CRISPR main page header CRISPR/Cas Plasmids and Protocols


Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use the CRISPR/Cas9 technology in your own lab.

Browse CRISPR Plasmids by Function

CRISPR cut cartoon button Cut Wild type Cas9 efficiently generates double strand breaks (DSBs) at sequences homologous to co-expressed gRNA.
CRISPR nick cartoon button Nick A mutated "nickase" version of the Cas9 enzyme generates a single-strand DNA break (Nick), instead of a double-strand DNA break (Cut).
CRISPR interfere cartoon button Interfere A catalytically inactive Cas9 (dCas9) can knockdown gene expression by interfering with transcription. The dCas9 can sometimes be fused to an additional repressor peptide.
CRISPR activate cartoon button Activate A catalytically inactive Cas9 (dCas9) fused to an activator peptide can activate or increase gene expression.
CRISPR cut cartoon button dCas9-FokI A catalytically inactive Cas9 (dCas9) fused to FokI nuclease to generate double strand breaks (DSBs, Cut) at sequences homologous to two co-expressed gRNA.
CRISPR purify cartoon button Purify Isolate specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused with an epitope tag(s).
CRISPR visualize cartoon button Visualize Visualize specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused to a fluorescent protein.
CRISPR libraries cartoon button Screen Use pooled CRISPR libraries to screen for genes involved in specific biological processes.
empty gRNA cartoon button Validated gRNAs Experimentally validated, pre-made gRNA plasmids to specific genes.
Round_Empty_gRNA_Expression_Icon.png Empty gRNA Vectors Select a gRNA plasmid based on a variety of factors, such as selectable marker or cloning method.

Browse Plasmids by Model Organism

CRISPRs for use in Mammalian systems are listed under the Browse by Function links above.

Mammalian Bacteria Drosophila Plant
C. elegans Yeast Zebrafish Xenopus

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