Viral Vector Packaging Service: Molecular Tools
- Tetracycline Transactivators
- Affinity Purification
- Neurophysiology
- Cell Ablation
- Genome Engineering
- Labeling
- Overexpression
Tetracycline Transactivators and Inducible Tools
These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression systems.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 99118 | pAAV-CAG-tTA | CAG-driven, constitutive | Expression of the tet-off transactivator (tTA) | 2 | Gradinaru |
| 99120 | pAAV-ihSyn1-tTA | Inducible Synapsin promoter (ihSyn) | Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. | 1 | Gradinaru |
| 99121 | pAAV-ihSyn1-DIO-tTA | Cre-dependent and inducible Synapsin promoter (ihSyn) | Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. | 1 | Gradinaru |
| 117383 | TRE-DIO-eYFP | Cre-dependent and Tetracycline-inducible | Cre-dependent, Tet-inducible expression of EYFP | 1 | Gradinaru |
Tools for Affinity Purification
These AAV encode tools for affinity purification (which can be used for viral TRAP).
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 98747 | pAAV-FLEX-EGFPL10a | EF1a-driven and Cre-dependent | EGFP-tagged ribosomal L10a | 5, rg* | Heintz, Nectow, Schmidt |
Neurophysiology Tools
These AAV encode tools that can be used for neurophysiology experiments, including neuronal connectivity labeling.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 51509 | AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE | Syn-driven, Cre-dependent | Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP for labeling of axon terminals. | 1 | Zeng |
| 71760 | pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby | Syn-driven, Cre-dependent | Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals. | 1 | Luo |
| 60658 | pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato | EF1a-driven, Flp-dependent | Flp-dependent expression of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato | 8 | Scanziani |
| 34910 | paavCAG-pre-mGRASP-mCerulean | CAG-driven, constitutive | Expression of pre-synaptic mGRASP-mCerulean for synaptic connectivity mapping | 5 | Kim |
| 34912 | paavCAG-post-mGRASP-2A-dTomato | CAG-driven, constitutive | Expression of post-synaptic mGRASP-dTomato for synaptic connectivity mapping | 5 | Kim |
Tools for Cell Ablation
These AAV encode tools that can be used for cell ablation.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 124364 | pAAV-FLEX-DTR-GFP | CBA-driven, Cre-dependent | Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation. | 2 | Jessell, Azim |
| 45580 | pAAV-flex-taCasp3-TEVp | EF1a-driven, Cre-dependent | Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. | 1, 5 | Shah, Wells |
Genome Engineering Tools
These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 61592 | pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA | CMV-driven, constitutive | Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). | 6 | Zhang |
Labeling Tools
These AAV encode tools that can be used for cell or protein labeling.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 149451 | pAAV-TBG-ER-TurboID | TBG-driven, constitutive | Expression of ER-localized TurboID (with HA and V5 tag). | 8 | Long |
| 160857 | pAAV-FLEx-ER-TurboID | CMV-driven, Cre-dependent | Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). | 8 | Long |
Overexpression Tools
These AAV encode various genes for overexpression.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 58376 | pAAV/D377Y-mPCSK9 | hAAT-driven, constitutive | Expression of mutant (D377Y) murine PCSK9 for studying hypercholesterolemia and atherosclerosis. | 8 | Bentzon |
| 176267 | pAAV-FLEX-P301L Tau | EF1a-driven, Cre-dependent | Cre-dependent expression of human 2N4R microtubule-associated protein tau with P301L mutation | 1 | Ikezu |
Don’t See What You’re Looking For?
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Or fill out our form to let us know what you would like to see available in viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be helpful to the scientific community.