collection
    ...genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor Yulong Li 123308 pAAV-hSyn-GRAB_NE1m...GECO Other Biosensors Glutamate Norepinephrine Dopamine Optogenetics Activators Inhibitors Bidirectional...

  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Botch Rat Mouse IgG2a 114488 Anti-Dopamine D3 receptor [N331/19R] Dopamine D3 receptor Human Mouse IgG2a ...NALCN Rat Mouse IgG2a 177509 Anti-Dopamine D2 receptor [N186/29R] Dopamine D2 receptor Rat Mouse IgG2a 177510...Gamma-protocadherin-B2 Mouse Mouse IgG2a 222156 Dopamine D2 receptor [N186/26R] Dopamine D2 receptor Rat Mouse IgG2a 222157...

  20. Biosensor AAV Preps

    Type
    Collection
    ...Sensors iAChSnFR GRAB_ACh Adenosine Sensors GRAB Ado Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA GRAB_gDA...HaloCaMP1b EGFP Constitutive 1, 9 Eric Schreiter Dopamine Sensors 111066 pAAV-hSyn-dLight1.1 Syn dLight1.1...
Showing: 1 - 20 of 28 results