Skip to main content
Addgene
Showing: 1 - 40 of 547 results

  7. pMCh-623

    Plasmid
    #92266
    Purpose
    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells
    Depositor
    Witold Filipowicz, Marina Chekulaeva
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    dGW182 6W6A (gw Fly)
    Use
    Tags
    NHA
    Expression
    Insect
    Mutation
    The following point mutations were introduced W23…
    Promoter
    Ac5
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92266-plasmid-map-sequence-id-194528

    Enlarge

  11. pMCh-660

    Plasmid
    #92259
    Purpose
    Plasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells
    Depositor
    Witold Filipowicz, Marina Chekulaeva
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    TNRC6C CED (TNRC6C Human)
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    C-terminal effector domain (CED): aa 1369-1690 wi…
    Promoter
    CMV
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    92259-plasmid-map-sequence-id-194509

    Enlarge

  14. pMCh-665

    Plasmid
    #92254
    Purpose
    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells
    Depositor
    Witold Filipowicz, Marina Chekulaeva
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    TNRC6C CED (TNRC6C Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    C-terminal effector domain (CED): aa 1369-1690 wi…
    Promoter
    EF1A
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92254-plasmid-map-sequence-id-194411

    Enlarge

  16. pMCh-646

    Plasmid
    #92289
    Purpose
    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells
    Depositor
    Witold Filipowicz, Marina Chekulaeva
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    dGW182 CED 7W7A (gw Fly)
    Use
    Tags
    NHA
    Expression
    Insect
    Mutation
    dGW182 CED: aa 940-1215 with the following point …
    Promoter
    Ac5
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92289-plasmid-map-sequence-id-194379

    Enlarge

  20. pMCh-1474

    Plasmid
    #82420
    Purpose
    Plasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 NED
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1159-1698 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82420-plasmid-map-sequence-id-156264

    Enlarge

  21. pMCh-1475

    Plasmid
    #82421
    Purpose
    Plasmid for expression of HA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-1158 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82421-plasmid-map-sequence-id-156262

    Enlarge

  22. pMCh-1476

    Plasmid
    #82422
    Purpose
    Plasmid for expression of NHA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 NED
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1159-1698 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82422-plasmid-map-sequence-id-156258

    Enlarge

  23. pMCh-1477

    Plasmid
    #82423
    Purpose
    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-1158 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82423-plasmid-map-sequence-id-156255

    Enlarge

  25. pMCh-1479

    Plasmid
    #82425
    Purpose
    Plasmid for expression of GST-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 NED
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    deleted amino acids 1159-1698 from nvGW182
    Promoter
    EF1A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82425-plasmid-map-sequence-id-156246

    Enlarge

  26. pMCh-1480

    Plasmid
    #82426
    Purpose
    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted
    Promoter
    EF1A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82426-plasmid-map-sequence-id-156240

    Enlarge

  27. pMCh-1498

    Plasmid
    #82427
    Purpose
    Plasmid for expression of NHA-tagged Nematostella vectensis GW182 11W11A in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 11W11A
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    Trp 1167, 1183, 1227, 1309, 1391, 1453, 1574, 158…
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82427-plasmid-map-sequence-id-156194

    Enlarge

  28. pMCh-1522

    Plasmid
    #82428
    Purpose
    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED 11W11A
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted and Tr…
    Promoter
    EF1A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82428-plasmid-map-sequence-id-156193

    Enlarge

  29. pMCh-1526

    Plasmid
    #82429
    Purpose
    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED 11W11A
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted and Tr…
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82429-plasmid-map-sequence-id-156191

    Enlarge

  32. pMCh-1540

    Plasmid
    #82433
    Purpose
    Plasmid for expression of GST-tagged human-Nematostella GW182 11W11A chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 11W11A chimera
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    EF1A
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82433-plasmid-map-sequence-id-156169

    Enlarge

  33. pMCh-1539

    Plasmid
    #82432
    Purpose
    Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 chimera
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    EF1A
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82432-plasmid-map-sequence-id-156170

    Enlarge

  34. pMCh-1538

    Plasmid
    #82431
    Purpose
    Plasmid for expression of NHA-tagged human-Nematostella GW182 11W11A chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 11W11A chimera
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    CMV
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82431-plasmid-map-sequence-id-156186

    Enlarge

  35. pMCh-1537

    Plasmid
    #82430
    Purpose
    Plasmid for expression of NHA-tagged human-Nematostella GW182 chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 chimera
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    CMV
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82430-plasmid-map-sequence-id-156190

    Enlarge

Showing: 1 - 40 of 547 results