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ssFv_lenti_wtATMSPARK2
Plasmid#195249Purposeexpress wtATMSPARK2 in a lenti vectorDepositorInsertATMSPARK2 sensor
UseLentiviralTagsExpressionMammalianMutationPromoterssfvAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
EE-LRRK2
Plasmid#193583PurposeExpresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminusDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsFYVE-CC2 and 3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRK248
Plasmid#173755Purposeco-injection plasmidDepositorInsertPrps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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ARK2aa
Plasmid#81013PurposeE.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-aphA
and PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSRK2721
Plasmid#78572PurposeGateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusionsDepositorInsertGateway cloning cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
ssFv_lenti_mutATMSPARK2
Plasmid#195250Purposeexpress Thr to Ala mutant ATMSPARK2 in a lenti vectorDepositorInsertThr to Ala mutant ATMSPARK2
UseLentiviralTagsExpressionMammalianMutationPromoterssfvAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRK261
Plasmid#173754PurposeCeLINC plasmidDepositorInsert(empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJRK249
Plasmid#173747PurposeCeLINC plasmidDepositorInsertPrps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPrps-0Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_LRRK2-3
Plasmid#199272PurposepegRNA used to induce LRRK2 (c. 6055 G > A) mutationDepositorInsertLRRK2 6055GtoA pegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRK260
Plasmid#173753PurposeCeLINC plasmidDepositorInsertPrps-0::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPrps-0Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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LRRK2_Halo_N_allele
Plasmid#178142PurposeDonor vector for endogenous tagging of human LRRK2 at the N-terminus with halotagInsertHalotag (LRRK2 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
DYRK2_HUMAN_D0
Plasmid#79729PurposeThis plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK2 (DYRK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
VRK2_HUMAN_D0
Plasmid#79737PurposeThis plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK2 (VRK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJRK246
Plasmid#173741Purposedonor plasmid for SapTrap assemblyDepositorInsertwrt-2 promoter
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK253
Plasmid#173750PurposeCeLINC plasmidDepositorInsertPhsp-16.48::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPhsp-16.48Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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LRRK2_1867_2136
Plasmid#40408DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867_2136PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
LRRK2_1867_2161_F1908A_L1936R_I2131Q
Plasmid#40421DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; F1908A, L1936R, I2131QPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJRK254
Plasmid#173742Purposedonor plasmid for SapTrap assemblyDepositorInsertVHH(mCherry)
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LRRK2_1867_2143
Plasmid#40407DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867_2143PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
LRRK2_1867_2161_L1914A
Plasmid#40436DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; L1914APromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
sgMARK2-3-3
Plasmid#229442Purposeknockout of MARK2 and MARK3DepositorInsertsgMARK2-3-3 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-3-2
Plasmid#229441Purposeknockout of MARK2 and MARK3DepositorInsertsgMARK2-3-2 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-3-1
Plasmid#229440Purposeknockout of MARK2 and MARK3DepositorInsertsgMARK2-3-1 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-3
Plasmid#229436Purposeknockout of MARK2DepositorInsertsgMARK2-R-3 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-2
Plasmid#229435Purposeknockout of MARK2DepositorInsertsgMARK2-R-2 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-1
Plasmid#229434Purposeknockout of MARK2DepositorInsertsgMARK2-R-1 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRK247
Plasmid#173734Purposedonor plasmid for SapTrap assemblyDepositorInsertCRY2(WT)
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK258
Plasmid#173751Purposefluorescent par-6(WT) expressionDepositorInsertPrps-0::mKate2::par-6::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPrps-0Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK252
Plasmid#173749PurposeCeLINC plasmidDepositorInsertPelt-2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPelt-2Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK259
Plasmid#173752Purposefluorescent par-6(delPB1) expressionDepositorInsertPrps-0::mKate2:: par-6(_PB1)::unc-54 3' UTR
UseTagsExpressionWormMutationdelPB1PromoterPrps-0Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK251
Plasmid#173748PurposeCeLINC plasmidDepositorInsertPwrt-2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPwrt-2Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARK2a
Plasmid#81017PurposeE.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco
PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
LRRK2_1867_2134
Plasmid#40409DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867_2134PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
LRRK2_1867_2170
Plasmid#40410DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867_2170PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits
