We narrowed to 218 results for: pcmv gfp plasmid

1. pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1)

   Plasmid

   #233273

   Purpose

   To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1

   Depositor

   Glenn Schafe, Jonathan Ploski

   Insert

   GFP and shRNA targeting Rat Nurr1 (Nr4a2 Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #5)

   Plasmid

   #233274

   Purpose

   To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1

   Depositor

   Glenn Schafe, Jonathan Ploski

   Insert

   GFP and shRNA targeting Rat Nurr1 (Nr4a2 Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PNCA-GFP

   Plasmid

   #67789

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

   Insert

   MMLV-LTR-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

4. PNCA-GFP-dYY1

   Plasmid

   #67791

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

   Insert

   MMLV-LTR-GFP
   

   Use

   Tags

   Expression

   Mutation

   Deletion of LTR U3 YY1 binding domain

   Promoter

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PEA1-GFP

   Plasmid

   #171993

   Purpose

   Delivers all prime editing nickase components in a single, GFP selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEA1-Nuclease-GFP

   Plasmid

   #171994

   Purpose

   Delivers all prime editing nuclease components in a single, GFP selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1)

   Plasmid

   #233270

   Purpose

   To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

   Depositor

   Glenn Schafe, Jonathan Ploski

   Article

   Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

   Insert

   GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #5)

   Plasmid

   #233271

   Purpose

   To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

   Depositor

   Glenn Schafe, Jonathan Ploski

   Article

   Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

   Insert

   GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMV+ plasmid

    Plasmid

    #169740

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Equalizer-L plasmid

    Plasmid

    #169732

    Purpose

    Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Equalizer-H plasmid

    Plasmid

    #169367

    Purpose

    Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Equalizer-M plasmid

    Plasmid

    #169731

    Purpose

    Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p3991

    Plasmid

    #198149

    Purpose

    Mammalian expression of LAMP1

    Depositor

    Michael Hensel

    Insert

    PCMV::LAMP1::HaloTag::GFP (LAMP1 Human)
    

    Use

    Tags

    HaloTag-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV+ plasmid with onboard mCherry cassette

    Plasmid

    #169741

    Purpose

    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV+ episome

    Plasmid

    #169742

    Purpose

    CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Bxb1-surrogate-reporter

    Plasmid

    #65624

    Purpose

    Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attP
    

    Use

    Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Equalizer-L episome

    Plasmid

    #169738

    Purpose

    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. miniCGBE1 (pBM1219)

    Plasmid

    #140253

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge