Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 44 results for: grna design

Showing: 1 - 20 of 44 results

  1. CRISPR References and Information

    Type
    Collection
    ...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for...tool helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs, as well ...for CRISPR/Cas design (Link opens in a new window) Program for designing optimal gRNAs. Provides feedback...optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and ssODNs design and cloning...window) CRISPR/Cas9 reagent generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer ...

  3. CRISPR Plasmids - Yeast

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use ...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus...

  4. Plan Your Experiment

    Type
    Collection
    ...about how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you have...planning to modify prior to designing your gRNA, as sequence variation between your gRNA targeting sequence and...associated gRNAs based on their predicted on-target and off-target activity ( see gRNA design software ...Genetic Manipulation Select Expression System Design gRNA Publications Get Started CRISPR is a powerful...enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence ...delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then... Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are combined...

  5. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to target ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...pyogenes , S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids... questions to consider when choosing a gRNA: Does a given gRNA sequence exactly match your genomic target...Variation between a given gRNA sequence and your genomic target may reduce the gRNA activity. The variety ...published and unpublished plasmids containing gRNA sequences designed to be used with any CRISPR nuclease and...

  6. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... in Xenopus. Cut Fully functional Cas9 enzymes designed to introduce a double-strand break (DSBs) at a... a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially repaired...

  7. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus...

  8. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use ...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR plasmids that have been designed for use in C. elegans. CRISPR...

  9. CRISPR Plasmids and Resources

    Type
    Collection
    ...Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links ...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment...experiment based on a variety of factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids...Bacteria CRISPR plasmids designed for use in bacteria. C. elegans CRISPR plasmids designed for use in C. elegans... CRISPR plasmids designed for use in Drosophila . Mammalian CRISPR plasmids designed for use in mammalian...Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids designed for use in plants. Yeast...Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed for use in Xenopus . ...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use ...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...

  11. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use ...Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...

  12. CRISPR Plasmids - Plants

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use ...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...

  13. CRISPR Plasmids - Parasites

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic parasites ...Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that binds...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...post . Need help designing a pegRNA? Try the DeepPrime (Link opens in a new window) design tool from Hyongbum...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is a “search...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...PEmax to enhance pegRNA stability epegRNA - additional protection added to 3’ tail of pegRNA to prevent RNA...

  15. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive...is easy to implement due to the simplicity of gRNA design, programmable for different genomic loci, capable...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers...efficiently visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity...

  16. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...

  17. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...

  18. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying molecules...genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification...interest in vivo. For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus...of any genomic sequence specified by a particular gRNA. In the enChIP (engineered DNA-binding molecule-...

  19. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted epigenetic...catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer ...

  20. CRISPR Plasmids - Tagging

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system has...containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The...currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your ...Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor CEBPB Human FLAG... for more information about HDR/repair template design. How to use CRISPR to tag your gene of interest...
Showing: 1 - 20 of 44 results