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Showing: 361 - 400 of 447 results

  1. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  5. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-260007

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  6. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199221-plasmid-map-sequence-id-396541

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  7. pGFP_1

    Plasmid
    #238192
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238192-plasmid-map-sequence-id-472672

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  8. pSNAP_24

    Plasmid
    #238199
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAPtag-FTH1(24-mer), under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag-FTH1(24mer) and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238199-plasmid-map-sequence-id-472676

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  9. pSNAP_6

    Plasmid
    #238198
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAPtag-6mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag-6mer and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238198-plasmid-map-sequence-id-472674

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  10. pSNAP_1

    Plasmid
    #238196
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAP-tag, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238196-plasmid-map-sequence-id-472671

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  11. pGFP_24

    Plasmid
    #238195
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP-FTH1(24-mer), under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-FTH1(24mer) and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238195-plasmid-map-sequence-id-472670

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  12. pSNAP_4

    Plasmid
    #238197
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAPtag-4mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag-4mer and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238197-plasmid-map-sequence-id-472669

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  13. pGFP_4

    Plasmid
    #238193
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP-4mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-4mer and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238193-plasmid-map-sequence-id-472663

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  14. pCMV_GFP_24

    Plasmid
    #238190
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP and FTH1 (24-mer), under a CMV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-FTH1 and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238190-plasmid-map-sequence-id-472656

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  15. pGFP_6

    Plasmid
    #238194
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP-6mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-6mer and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238194-plasmid-map-sequence-id-472655

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  16. pCMV_SNAP_24

    Plasmid
    #238191
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAP-tag and FTH1 (24-mer), under a CMV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAP-FTH1 and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238191-plasmid-map-sequence-id-472654

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Showing: 361 - 400 of 447 results