1

CRISPR Cas Plasmids and Protocols

Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use the CRISPR/Cas9 technology in your own lab.

Browse CRISPR Plasmids by Function

Cut Wild type Cas9 efficiently generates double strand breaks (DSBs) at sequences homologous to co-expressed gRNA.
Nick A mutated "nickase" version of the Cas9 enzyme generates a single-strand DNA break (Nick), instead of a double-strand DNA break (Cut).
Interfere A catalytically inactive Cas9 (dCas9) can knockdown gene expression by interfering with transcription. The dCas9 can sometimes be fused to an additional repressor peptide.
Activate A catalytically inactive Cas9 (dCas9) fused to an activator peptide can activate or increase gene expression.
dCas9-FokI A catalytically inactive Cas9 (dCas9) fused to FokI nuclease to generate double strand breaks (DSBs, Cut) at sequences homologous to two co-expressed gRNA.
Purify Isolate specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused with an epitope tag(s).
Visualize Visualize specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused to a fluorescent protein.
Screen Use pooled CRISPR libraries to screen for genes involved in specific biological processes.
Empty gRNA Vectors Select a gRNA plasmid based on a variety of factors, such as selectable marker or cloning method.

Browse Plasmids by Model Organism

CRISPRs for use in Mammalian systems are listed under the Browse by Function links above.

Mammalian Bacteria Drosophila Plant
C. elegans Yeast Zebrafish Xenopus

Top CRISPR Articles based on Frequency of Requests

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12. (Article). SEE PLASMIDS

Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3 (Article). SEE PLASMIDS

Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. (Article). SEE PLASMIDS

Genetic Screens in Human Cells Using the CRISPR/Cas9 System. Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2013 Dec 12. (Article). SEE PLASMIDS

RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. (Article). SEE PLASMIDS

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS. Cell. 2013 Jul 9. doi: 10.1016/j.cell.2013.06.044. (Article). SEE PLASMIDS