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Plasmid#111085PurposepNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectorsDepositorInsertempty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
UseDeleted foamy virusTagsExpressionMutationat nt 2334 A changed to T, at nt 2495 A changed t…PromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR4_miniFXN_8
Plasmid#234361PurposeMini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.DepositorInsertFXN (FXN Human)
UseCloningTagsFlag TagExpressionMutationThere is a partial deletion in the 5'UTR of …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR4_miniFXN_7
Plasmid#234360PurposeMini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.DepositorInsertFXN (FXN Human)
UseCloningTagsFlag TagExpressionMutationThere is a partial deletion in the 5'UTR of …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Vibrio_vulnificus
Plasmid#224405PurposeFor expression of the type I CBASS system encoded by Vibrio vulnificusDepositorInsertEndonuclease_Phospholipase_VvCdnG_AGS-C
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CTDSP2_NIF
Plasmid#109831PurposeProtein expression and purification of CTDSP2_NIFDepositorInsertCTDSP2_NIF (CTDSP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
nifF in pET21b
Plasmid#124520PurposeAzotobacter vinelandii flavodoxin II of NitrogenaseDepositorInsertFlavodoxin II (nifF Azotobacter vinelandii)
UseTagsNoneExpressionBacterialMutationPromoterT7 / lacAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCiPS-NIFV
Plasmid#110807PurposeNon-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cellsDepositorInsertPol
UseTagsExpressionMammalianMutation2 mutations in the DDE catalyic core motif of the…PromoterCMVAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-SNIFP-N1
Plasmid#197189PurposeExpresses the fusion protein of CytERM-SNIFP in mammalian cellsDepositorInsertCytERM-SNIFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX_Snifit
Plasmid#119043PurposePlasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)DepositorInsertMTX_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-cyto
Plasmid#113917PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-mito
Plasmid#113919PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-mito
Plasmid#113916PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-nucl
Plasmid#113918PurposePlasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-cyto
Plasmid#113914PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-nucl
Plasmid#113915PurposePlasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SNIFP
Plasmid#197210PurposeExpresses the protein of SNIFP in mammalian cellsDepositorInsertSNIFP
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u36_Snifit
Plasmid#119045PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)DepositorInsertMTX-u36_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation36TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u51_Snifit
Plasmid#119046PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)DepositorInsertMTX-u51_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation51TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u23_Snifit
Plasmid#119044PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)DepositorInsertMTX-u23_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation23TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NAD-Snifit
Plasmid#113658PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentrationDepositorInsertNAD-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NADP-Snifit
Plasmid#113913PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+DepositorInsertNADP-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CoA-Snifit(G41)
Plasmid#197945PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CoA-Snifit(V97T)
Plasmid#197947PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-V97T, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_CoA-Snifit(G41)
Plasmid#197939PurposeExpression of CoA-Snifit in bacterial cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionBacterialMutationPanK=PanK-D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_CoA-Snifit(G41S)
Plasmid#197940PurposeExpression of CoA-Snifit in bacterial cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionBacterialMutationPanK=PanK-G41S, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_CoA-Snifit(V97T)
Plasmid#197941PurposeExpression of CoA-Snifit in bacterial cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionBacterialMutationPanK=PanK-V97T, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CoA-Snifit(V97T)
Plasmid#197944PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-V97T, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CoA-Snifit(G41)
Plasmid#197942PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SNIFP-P2A-GFP
Plasmid#197157PurposeExpresses the protein of SNIFP-P2A-GFP in mammalian cellsDepositorInsertSNIFP-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_COX8-CoA-Snifit(G41S)
Plasmid#197946PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-G41S, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)
Plasmid#197943PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-G41S, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human ubiquitination-related proteins CRISPR KO library
Pooled Library#174592PurposeCRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shNFS1_1
Plasmid#102963PurposeSuppression of NFS1DepositorInsertshNFS1_1 (NFS1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shNFS1_2
Plasmid#102964PurposeSuppression of NFS1DepositorInsertshNFS1_2 (NFS1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKC530 pBABE EGFP-ZNF335
Plasmid#192698PurposeRetroviral transgene expression of human ZNF335DepositorInsertZNF335 (ZNF335 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shNFS1_4
Plasmid#102966PurposeSuppression of NFS1DepositorInsertshNFS1_4 (NFS1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shNFS1_3
Plasmid#102965PurposeSuppression of NFS1DepositorInsertshNFS1_3 (NFS1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro NFS1
Plasmid#102977PurposeExpression of NFS1DepositorInsertNFS1 (NFS1 Human)
UseRetroviralTagsExpressionMammalianMutationSilent mutations to eliminate shNFS1_1 binding si…PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLENTICRISPR sgNFS1
Plasmid#102979PurposeCutting NFS1 genomic locusDepositorInsertsgNFS1 (NFS1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro NFS1 CD
Plasmid#102978PurposeExpression of NFS1 C381A mutantDepositorInsertNFS1 C381A (NFS1 Human)
UseRetroviralTagsExpressionMammalianMutationC381A catalytic site mutant, Silent mutations to …PromoterAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only