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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 40 results for: dcas9, grna

Showing: 1 - 20 of 40 results

  1. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter...

  2. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding known ...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding known ...

  4. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or ... Biotin tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing BirA...CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known ...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying molecules...of any genomic sequence specified by a particular gRNA. In the enChIP (engineered DNA-binding molecule-...or gRNA for efficient purification. Various epitope tags including 3xFLAG-tag, PA, and biotin tags, can...

  5. CRISPR Plasmids - Plants

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...

  6. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR dCas9-FokI CRISPR: dCas9-FokI...Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI...specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...

  7. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer...

  8. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...

  9. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...

  11. CRISPR Guide

    Type
    Collection
    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent... specified by a particular gRNA. Epitope tag(s) are fused to dCas9 or gRNA for efficient purification ...CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator with a gRNA to increase transcription of a target...CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA to repress/decrease transcription... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...

  12. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Preps Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive Cas9 (dCas9) fused to a ...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...fluorescent marker like GFP, researchers have turned dCas9 into a customizable DNA labeler compatible with ...fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers...it is easy to implement due to the simplicity of gRNA design, programmable for different genomic loci,...efficiently visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity...

  13. Plan Your Experiment

    Type
    Collection
    ...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be...edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit. Science... system for Cas9 and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different ... proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...your gRNA is the balance between on-target and off-target activity. In a perfect world, your gRNA sequence...

  14. CRISPR Plasmids and Resources

    Type
    Collection
    ...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment...experiment based on a variety of factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  15. Lentiviral Prep Service

    Type
    Collection
    ...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A ...RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting...dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A ...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ... Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...57,050 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. 57,050 unique...57,011 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. 57,011 unique...

  16. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted ...

  17. CRISPR References and Information

    Type
    Collection
    ...at Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 ...DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF,...activation dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator...Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized ...optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo...

  18. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic parasites...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the... - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158...

  19. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...genome. gRNA Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector... Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned...Empty gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and a Cas ...pyogenes Puro Zhang phH1-gRNA 53186 Mammalian BbsI none S. pyogenes Gersbach pmU6-gRNA 53187 Mammalian BbsI...pyogenes Gersbach phU6-gRNA 53188 Mammalian BbsI none S. pyogenes Gersbach ph7SK-gRNA 53189 Mammalian BbsI...

  20. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base... nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within a small...
Showing: 1 - 20 of 40 results