Viral Vector Packaging Service: Molecular Tools
- Tetracycline Transactivators
- Affinity Purification
- Neurophysiology
- Cell Ablation
- Genome Engineering
- Labeling
- Overexpression
Tetracycline Transactivators and Inducible Tools
These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression systems.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 99118 | pAAV-CAG-tTA | CAG-driven, constitutive | Expression of the tet-off transactivator (tTA) | 2 | Viviana Gradinaru |
| 99120 | pAAV-ihSyn1-tTA | Inducible Synapsin promoter (ihSyn) | Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. | 1 | Viviana Gradinaru |
| 99121 | pAAV-ihSyn1-DIO-tTA | Cre-dependent and inducible Synapsin promoter (ihSyn) | Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. | 1 | Viviana Gradinaru |
| 117383 | TRE-DIO-eYFP | Cre-dependent and Tetracycline-inducible | Cre-dependent, Tet-inducible expression of EYFP | 1 | Viviana Gradinaru |
Tools for Affinity Purification
These AAV encode tools for affinity purification (which can be used for viral TRAP).
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 98747 | pAAV-FLEX-EGFPL10a | EF1a-driven and Cre-dependent | EGFP-tagged ribosomal L10a | 5, rg* | Nathaniel Heintz, Alexander Nectow, Eric Schmidt |
Neurophysiology Tools
These AAV encode tools that can be used for neurophysiology experiments, including neuronal connectivity labeling.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 51509 | AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE | Syn-driven, Cre-dependent | Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP for labeling of axon terminals. | 1 | Hongkui Zeng |
| 71760 | pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby | Syn-driven, Cre-dependent | Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals. | 1 | Liqun Luo |
| 60658 | pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato | EF1a-driven, Flp-dependent | Flp-dependent expression of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato | 8 | Massimo Scanziani |
| 34910 | paavCAG-pre-mGRASP-mCerulean | CAG-driven, constitutive | Expression of pre-synaptic mGRASP-mCerulean for synaptic connectivity mapping | 5 | Jinhyun Kim |
| 34912 | paavCAG-post-mGRASP-2A-dTomato | CAG-driven, constitutive | Expression of post-synaptic mGRASP-dTomato for synaptic connectivity mapping | 5 | Jinhyun Kim |
Tools for Cell Ablation
These AAV encode tools that can be used for cell ablation.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 124364 | pAAV-FLEX-DTR-GFP | CBA-driven, Cre-dependent | Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation. | 2 | Thomas Jessell, Eiman Azim |
| 45580 | pAAV-flex-taCasp3-TEVp | EF1a-driven, Cre-dependent | Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. | 1, 5 | Nirao Shah, Jim Wells |
Genome Engineering Tools
These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 61592 | pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA | CMV-driven, constitutive | Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). | 6 | Feng Zhang |
Labeling Tools
These AAV encode tools that can be used for cell or protein labeling.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 149415 | pAAV-TBG-ER-TurboID | TBG-driven, constitutive | Expression of ER-localized TurboID (with HA and V5 tag). | 8 | Jonathan Long |
| 160857 | pAAV-FLEx-ER-TurboID | CMV-driven, Cre-dependent | Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). | 8 | Jonathan Long |
Overexpression Tools
These AAV encode various genes for overexpression.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 58376 | pAAV/D377Y-mPCSK9 | hAAT-driven, constitutive | Expression of mutant (D377Y) murine PCSK9 for studying hypercholesterolemia and atherosclerosis. | 8 | Jacob Bentzon |
| 169128 | pAAV-DIO-EF1α-mKate2-PDE4D3-Cat | EF1a-driven, Cre-dependent | Cre-dependent expression of mKate2 and a truncated human PDE4D3 | 1 | Georg Nagel |
| 176267 | pAAV-FLEX-P301L Tau | EF1a-driven, Cre-dependent | Cre-dependent expression of human 2N4R microtubule-associated protein tau with P301L mutation | 1 | Tsuneya Ikezu |
Don’t See What You’re Looking For?
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