Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 34 results for: yeast expression

Showing: 1 - 20 of 34 results

  1. Microbiology Resources

    Type
    Collection
    ...Vectors - Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - ...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers,...and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for...Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources : Find...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab Bacterial Fluorescence Labeling :...- Geertsma and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system -...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... - Mammalian Expression pKT0090 - Yeast Expression mVenus-pBAD - Bacterial Expression SYFP2 515 527 69... Bacterial Expression pJL-mGold2t - Yeast Expression pCaggs-mGold2t - Mammalian Expression mCitrine 516...pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1 - Mammalian Expression tdTomato-C1 - Mammalian Expression tdTomato-pBAD...Mammalian Expression pFA6a-link-yomRuby2-SpHis5 - Yeast Expression mRuby2-N1 - Mammalian Expression mRuby2...Mammalian Expression pTagRFP657-C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression vector URA3 Yeast pAG306GAL-ccdB... to express a gene; however, we have included a function section for if you want to stably express an ...genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion Protein...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ... Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with...

  4. CRISPR Plasmids - Yeast

    Type
    Collection
    ... CRISPR Yeast CRISPR Plasmids: Yeast Browse CRISPR Plasmids By Function...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...have been designed for use in yeast. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes will introduce...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus...by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI...Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4 Zaratiegui pJZC588 62315 Yeast none S. pyogenes URA3...gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI yes, cut S. ...Wyrick pML107 67639 Yeast BclI-SwaI yes, cut S. pyogenes LEU2 Wyrick pT040 67640 Yeast BclI-SwaI none S....Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. ...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted...

  6. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Addgene Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast Localization and Overexpression... Depositor Collections Botman-Teusink Yeast FP Collection Botman-Teusink Yeast FP Collection... . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized...Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs in yeast. Vectors...Collection FP Tagging FP Overexpression Resources This comprehensive collection of yeast codon-optimized fluorescent...for constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization...proteins in yeast. Depositor...

  7. CRISPR Plasmids and Resources

    Type
    Collection
    ...designed for use in plants. Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed...organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans, parasites, and... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...

  8. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the... plasmids for activating or upregulating gene expression in various model organisms. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional...Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  9. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR Plasmids: Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. This interference... Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  10. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian... ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do...

  11. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... CRISPR: Purify Genomic Loci Mammalian Bacteria Yeast Resources Identifying molecules associated with ...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system... Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  12. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems.... enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene partnered...nsp3, OC43, NL63, HKU1, and 229E) Expression type (e.g. bacterial, yeast, or mammalian) Tags (e.g. 6xHis...to Industry page. These plasmids have not been expressed or functionally validated by Ginkgo or Addgene... V5) Mutations Wild type (wt) Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc...Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems Collection: COVID-19 and Coronavirus ...

  13. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors ...Further advancements have been made by optimizing expression of the fusions, modifying the linker region between... Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  14. Depositor Collections

    Type
    Collection
    ...Plasmid Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate...to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant phosphoproteins...CRISPR, disease research, visualization, protein expression, synthetic biology, and viral vector plasmids...Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic...

  15. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  16. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  17. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  18. Institute for Protein Innovation

    Type
    Collection
    ...tags, as well as antibody plasmids to create or express plasmid-based antibodies. The majority of antibodies... platform (Link opens in a new window) based on yeast display technology (Link opens in a new window) ...The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid Description...

  19. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  20. Adenovirus Plasmids

    Type
    Collection
    ...kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target...Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune response induced...cells or microorganisms, including bacteria and yeast. Two plasmids, termed a shuttle plasmid and an adenoviral...Block 6 with deletion in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral...Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production...For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without...
Showing: 1 - 20 of 34 results