Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use the CRISPR/Cas9 technology in your own lab.
|Cut||Wild type Cas9 efficiently generates double strand breaks (DSBs) at sequences homologous to co-expressed gRNA.|
|Nick||A mutated "nickase" version of the Cas9 enzyme generates a single-strand DNA break (Nick), instead of a double-strand DNA break (Cut).|
|Interfere||A catalytically inactive Cas9 (dCas9) can knockdown gene expression by interfering with transcription. The dCas9 can sometimes be fused to an additional repressor peptide.|
|Activate||A catalytically inactive Cas9 (dCas9) fused to an activator peptide can activate or increase gene expression.|
|dCas9-FokI||A catalytically inactive Cas9 (dCas9) fused to FokI nuclease to generate double strand breaks (DSBs, Cut) at sequences homologous to two co-expressed gRNA.|
|Purify||Isolate specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused with an epitope tag(s).|
|Visualize||Visualize specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused to a fluorescent protein.|
|Screen||Use pooled CRISPR libraries to screen for genes involved in specific biological processes.|
|Validated gRNAs||Experimentally validated, pre-made gRNA plasmids to specific genes.|
|Empty gRNA Vectors||Select a gRNA plasmid based on a variety of factors, such as selectable marker or cloning method.|
CRISPRs for use in Mammalian systems are listed under the Browse by Function links above.
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12. (Article). SEE PLASMIDS
Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. (Article). SEE PLASMIDS
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS. Cell. 2013 Jul 9. doi: 10.1016/j.cell.2013.06.044. (Article). SEE PLASMIDS